Restriction map for
pFN21AE1174
(Linear) (Six-base) MAPSORT of: KIEE1174.seq Check: 6066 from: 1 to: 1905
KIEE1174.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 465 1057 1218 1905
Size: 465 592 161 687
Fragments arranged by size:
687 592 465 161
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1402 1905
Size: 1402 503
AgeI A'CCGG_T
Cuts at: 0 956 1905
Size: 956 949
AlwNI CAG_nnn'CTG
Cuts at: 0 75 186 1905
Size: 75 111 1719
Fragments arranged by size:
1719 111 75
ApaI G_GGCC'C
Cuts at: 0 194 267 355 912 1157 1448 1815 1905
Size: 194 73 88 557 245 291 367 90
Fragments arranged by size:
557 367 291 245 194 90 88 73
AvaI C'yCGr_G
Cuts at: 0 283 814 977 983 1085 1150 1273 1846
Size: 283 531 163 6 102 65 123 573
Cuts at: 1846 1905
Size: 59
Fragments arranged by size:
573 531 283 163 123 102 65 59
6
BanI G'GyrC_C
Cuts at: 0 334 477 496 559 902 1793 1856 1905
Size: 334 143 19 63 343 891 63 49
Fragments arranged by size:
891 343 334 143 63 63 49 19
BanII G_rGCy'C
Cuts at: 0 194 267 355 912 984 1157 1448 1726
Size: 194 73 88 557 72 173 291 278
Cuts at: 1726 1815 1905
Size: 89 90
Fragments arranged by size:
557 291 278 194 173 90 89 88
73 72
BbeI G_GCGC'C
Cuts at: 0 500 563 1797 1905
Size: 500 63 1234 108
Fragments arranged by size:
1234 500 108 63
BbsI GAAGACnn'nnnn_
Cuts at: 0 1587 1905
Size: 1587 318
BbvCI CC'TCA_GC
Cuts at: 0 379 1905
Size: 379 1526
BglI GCCn_nnn'nGGC
Cuts at: 0 350 622 791 1528 1905
Size: 350 272 169 737 377
Fragments arranged by size:
737 377 350 272 169
BlpI GC'TnA_GC
Cuts at: 0 88 685 1657 1905
Size: 88 597 972 248
Fragments arranged by size:
972 597 248 88
Bme1580I G_kGCm'C
Cuts at: 0 105 194 267 337 355 482 815 912
Size: 105 89 73 70 18 127 333 97
Cuts at: 912 1157 1448 1485 1815 1905
Size: 245 291 37 330 90
Fragments arranged by size:
333 330 291 245 127 105 97 90
89 73 70 37 18
BmgBI CAC'GTC
Cuts at: 0 1665 1905
Size: 1665 240
BmrI ACTGGGnnnn_n'
Cuts at: 0 948 1285 1905
Size: 948 337 620
Fragments arranged by size:
948 620 337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 1228 1347 1905
Size: 460 768 119 558
Fragments arranged by size:
768 558 460 119
Bpu10I CC'TnA_GC
Cuts at: 0 379 1905
Size: 379 1526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1636 1905
Size: 1636 269
BsaI GGTCTCn'nnnn_
Cuts at: 0 1327 1905
Size: 1327 578
BsaAI yAC'GTr
Cuts at: 0 1536 1905
Size: 1536 369
BsaHI Gr'CG_yC
Cuts at: 0 497 560 1794 1905
Size: 497 63 1234 111
Fragments arranged by size:
1234 497 111 63
BsaWI w'CCGG_w
Cuts at: 0 627 956 1346 1905
Size: 627 329 390 559
Fragments arranged by size:
627 559 390 329
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 586 609 616 639 1905
Size: 586 23 7 23 1266
Fragments arranged by size:
1266 586 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 963 1817 1905
Size: 963 854 88
Fragments arranged by size:
963 854 88
BseYI C'CCAG_C
Cuts at: 0 330 393 569 1027 1036 1265 1356 1441
Size: 330 63 176 458 9 229 91 85
Cuts at: 1441 1473 1539 1690 1905
Size: 32 66 151 215
Fragments arranged by size:
458 330 229 215 176 151 91 85
66 63 32 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 691 1246 1905
Size: 496 195 555 659
Fragments arranged by size:
659 555 496 195
BsiEI CG_ry'CG
Cuts at: 0 44 751 795 957 1439 1738 1905
Size: 44 707 44 162 482 299 167
Fragments arranged by size:
707 482 299 167 162 44 44
Bsp1286I G_dGCh'C
Cuts at: 0 105 194 267 337 355 482 815 912
Size: 105 89 73 70 18 127 333 97
Cuts at: 912 984 1157 1448 1485 1726 1815 1905
Size: 72 173 291 37 241 89 90
Fragments arranged by size:
333 291 241 173 127 105 97 90
89 89 73 72 70 37 18
BspEI T'CCGG_A
Cuts at: 0 627 1905
Size: 627 1278
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 465 1057 1218 1905
Size: 465 592 161 687
Fragments arranged by size:
687 592 465 161
BsrBI CCG'CTC
Cuts at: 0 12 40 1177 1884 1905
Size: 12 28 1137 707 21
Fragments arranged by size:
1137 707 28 21 12
BsrDI GCAATG_nn'
Cuts at: 0 249 845 1905
Size: 249 596 1060
Fragments arranged by size:
1060 596 249
BsrFI r'CCGG_y
Cuts at: 0 140 487 790 956 1021 1434 1500 1905
Size: 140 347 303 166 65 413 66 405
Fragments arranged by size:
413 405 347 303 166 140 66 65
BssSI C'ACGA_G
Cuts at: 0 644 1905
Size: 644 1261
BstEII G'GTnAC_C
Cuts at: 0 916 1017 1239 1905
Size: 916 101 222 666
Fragments arranged by size:
916 666 222 101
BstXI CCAn_nnnn'nTGG
Cuts at: 0 612 1473 1681 1797 1905
Size: 612 861 208 116 108
Fragments arranged by size:
861 612 208 116 108
Bsu36I CC'TnA_GG
Cuts at: 0 1251 1905
Size: 1251 654
BtgI C'CryG_G
Cuts at: 0 1116 1556 1562 1717 1905
Size: 1116 440 6 155 188
Fragments arranged by size:
1116 440 188 155 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 387 1038 1149 1905
Size: 387 651 111 756
Fragments arranged by size:
756 651 387 111
BtsI GCAGTG_nn'
Cuts at: 0 547 708 815 1137 1181 1905
Size: 547 161 107 322 44 724
Fragments arranged by size:
724 547 322 161 107 44
DraIII CAC_nnn'GTG
Cuts at: 0 1830 1905
Size: 1830 75
DrdI GACnn_nn'nnGTC
Cuts at: 0 1209 1905
Size: 1209 696
EaeI y'GGCC_r
Cuts at: 0 29 41 614 748 788 792 1279 1436
Size: 29 12 573 134 40 4 487 157
Cuts at: 1436 1460 1559 1638 1735 1905
Size: 24 99 79 97 170
Fragments arranged by size:
573 487 170 157 134 99 97 79
40 29 24 12 4
EagI C'GGCC_G
Cuts at: 0 41 748 792 1436 1735 1905
Size: 41 707 44 644 299 170
Fragments arranged by size:
707 644 299 170 44 41
EarI CTCTTCn'nnn_
Cuts at: 0 1886 1905
Size: 1886 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 1228 1347 1402 1905
Size: 460 768 119 55 503
Fragments arranged by size:
768 503 460 119 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 472 1905
Size: 472 1433
EcoO109I rG'GnC_Cy
Cuts at: 0 183 190 191 263 279 351 352 444
Size: 183 7 1 72 16 72 1 92
Cuts at: 444 909 1001 1154 1270 1445 1547 1687 1905
Size: 465 92 153 116 175 102 140 218
Fragments arranged by size:
465 218 183 175 153 140 116 102
92 92 72 72 16 7 1 1
FseI GG_CCGG'CC
Cuts at: 0 794 1905
Size: 794 1111
HaeII r_GCGC'y
Cuts at: 0 500 563 867 1028 1403 1797 1880 1905
Size: 500 63 304 161 375 394 83 25
Fragments arranged by size:
500 394 375 304 161 83 63 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 222 254 586 618 1254 1286 1905
Size: 222 32 332 32 636 32 619
Fragments arranged by size:
636 619 332 222 32 32 32
KasI G'GCGC_C
Cuts at: 0 496 559 1793 1905
Size: 496 63 1234 112
Fragments arranged by size:
1234 496 112 63
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 1905
Size: 629 1276
MscI TGG'CCA
Cuts at: 0 31 616 1905
Size: 31 585 1289
Fragments arranged by size:
1289 585 31
MspA1I CmG'CkG
Cuts at: 0 54 383 393 888 1441 1719 1748 1905
Size: 54 329 10 495 553 278 29 157
Fragments arranged by size:
553 495 329 278 157 54 29 10
NaeI GCC'GGC
Cuts at: 0 489 792 1502 1905
Size: 489 303 710 403
Fragments arranged by size:
710 489 403 303
NarI GG'CG_CC
Cuts at: 0 497 560 1794 1905
Size: 497 63 1234 111
Fragments arranged by size:
1234 497 111 63
NcoI C'CATG_G
Cuts at: 0 1116 1905
Size: 1116 789
NgoMIV G'CCGG_C
Cuts at: 0 487 790 1500 1905
Size: 487 303 710 405
Fragments arranged by size:
710 487 405 303
NotI GC'GGCC_GC
Cuts at: 0 41 1905
Size: 41 1864
PasI CC'CwG_GG
Cuts at: 0 186 482 912 1905
Size: 186 296 430 993
Fragments arranged by size:
993 430 296 186
PflMI CCAn_nnn'nTGG
Cuts at: 0 186 938 1539 1905
Size: 186 752 601 366
Fragments arranged by size:
752 601 366 186
PmlI CAC'GTG
Cuts at: 0 1536 1905
Size: 1536 369
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 279 311 808 840 1905
Size: 279 32 497 32 1065
Fragments arranged by size:
1065 497 279 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 279 444 1001 1270 1547 1905
Size: 279 165 557 269 277 358
Fragments arranged by size:
557 358 279 277 269 165
PspOMI G'GGCC_C
Cuts at: 0 190 263 351 908 1153 1444 1811 1905
Size: 190 73 88 557 245 291 367 94
Fragments arranged by size:
557 367 291 245 190 94 88 73
PstI C_TGCA'G
Cuts at: 0 368 1529 1905
Size: 368 1161 376
Fragments arranged by size:
1161 376 368
PvuII CAG'CTG
Cuts at: 0 393 1748 1905
Size: 393 1355 157
Fragments arranged by size:
1355 393 157
SacII CC_GC'GG
Cuts at: 0 1720 1905
Size: 1720 185
SanDI GG'GwC_CC
Cuts at: 0 1270 1547 1905
Size: 1270 277 358
Fragments arranged by size:
1270 358 277
SbfI CC_TGCA'GG
Cuts at: 0 1529 1905
Size: 1529 376
SfcI C'TryA_G
Cuts at: 0 364 1525 1905
Size: 364 1161 380
Fragments arranged by size:
1161 380 364
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 791 1905
Size: 791 1114
SfoI GGC'GCC
Cuts at: 0 498 561 1795 1905
Size: 498 63 1234 110
Fragments arranged by size:
1234 498 110 63
SmaI CCC'GGG
Cuts at: 0 985 1152 1275 1905
Size: 985 167 123 630
Fragments arranged by size:
985 630 167 123
SmlI C'TyrA_G
Cuts at: 0 283 1651 1905
Size: 283 1368 254
Fragments arranged by size:
1368 283 254
SrfI GCCC'GGGC
Cuts at: 0 985 1905
Size: 985 920
StuI AGG'CCT
Cuts at: 0 113 664 1905
Size: 113 551 1241
Fragments arranged by size:
1241 551 113
StyI C'CwwG_G
Cuts at: 0 1116 1703 1905
Size: 1116 587 202
Fragments arranged by size:
1116 587 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 565 861 904 934 1716 1905
Size: 565 296 43 30 782 189
Fragments arranged by size:
782 565 296 189 43 30
TatI w'GTAC_w
Cuts at: 0 1313 1905
Size: 1313 592
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 609 641 1116 1148 1654 1686 1905
Size: 609 32 475 32 506 32 219
Fragments arranged by size:
609 506 475 219 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 234 1905
Size: 234 1671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1254 1470 1831 1905
Size: 1254 216 361 74
Fragments arranged by size:
1254 361 216 74
XhoI C'TCGA_G
Cuts at: 0 283 1905
Size: 283 1622
XmaI C'CCGG_G
Cuts at: 0 983 1150 1273 1905
Size: 983 167 123 632
Fragments arranged by size:
983 632 167 123
Enzymes that do cut:
AarI AcuI AgeI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssSI BstEII BstXI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI Eco57MI EcoNI
EcoO109I FseI HaeII Hin4I KasI MmeI MscI MspA1I
NaeI NarI NcoI NgoMIV NotI PasI PflMI PmlI
PpiI PpuMI PspOMI PstI PvuII SacII SanDI SbfI
SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII
BmtI BplI BsaBI BsiHKAI BsiWI BsmI BsmBI BspHI
BsrGI BssHII BstAPI BstBI BstYI BstZ17I ClaI CspCI
DraI EciI EcoICRI EcoRI EcoRV FalI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MslI NdeI
NheI NruI NsiI NspI PacI PciI PfoI PmeI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SapI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI XbaI XmnI ZraI