Restriction map for
pFN21AE1179
(Linear) (Six-base) MAPSORT of: KIEE1179.seq Check: 9151 from: 1 to: 2067
KIEE1179.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AccI GT'mk_AC
Cuts at: 0 1950 2067
Size: 1950 117
Acc65I G'GTAC_C
Cuts at: 0 1694 2067
Size: 1694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1609 2067
Size: 1609 458
AlwNI CAG_nnn'CTG
Cuts at: 0 495 2047 2067
Size: 495 1552 20
Fragments arranged by size:
1552 495 20
ApaI G_GGCC'C
Cuts at: 0 100 164 343 449 725 742 904 1066
Size: 100 64 179 106 276 17 162 162
Cuts at: 1066 1219 1237 1256 1310 1391 1688 1714 1787
Size: 153 18 19 54 81 297 26 73
Cuts at: 1787 1856 2067
Size: 69 211
Fragments arranged by size:
297 276 211 179 162 162 153 106
100 81 73 69 64 54 26 19
18 17
ApoI r'AATT_y
Cuts at: 0 1244 2067
Size: 1244 823
AvaI C'yCGr_G
Cuts at: 0 102 111 238 310 477 1121 1490 1528
Size: 102 9 127 72 167 644 369 38
Cuts at: 1528 1733 1840 2067
Size: 205 107 227
Fragments arranged by size:
644 369 227 205 167 127 107 102
72 38 9
AvrII C'CTAG_G
Cuts at: 0 805 2067
Size: 805 1262
BamHI G'GATC_C
Cuts at: 0 133 287 1862 2050 2067
Size: 133 154 1575 188 17
Fragments arranged by size:
1575 188 154 133 17
BanI G'GyrC_C
Cuts at: 0 649 775 1450 1694 1721 1835 2067
Size: 649 126 675 244 27 114 232
Fragments arranged by size:
675 649 244 232 126 114 27
BanII G_rGCy'C
Cuts at: 0 100 164 343 449 725 742 904 1066
Size: 100 64 179 106 276 17 162 162
Cuts at: 1066 1219 1237 1256 1291 1310 1391 1688 1714
Size: 153 18 19 35 19 81 297 26
Cuts at: 1714 1787 1856 1935 2067
Size: 73 69 79 132
Fragments arranged by size:
297 276 179 162 162 153 132 106
100 81 79 73 69 64 35 26
19 19 18 17
BbeI G_GCGC'C
Cuts at: 0 1454 2067
Size: 1454 613
BbsI GAAGACnn'nnnn_
Cuts at: 0 1306 2067
Size: 1306 761
BglI GCCn_nnn'nGGC
Cuts at: 0 13 222 338 908 998 1070 1736 1886
Size: 13 209 116 570 90 72 666 150
Cuts at: 1886 1964 2067
Size: 78 103
Fragments arranged by size:
666 570 209 150 116 103 90 78
72 13
Bme1580I G_kGCm'C
Cuts at: 0 100 164 343 449 725 742 904 1066
Size: 100 64 179 106 276 17 162 162
Cuts at: 1066 1219 1237 1256 1310 1391 1688 1714 1724
Size: 153 18 19 54 81 297 26 10
Cuts at: 1724 1787 1838 1856 2067
Size: 63 51 18 211
Fragments arranged by size:
297 276 211 179 162 162 153 106
100 81 64 63 54 51 26 19
18 18 17 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 791 2067
Size: 791 1276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 278 314 677 1064 1655 1919 2067
Size: 28 250 36 363 387 591 264 148
Fragments arranged by size:
591 387 363 264 250 148 36 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 1040 2067
Size: 1040 1027
BsaAI yAC'GTr
Cuts at: 0 1411 2067
Size: 1411 656
BsaBI GATnn'nnATC
Cuts at: 0 400 1913 2067
Size: 400 1513 154
Fragments arranged by size:
1513 400 154
BsaHI Gr'CG_yC
Cuts at: 0 1271 1451 2067
Size: 1271 180 616
Fragments arranged by size:
1271 616 180
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1273 1303 2067
Size: 1273 30 764
Fragments arranged by size:
1273 764 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 1139 2067
Size: 31 1108 928
Fragments arranged by size:
1108 928 31
BseYI C'CCAG_C
Cuts at: 0 211 826 1435 2067
Size: 211 615 609 632
Fragments arranged by size:
632 615 609 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 980 2067
Size: 980 1087
BsiHKAI G_wGCw'C
Cuts at: 0 59 2067
Size: 59 2008
BsmBI CGTCTCn'nnnn_
Cuts at: 0 727 2067
Size: 727 1340
Bsp1286I G_dGCh'C
Cuts at: 0 59 100 164 343 449 725 742 904
Size: 59 41 64 179 106 276 17 162
Cuts at: 904 1066 1219 1237 1256 1291 1310 1391 1688
Size: 162 153 18 19 35 19 81 297
Cuts at: 1688 1714 1724 1787 1838 1856 1935 2067
Size: 26 10 63 51 18 79 132
Fragments arranged by size:
297 276 179 162 162 153 132 106
81 79 64 63 59 51 41 35
26 19 19 18 18 17 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1936 2004 2067
Size: 1936 68 63
Fragments arranged by size:
1936 68 63
BsrBI CCG'CTC
Cuts at: 0 93 2067
Size: 93 1974
BsrFI r'CCGG_y
Cuts at: 0 1339 1880 2067
Size: 1339 541 187
Fragments arranged by size:
1339 541 187
BstEII G'GTnAC_C
Cuts at: 0 1162 1324 2067
Size: 1162 162 743
Fragments arranged by size:
1162 743 162
BstYI r'GATC_y
Cuts at: 0 133 287 1862 2050 2067
Size: 133 154 1575 188 17
Fragments arranged by size:
1575 188 154 133 17
Bsu36I CC'TnA_GG
Cuts at: 0 1202 1220 2067
Size: 1202 18 847
Fragments arranged by size:
1202 847 18
BtgI C'CryG_G
Cuts at: 0 281 1311 1716 1760 1970 2067
Size: 281 1030 405 44 210 97
Fragments arranged by size:
1030 405 281 210 97 44
BtsI GCAGTG_nn'
Cuts at: 0 510 963 2067
Size: 510 453 1104
Fragments arranged by size:
1104 510 453
DraIII CAC_nnn'GTG
Cuts at: 0 793 1575 2067
Size: 793 782 492
Fragments arranged by size:
793 782 492
DrdI GACnn_nn'nnGTC
Cuts at: 0 683 2067
Size: 683 1384
EaeI y'GGCC_r
Cuts at: 0 2 612 1337 1499 1878 1887 2067
Size: 2 610 725 162 379 9 180
Fragments arranged by size:
725 610 379 180 162 9 2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 278 314 677 1064 1609 1655 1919
Size: 28 250 36 363 387 545 46 264
Cuts at: 1919 2067
Size: 148
Fragments arranged by size:
545 387 363 264 250 148 46 36
28
EcoO109I rG'GnC_Cy
Cuts at: 0 96 97 106 161 187 340 409 420
Size: 96 1 9 55 26 153 69 11
Cuts at: 420 445 446 454 550 631 685 721 739
Size: 25 1 8 96 81 54 36 18
Cuts at: 739 802 901 929 1039 1071 1117 1198 1215
Size: 63 99 28 110 32 46 81 17
Cuts at: 1215 1216 1233 1234 1252 1253 1307 1387 1666
Size: 1 17 1 18 1 54 80 279
Cuts at: 1666 1675 1685 1710 1711 1783 1853 1922 1966
Size: 9 10 25 1 72 70 69 44
Cuts at: 1966 2062 2067
Size: 96 5
Fragments arranged by size:
279 153 110 99 96 96 96 81
81 80 72 70 69 69 63 55
54 54 46 44 36 32 28 26
25 25 18 18 17 17 11 10
9 9 8 5 1 1 1 1
1 1
EcoRI G'AATT_C
Cuts at: 0 1244 2067
Size: 1244 823
FseI GG_CCGG'CC
Cuts at: 0 1343 1884 2067
Size: 1343 541 183
Fragments arranged by size:
1343 541 183
HaeII r_GCGC'y
Cuts at: 0 770 1454 1588 2067
Size: 770 684 134 479
Fragments arranged by size:
770 684 479 134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 63 95 424 456 726 758 1273 1305
Size: 63 32 329 32 270 32 515 32
Cuts at: 1305 1653 1685 1906 1938 2035 2067
Size: 348 32 221 32 97 32
Fragments arranged by size:
515 348 329 270 221 97 63 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1951 2067
Size: 1951 116
KasI G'GCGC_C
Cuts at: 0 1450 2067
Size: 1450 617
KpnI G_GTAC'C
Cuts at: 0 1698 2067
Size: 1698 369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 547 2067
Size: 161 386 1520
Fragments arranged by size:
1520 386 161
MscI TGG'CCA
Cuts at: 0 614 1889 2067
Size: 614 1275 178
Fragments arranged by size:
1275 614 178
MspA1I CmG'CkG
Cuts at: 0 1313 1439 2067
Size: 1313 126 628
Fragments arranged by size:
1313 628 126
NaeI GCC'GGC
Cuts at: 0 1341 1882 2067
Size: 1341 541 185
Fragments arranged by size:
1341 541 185
NarI GG'CG_CC
Cuts at: 0 1451 2067
Size: 1451 616
NcoI C'CATG_G
Cuts at: 0 281 1716 2067
Size: 281 1435 351
Fragments arranged by size:
1435 351 281
NgoMIV G'CCGG_C
Cuts at: 0 1339 1880 2067
Size: 1339 541 187
Fragments arranged by size:
1339 541 187
NspI r_CATG'y
Cuts at: 0 583 2067
Size: 583 1484
PasI CC'CwG_GG
Cuts at: 0 460 556 690 870 1096 1140 1294 1330
Size: 460 96 134 180 226 44 154 36
Cuts at: 1330 1456 1644 1690 1858 1927 2067
Size: 126 188 46 168 69 140
Fragments arranged by size:
460 226 188 180 168 154 140 134
126 96 69 46 44 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 495 2067
Size: 495 1572
PfoI T'CCnGG_A
Cuts at: 0 183 369 1077 1671 1815 1935 2067
Size: 183 186 708 594 144 120 132
Fragments arranged by size:
708 594 186 183 144 132 120
PpuMI rG'GwC_Cy
Cuts at: 0 106 187 420 454 550 631 685 802
Size: 106 81 233 34 96 81 54 117
Cuts at: 802 929 1666 1675 1922 2067
Size: 127 737 9 247 145
Fragments arranged by size:
737 247 233 145 127 117 106 96
81 81 54 34 9
PspOMI G'GGCC_C
Cuts at: 0 96 160 339 445 721 738 900 1062
Size: 96 64 179 106 276 17 162 162
Cuts at: 1062 1215 1233 1252 1306 1387 1684 1710 1783
Size: 153 18 19 54 81 297 26 73
Cuts at: 1783 1852 2067
Size: 69 215
Fragments arranged by size:
297 276 215 179 162 162 153 106
96 81 73 69 64 54 26 19
18 17
PspXI vC'TCGA_Gb
Cuts at: 0 1121 1490 2067
Size: 1121 369 577
Fragments arranged by size:
1121 577 369
PstI C_TGCA'G
Cuts at: 0 566 2067
Size: 566 1501
SacII CC_GC'GG
Cuts at: 0 1314 2067
Size: 1314 753
SalI G'TCGA_C
Cuts at: 0 1949 2067
Size: 1949 118
SanDI GG'GwC_CC
Cuts at: 0 106 187 420 631 685 929 1922 2067
Size: 106 81 233 211 54 244 993 145
Fragments arranged by size:
993 244 233 211 145 106 81 54
SexAI A'CCwGG_T
Cuts at: 0 468 2067
Size: 468 1599
SfcI C'TryA_G
Cuts at: 0 562 2067
Size: 562 1505
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 222 338 1070 1886 1964 2067
Size: 222 116 732 816 78 103
Fragments arranged by size:
816 732 222 116 103 78
SfoI GGC'GCC
Cuts at: 0 1452 2067
Size: 1452 615
SmaI CCC'GGG
Cuts at: 0 104 113 240 312 479 1530 1842 2067
Size: 104 9 127 72 167 1051 312 225
Fragments arranged by size:
1051 312 225 167 127 104 72 9
SmlI C'TyrA_G
Cuts at: 0 1121 1490 2067
Size: 1121 369 577
Fragments arranged by size:
1121 577 369
SphI G_CATG'C
Cuts at: 0 583 2067
Size: 583 1484
SrfI GCCC'GGGC
Cuts at: 0 312 2067
Size: 312 1755
StyI C'CwwG_G
Cuts at: 0 191 263 281 413 635 725 805 1167
Size: 191 72 18 132 222 90 80 362
Cuts at: 1167 1257 1391 1716 1787 2007 2067
Size: 90 134 325 71 220 60
Fragments arranged by size:
362 325 222 220 191 134 132 90
90 80 72 71 60 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 592 637 1312 1636 2067
Size: 592 45 675 324 431
Fragments arranged by size:
675 592 431 324 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 502 534 588 620 2067
Size: 502 32 54 32 1447
Fragments arranged by size:
1447 502 54 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 492 642 1723 2067
Size: 492 150 1081 344
Fragments arranged by size:
1081 492 344 150
XhoI C'TCGA_G
Cuts at: 0 1121 1490 2067
Size: 1121 369 577
Fragments arranged by size:
1121 577 369
XmaI C'CCGG_G
Cuts at: 0 102 111 238 310 477 1528 1840 2067
Size: 102 9 127 72 167 1051 312 227
Fragments arranged by size:
1051 312 227 167 127 102 72 9
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApaI ApoI AvaI AvrII
BamHI BanI BanII BbeI BbsI BglI Bme1580I BmrI
BpmI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BstEII
BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI Eco57MI
EcoO109I EcoRI FseI HaeII Hin4I HincII KasI KpnI
MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PfoI PpuMI PspOMI PspXI PstI SacII
SalI SanDI SexAI SfcI SfiI SfoI SmaI SmlI
SphI SrfI StyI TaqII TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI BaeI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaWI BsiEI BsiWI BsmI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstZ17I BtgZI ClaI CspCI DraI EagI EarI
EciI EcoICRI EcoNI EcoRV FalI FspI FspAI HindIII
HpaI MfeI MluI MslI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI PvuII RsrII SacI SapI SbfI ScaI
SgfI SgrAI SnaBI SpeI SspI StuI SwaI TatI
Tth111I XbaI XmnI ZraI