(Linear) (Six-base) MAPSORT of: KIEE1175.seq  Check: 6224  from: 1  to: 1992



KIEE1175.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1387    1992 
   Size:       1387     605
 
AatII G_ACGT'C

Cuts at:      0    1814    1992 
   Size:       1814     178
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     216    1964    1992 
   Size:        216    1748      28

  Fragments arranged by size: 

                1748     216      28
 
AflII C'TTAA_G

Cuts at:      0     169    1992 
   Size:        169    1823
 
AgeI A'CCGG_T

Cuts at:      0     543    1992 
   Size:        543    1449
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1095    1992 
   Size:       1095     897
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1089    1121    1992 
   Size:       1089      32     871

  Fragments arranged by size: 

                1089     871      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1243    1992 
   Size:       1243     749
 
ApoI r'AATT_y

Cuts at:      0     246     265     712    1549    1992 
   Size:        246      19     447     837     443

  Fragments arranged by size: 

                 837     447     443     246      19
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     545     578    1992 
   Size:        545      33    1414

  Fragments arranged by size: 

                1414     545      33
 
BanII G_rGCy'C

Cuts at:      0     578    1992 
   Size:        578    1414
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1034    1992 
   Size:       1034     958
 
BglII A'GATC_T

Cuts at:      0    1975    1992 
   Size:       1975      17
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     271    1992 
   Size:         98     173    1721

  Fragments arranged by size: 

                1721     173      98
 
BsaHI Gr'CG_yC

Cuts at:      0    1811    1992 
   Size:       1811     181
 
BsaWI w'CCGG_w

Cuts at:      0     543    1992 
   Size:        543    1449
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     269     299    1260    1290    1992 
   Size:        269      30     961      30     702

  Fragments arranged by size: 

                 961     702     269      30      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     578    1992 
   Size:        578    1414
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1387    1992 
   Size:       1387     605
 
BsrFI r'CCGG_y

Cuts at:      0     543    1329    1992 
   Size:        543     786     663

  Fragments arranged by size: 

                 786     663     543
 
BstBI TT'CG_AA

Cuts at:      0     747    1992 
   Size:        747    1245
 
BstEII G'GTnAC_C

Cuts at:      0     679    1992 
   Size:        679    1313
 
BstYI r'GATC_y

Cuts at:      0      81    1975    1992 
   Size:         81    1894      17

  Fragments arranged by size: 

                1894      81      17
 
Bsu36I CC'TnA_GG

Cuts at:      0     632    1992 
   Size:        632    1360
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1848    1883    1992 
   Size:       1848      35     109

  Fragments arranged by size: 

                1848     109      35
 
EaeI y'GGCC_r

Cuts at:      0    1401    1992 
   Size:       1401     591
 
EarI CTCTTCn'nnn_

Cuts at:      0     235    1853    1992 
   Size:        235    1618     139

  Fragments arranged by size: 

                1618     235     139
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     216     271    1964    1992 
   Size:         98     118      55    1693      28

  Fragments arranged by size: 

                1693     118      98      55      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1186    1726    1992 
   Size:       1186     540     266

  Fragments arranged by size: 

                1186     540     266
 
EcoRI G'AATT_C

Cuts at:      0     712    1992 
   Size:        712    1280
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1745    1992 
   Size:       1745     247
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     438     470     681     713    1992 
   Size:        438      32     211      32    1279

  Fragments arranged by size: 

                1279     438     211      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1567    1992 
   Size:       1567     425
 
MscI TGG'CCA

Cuts at:      0    1403    1992 
   Size:       1403     589
 
MslI CAynn'nnrTG

Cuts at:      0     917    1077    1310    1526    1992 
   Size:        917     160     233     216     466

  Fragments arranged by size: 

                 917     466     233     216     160
 
MspA1I CmG'CkG

Cuts at:      0    1435    1992 
   Size:       1435     557
 
NdeI CA'TA_TG

Cuts at:      0    1130    1992 
   Size:       1130     862
 
NspI r_CATG'y

Cuts at:      0    1076    1992 
   Size:       1076     916
 
PfoI T'CCnGG_A

Cuts at:      0      68     992    1992 
   Size:         68     924    1000

  Fragments arranged by size: 

                1000     924      68
 
PstI C_TGCA'G

Cuts at:      0    1398    1992 
   Size:       1398     594
 
PvuII CAG'CTG

Cuts at:      0    1435    1992 
   Size:       1435     557
 
ScaI AGT'ACT

Cuts at:      0     483    1992 
   Size:        483    1509
 
SfcI C'TryA_G

Cuts at:      0     106     569    1244    1394    1992 
   Size:        106     463     675     150     598

  Fragments arranged by size: 

                 675     598     463     150     106
 
SmlI C'TyrA_G

Cuts at:      0     169    1992 
   Size:        169    1823
 
SpeI A'CTAG_T

Cuts at:      0    1381    1992 
   Size:       1381     611
 
SphI G_CATG'C

Cuts at:      0    1076    1992 
   Size:       1076     916
 
TatI w'GTAC_w

Cuts at:      0     481     753     785    1969    1992 
   Size:        481     272      32    1184      23

  Fragments arranged by size: 

                1184     481     272      32      23
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     269     301    1992 
   Size:        269      32    1691

  Fragments arranged by size: 

                1691     269      32
 
XbaI T'CTAG_A

Cuts at:      0     721    1992 
   Size:        721    1271
 
ZraI GAC'GTC

Cuts at:      0    1812    1992 
   Size:       1812     180
 

 Enzymes that do cut:

     AarI    AatII     AcuI    AflII     AgeI     AlfI     AloI    AlwNI
     ApoI     BaeI    BanII     BbsI    BglII     BpmI    BsaHI    BsaWI
    BsaXI Bsp1286I    BspMI    BsrFI    BstBI   BstEII    BstYI   Bsu36I
    CspCI     EaeI     EarI  Eco57MI EcoO109I    EcoRI     FalI    Hin4I
     MmeI     MscI     MslI   MspA1I     NdeI     NspI     PfoI     PstI
    PvuII     ScaI     SfcI     SmlI     SpeI     SphI     TatI     TstI
     XbaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI   AflIII     AhdI     AleI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI
     SspI     StuI     StyI     SwaI    TaqII  Tth111I     XcmI     XhoI
     XmaI     XmnI