Restriction map for
pFN21AE1187
(Linear) (Six-base) MAPSORT of: KIEE1187.seq Check: 5906 from: 1 to: 2295
KIEE1187.orf
With 182 enzymes: SgfI *
July 3, 2012 16:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 550 2295
Size: 550 1745
AatII G_ACGT'C
Cuts at: 0 1397 2295
Size: 1397 898
AclI AA'CG_TT
Cuts at: 0 505 2295
Size: 505 1790
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 946 964 1079 1148 1150 1455 2295
Size: 575 371 18 115 69 2 305 840
Fragments arranged by size:
840 575 371 305 115 69 18 2
AflIII A'CryG_T
Cuts at: 0 1184 1690 1859 2295
Size: 1184 506 169 436
Fragments arranged by size:
1184 506 436 169
AleI CACnn'nnGTG
Cuts at: 0 1437 1439 1864 2168 2295
Size: 1437 2 425 304 127
Fragments arranged by size:
1437 425 304 127 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 542 628 737 2295
Size: 542 86 109 1558
Fragments arranged by size:
1558 542 109 86
AlwNI CAG_nnn'CTG
Cuts at: 0 42 234 361 1387 2295
Size: 42 192 127 1026 908
Fragments arranged by size:
1026 908 192 127 42
ApoI r'AATT_y
Cuts at: 0 165 1095 1577 1914 1927 2295
Size: 165 930 482 337 13 368
Fragments arranged by size:
930 482 368 337 165 13
AvaI C'yCGr_G
Cuts at: 0 805 1822 2295
Size: 805 1017 473
Fragments arranged by size:
1017 805 473
BamHI G'GATC_C
Cuts at: 0 2016 2295
Size: 2016 279
BanII G_rGCy'C
Cuts at: 0 1777 1796 1840 2295
Size: 1777 19 44 455
Fragments arranged by size:
1777 455 44 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 1136 1154 1509 2269 2295
Size: 1136 18 355 760 26
Fragments arranged by size:
1136 760 355 26 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1561 1595 2295
Size: 1561 34 700
Fragments arranged by size:
1561 700 34
BfrBI ATG'CAT
Cuts at: 0 791 2295
Size: 791 1504
BglI GCCn_nnn'nGGC
Cuts at: 0 1943 2295
Size: 1943 352
BglII A'GATC_T
Cuts at: 0 261 1361 1697 2295
Size: 261 1100 336 598
Fragments arranged by size:
1100 598 336 261
BlpI GC'TnA_GC
Cuts at: 0 1795 2295
Size: 1795 500
BmgBI CAC'GTC
Cuts at: 0 1862 2295
Size: 1862 433
BmrI ACTGGGnnnn_n'
Cuts at: 0 686 1682 1900 2295
Size: 686 996 218 395
Fragments arranged by size:
996 686 395 218
BmtI G_CTAG'C
Cuts at: 0 1046 2257 2295
Size: 1046 1211 38
Fragments arranged by size:
1211 1046 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 817 1807 2217 2295
Size: 19 798 990 410 78
Fragments arranged by size:
990 798 410 78 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 1250 1804 2295
Size: 1250 554 491
Fragments arranged by size:
1250 554 491
BsaHI Gr'CG_yC
Cuts at: 0 872 1394 2295
Size: 872 522 901
Fragments arranged by size:
901 872 522
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1669 1699 1834 1864 2295
Size: 1669 30 135 30 431
Fragments arranged by size:
1669 431 135 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1804 2295
Size: 1804 491
BseYI C'CCAG_C
Cuts at: 0 833 2091 2295
Size: 833 1258 204
Fragments arranged by size:
1258 833 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 2295
Size: 588 1707
BsiHKAI G_wGCw'C
Cuts at: 0 812 1490 1733 1796 1840 2295
Size: 812 678 243 63 44 455
Fragments arranged by size:
812 678 455 243 63 44
BsmI GAATG_Cn'
Cuts at: 0 133 793 1332 2295
Size: 133 660 539 963
Fragments arranged by size:
963 660 539 133
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1108 2295
Size: 1108 1187
Bsp1286I G_dGCh'C
Cuts at: 0 812 1490 1733 1777 1796 1840 2295
Size: 812 678 243 44 19 44 455
Fragments arranged by size:
812 678 455 243 44 44 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 550 1294 2295
Size: 550 744 1001
Fragments arranged by size:
1001 744 550
BsrBI CCG'CTC
Cuts at: 0 1596 1721 2295
Size: 1596 125 574
Fragments arranged by size:
1596 574 125
BsrFI r'CCGG_y
Cuts at: 0 2095 2295
Size: 2095 200
BsrGI T'GTAC_A
Cuts at: 0 245 2295
Size: 245 2050
BstEII G'GTnAC_C
Cuts at: 0 1642 2170 2295
Size: 1642 528 125
Fragments arranged by size:
1642 528 125
BstXI CCAn_nnnn'nTGG
Cuts at: 0 828 1984 2295
Size: 828 1156 311
Fragments arranged by size:
1156 828 311
BstYI r'GATC_y
Cuts at: 0 230 261 1361 1697 2016 2295
Size: 230 31 1100 336 319 279
Fragments arranged by size:
1100 336 319 279 230 31
Bsu36I CC'TnA_GG
Cuts at: 0 2060 2295
Size: 2060 235
BtgI C'CryG_G
Cuts at: 0 749 2163 2295
Size: 749 1414 132
Fragments arranged by size:
1414 749 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2272 2295
Size: 2272 23
BtsI GCAGTG_nn'
Cuts at: 0 1752 2107 2295
Size: 1752 355 188
Fragments arranged by size:
1752 355 188
DraI TTT'AAA
Cuts at: 0 108 350 1212 2295
Size: 108 242 862 1083
Fragments arranged by size:
1083 862 242 108
DraIII CAC_nnn'GTG
Cuts at: 0 1908 2295
Size: 1908 387
DrdI GACnn_nn'nnGTC
Cuts at: 0 1167 2295
Size: 1167 1128
EaeI y'GGCC_r
Cuts at: 0 2126 2295
Size: 2126 169
EcoICRI GAG'CTC
Cuts at: 0 1794 1838 2295
Size: 1794 44 457
Fragments arranged by size:
1794 457 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 575 817 946 964 1079 1148 1150
Size: 19 556 242 129 18 115 69 2
Cuts at: 1150 1455 1807 2217 2295
Size: 305 352 410 78
Fragments arranged by size:
556 410 352 305 242 129 115 78
69 19 18 2
EcoRI G'AATT_C
Cuts at: 0 165 1577 2295
Size: 165 1412 718
Fragments arranged by size:
1412 718 165
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 85 2295
Size: 85 2210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 321 353 955 987 1834 1866 1924 1956
Size: 321 32 602 32 847 32 58 32
Cuts at: 1956 1965 1997 2001 2033 2043 2075 2295
Size: 9 32 4 32 10 32 220
Fragments arranged by size:
847 602 321 220 58 32 32 32
32 32 32 32 10 9 4
MluI A'CGCG_T
Cuts at: 0 1184 2295
Size: 1184 1111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 845 989 1841 2001 2295
Size: 488 357 144 852 160 294
Fragments arranged by size:
852 488 357 294 160 144
MscI TGG'CCA
Cuts at: 0 2128 2295
Size: 2128 167
MslI CAynn'nnrTG
Cuts at: 0 119 200 524 1253 1337 1437 1439 1864
Size: 119 81 324 729 84 100 2 425
Cuts at: 1864 2128 2168 2295
Size: 264 40 127
Fragments arranged by size:
729 425 324 264 127 119 100 84
81 40 2
MspA1I CmG'CkG
Cuts at: 0 23 602 685 1607 2295
Size: 23 579 83 922 688
Fragments arranged by size:
922 688 579 83 23
NheI G'CTAG_C
Cuts at: 0 1042 2253 2295
Size: 1042 1211 42
Fragments arranged by size:
1211 1042 42
NsiI A_TGCA'T
Cuts at: 0 793 2295
Size: 793 1502
NspI r_CATG'y
Cuts at: 0 384 529 881 1660 1694 2295
Size: 384 145 352 779 34 601
Fragments arranged by size:
779 601 384 352 145 34
PasI CC'CwG_GG
Cuts at: 0 2178 2295
Size: 2178 117
PciI A'CATG_T
Cuts at: 0 1690 2295
Size: 1690 605
PflMI CCAn_nnn'nTGG
Cuts at: 0 1908 2098 2295
Size: 1908 190 197
Fragments arranged by size:
1908 197 190
PfoI T'CCnGG_A
Cuts at: 0 233 794 2295
Size: 233 561 1501
Fragments arranged by size:
1501 561 233
PstI C_TGCA'G
Cuts at: 0 473 2114 2273 2295
Size: 473 1641 159 22
Fragments arranged by size:
1641 473 159 22
PvuII CAG'CTG
Cuts at: 0 23 602 685 1607 2295
Size: 23 579 83 922 688
Fragments arranged by size:
922 688 579 83 23
SacI G_AGCT'C
Cuts at: 0 1796 1840 2295
Size: 1796 44 455
Fragments arranged by size:
1796 455 44
SfcI C'TryA_G
Cuts at: 0 362 469 737 2110 2269 2295
Size: 362 107 268 1373 159 26
Fragments arranged by size:
1373 362 268 159 107 26
SmlI C'TyrA_G
Cuts at: 0 1822 2295
Size: 1822 473
SphI G_CATG'C
Cuts at: 0 384 529 2295
Size: 384 145 1766
Fragments arranged by size:
1766 384 145
StuI AGG'CCT
Cuts at: 0 65 1125 2065 2295
Size: 65 1060 940 230
Fragments arranged by size:
1060 940 230 65
StyI C'CwwG_G
Cuts at: 0 60 2295
Size: 60 2235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1232 1919 2295
Size: 1232 687 376
Fragments arranged by size:
1232 687 376
TatI w'GTAC_w
Cuts at: 0 245 366 394 2295
Size: 245 121 28 1901
Fragments arranged by size:
1901 245 121 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 802 834 2295
Size: 802 32 1461
Fragments arranged by size:
1461 802 32
XbaI T'CTAG_A
Cuts at: 0 258 2295
Size: 258 2037
XhoI C'TCGA_G
Cuts at: 0 1822 2295
Size: 1822 473
XmnI GAAnn'nnTTC
Cuts at: 0 1291 2295
Size: 1291 1004
ZraI GAC'GTC
Cuts at: 0 1395 2295
Size: 1395 900
Enzymes that do cut:
AarI AatII AclI AcuI AflIII AleI AlfI AlwNI
ApoI AvaI BamHI BanII BbsI BcgI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BpmI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrFI BsrGI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EcoICRI
Eco57MI EcoRI FalI Hin4I MluI MmeI MscI MslI
MspA1I NheI NsiI NspI PasI PciI PflMI PfoI
PstI PvuII SacI SfcI SmlI SphI StuI StyI
TaqII TatI TstI XbaI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AfeI AflII AgeI AhdI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI
BbvCI BciVI BclI Bme1580I BplI Bpu10I BpuEI BsaAI
BsaBI BsaWI BsiEI BsiWI BspEI BspHI BsrDI BssHII
BssSI BstAPI BstBI BstZ17I ClaI CspCI EagI EarI
EciI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI NaeI NarI
NcoI NdeI NgoMIV NotI NruI PacI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI Tth111I XcmI XmaI