Restriction map for
pFN21AE1180
(Linear) (Six-base) MAPSORT of: KIEE1180.seq Check: 3395 from: 1 to: 2091
KIEE1180.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AccI GT'mk_AC
Cuts at: 0 1264 1611 2091
Size: 1264 347 480
Fragments arranged by size:
1264 480 347
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 2091
Size: 208 1883
AleI CACnn'nnGTG
Cuts at: 0 1211 1213 2091
Size: 1211 2 878
Fragments arranged by size:
1211 878 2
AlwNI CAG_nnn'CTG
Cuts at: 0 72 2091
Size: 72 2019
ApaLI G'TGCA_C
Cuts at: 0 1456 2091
Size: 1456 635
ApoI r'AATT_y
Cuts at: 0 214 571 626 847 1202 1330 1428 1666
Size: 214 357 55 221 355 128 98 238
Cuts at: 1666 1706 1764 1874 2005 2042 2091
Size: 40 58 110 131 37 49
Fragments arranged by size:
357 355 238 221 214 131 128 110
98 58 55 49 40 37
BamHI G'GATC_C
Cuts at: 0 75 2091
Size: 75 2016
BbsI GAAGACnn'nnnn_
Cuts at: 0 1099 2091
Size: 1099 992
Bme1580I G_kGCm'C
Cuts at: 0 1460 2091
Size: 1460 631
BmgBI CAC'GTC
Cuts at: 0 36 2091
Size: 36 2055
BmrI ACTGGGnnnn_n'
Cuts at: 0 1503 2091
Size: 1503 588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 736 2091
Size: 133 603 1355
Fragments arranged by size:
1355 603 133
Bpu10I CC'TnA_GC
Cuts at: 0 80 2091
Size: 80 2011
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 2091
Size: 559 1532
BsaI GGTCTCn'nnnn_
Cuts at: 0 313 1128 2091
Size: 313 815 963
Fragments arranged by size:
963 815 313
BsaWI w'CCGG_w
Cuts at: 0 159 2091
Size: 159 1932
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 514 2091
Size: 514 1577
BsiHKAI G_wGCw'C
Cuts at: 0 1460 2091
Size: 1460 631
BsmI GAATG_Cn'
Cuts at: 0 667 814 1654 2023 2091
Size: 667 147 840 369 68
Fragments arranged by size:
840 667 369 147 68
Bsp1286I G_dGCh'C
Cuts at: 0 1460 2091
Size: 1460 631
BspHI T'CATG_A
Cuts at: 0 1149 1439 2091
Size: 1149 290 652
Fragments arranged by size:
1149 652 290
BsrGI T'GTAC_A
Cuts at: 0 771 1359 1947 2091
Size: 771 588 588 144
Fragments arranged by size:
771 588 588 144
BstYI r'GATC_y
Cuts at: 0 75 733 2091
Size: 75 658 1358
Fragments arranged by size:
1358 658 75
Bsu36I CC'TnA_GG
Cuts at: 0 1282 2091
Size: 1282 809
BtgI C'CryG_G
Cuts at: 0 196 2091
Size: 196 1895
BtsI GCAGTG_nn'
Cuts at: 0 819 2091
Size: 819 1272
EarI CTCTTCn'nnn_
Cuts at: 0 185 275 326 2091
Size: 185 90 51 1765
Fragments arranged by size:
1765 185 90 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 208 736 2091
Size: 133 75 528 1355
Fragments arranged by size:
1355 528 133 75
EcoO109I rG'GnC_Cy
Cuts at: 0 1286 2091
Size: 1286 805
EcoRI G'AATT_C
Cuts at: 0 214 1428 1706 1764 1874 2042 2091
Size: 214 1214 278 58 110 168 49
Fragments arranged by size:
1214 278 214 168 110 58 49
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 631 2091
Size: 631 1460
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1839 1871 2091
Size: 1839 32 220
Fragments arranged by size:
1839 220 32
HindIII A'AGCT_T
Cuts at: 0 535 563 2091
Size: 535 28 1528
Fragments arranged by size:
1528 535 28
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 740 2091
Size: 158 582 1351
Fragments arranged by size:
1351 582 158
MslI CAynn'nnrTG
Cuts at: 0 1154 1211 1213 2091
Size: 1154 57 2 878
Fragments arranged by size:
1154 878 57 2
MspA1I CmG'CkG
Cuts at: 0 72 2091
Size: 72 2019
NcoI C'CATG_G
Cuts at: 0 196 2091
Size: 196 1895
NdeI CA'TA_TG
Cuts at: 0 788 2091
Size: 788 1303
PasI CC'CwG_GG
Cuts at: 0 12 2091
Size: 12 2079
PfoI T'CCnGG_A
Cuts at: 0 275 552 2091
Size: 275 277 1539
Fragments arranged by size:
1539 277 275
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 329 361 2091
Size: 329 32 1730
Fragments arranged by size:
1730 329 32
PpuMI rG'GwC_Cy
Cuts at: 0 1286 2091
Size: 1286 805
PvuII CAG'CTG
Cuts at: 0 72 2091
Size: 72 2019
SapI GCTCTTCn'nnn_
Cuts at: 0 185 2091
Size: 185 1906
SfcI C'TryA_G
Cuts at: 0 120 2091
Size: 120 1971
SmlI C'TyrA_G
Cuts at: 0 538 2091
Size: 538 1553
StyI C'CwwG_G
Cuts at: 0 196 2091
Size: 196 1895
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 67 2091
Size: 67 2024
TatI w'GTAC_w
Cuts at: 0 771 1359 1947 1987 2091
Size: 771 588 588 40 104
Fragments arranged by size:
771 588 588 104 40
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 242 2091
Size: 242 1849
XmnI GAAnn'nnTTC
Cuts at: 0 673 829 913 1122 1165 1249 1374 1542
Size: 673 156 84 209 43 84 125 168
Cuts at: 1542 1585 1710 1753 1837 1878 1962 2005 2091
Size: 43 125 43 84 41 84 43 86
Fragments arranged by size:
673 209 168 156 125 125 86 84
84 84 84 43 43 43 43 41
Enzymes that do cut:
AccI AcuI AleI AlwNI ApaLI ApoI BamHI BbsI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaWI
BseRI BsiHKAI BsmI Bsp1286I BspHI BsrGI BstYI Bsu36I
BtgI BtsI EarI Eco57MI EcoO109I EcoRI FalI Hin4I
HindIII MmeI MslI MspA1I NcoI NdeI PasI PfoI
PpiI PpuMI PvuII SapI SfcI SmlI StyI TaqII
TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmtI BplI BsaAI
BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiWI BsmBI
BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI
ZraI