Restriction map for
pFN21AE1173
(Linear) (Six-base) MAPSORT of: KIEE1173.seq Check: 3974 from: 1 to: 1818
KIEE1173.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 674 1011 1818
Size: 161 513 337 807
Fragments arranged by size:
807 513 337 161
AfeI AGC'GCT
Cuts at: 0 358 1818
Size: 358 1460
AflIII A'CryG_T
Cuts at: 0 887 1787 1818
Size: 887 900 31
Fragments arranged by size:
900 887 31
AlwNI CAG_nnn'CTG
Cuts at: 0 1551 1818
Size: 1551 267
ApoI r'AATT_y
Cuts at: 0 103 927 1326 1603 1818
Size: 103 824 399 277 215
Fragments arranged by size:
824 399 277 215 103
AseI AT'TA_AT
Cuts at: 0 1193 1818
Size: 1193 625
AvaI C'yCGr_G
Cuts at: 0 1252 1818
Size: 1252 566
BamHI G'GATC_C
Cuts at: 0 711 1818
Size: 711 1107
BanII G_rGCy'C
Cuts at: 0 15 783 1818
Size: 15 768 1035
Fragments arranged by size:
1035 768 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 559 1818
Size: 559 1259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 304 338 1818
Size: 304 34 1480
Fragments arranged by size:
1480 304 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1435 1818
Size: 1435 383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 1570 1726 1818
Size: 152 1418 156 92
Fragments arranged by size:
1418 156 152 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 1818
Size: 476 1342
BsaBI GATnn'nnATC
Cuts at: 0 1262 1818
Size: 1262 556
BsaWI w'CCGG_w
Cuts at: 0 893 1818
Size: 893 925
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 23 1609 1818
Size: 23 1586 209
Fragments arranged by size:
1586 209 23
BseYI C'CCAG_C
Cuts at: 0 23 1544 1818
Size: 23 1521 274
Fragments arranged by size:
1521 274 23
BsmI GAATG_Cn'
Cuts at: 0 962 1818
Size: 962 856
Bsp1286I G_dGCh'C
Cuts at: 0 15 783 1818
Size: 15 768 1035
Fragments arranged by size:
1035 768 15
BspEI T'CCGG_A
Cuts at: 0 893 1818
Size: 893 925
BsrDI GCAATG_nn'
Cuts at: 0 784 1818
Size: 784 1034
BstBI TT'CG_AA
Cuts at: 0 1521 1782 1818
Size: 1521 261 36
Fragments arranged by size:
1521 261 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1594 1818
Size: 1594 224
BstYI r'GATC_y
Cuts at: 0 597 711 1257 1818
Size: 597 114 546 561
Fragments arranged by size:
597 561 546 114
BtgI C'CryG_G
Cuts at: 0 31 1818
Size: 31 1787
BtsI GCAGTG_nn'
Cuts at: 0 262 1239 1818
Size: 262 977 579
Fragments arranged by size:
977 579 262
EaeI y'GGCC_r
Cuts at: 0 1103 1818
Size: 1103 715
EarI CTCTTCn'nnn_
Cuts at: 0 1426 1818
Size: 1426 392
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 724 1818
Size: 724 1094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 161 674 1011 1570 1726 1818
Size: 152 9 513 337 559 156 92
Fragments arranged by size:
559 513 337 156 152 92 9
EcoO109I rG'GnC_Cy
Cuts at: 0 1144 1818
Size: 1144 674
EcoRV GAT'ATC
Cuts at: 0 150 1818
Size: 150 1668
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1534 1818
Size: 1534 284
FspI TGC'GCA
Cuts at: 0 1486 1818
Size: 1486 332
HaeII r_GCGC'y
Cuts at: 0 360 1818
Size: 360 1458
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1190 1222 1818
Size: 1190 32 596
Fragments arranged by size:
1190 596 32
HincII GTy'rAC
Cuts at: 0 885 1818
Size: 885 933
HindIII A'AGCT_T
Cuts at: 0 1388 1818
Size: 1388 430
MslI CAynn'nnrTG
Cuts at: 0 805 1294 1654 1818
Size: 805 489 360 164
Fragments arranged by size:
805 489 360 164
MspA1I CmG'CkG
Cuts at: 0 33 970 1818
Size: 33 937 848
Fragments arranged by size:
937 848 33
NspI r_CATG'y
Cuts at: 0 891 1485 1818
Size: 891 594 333
Fragments arranged by size:
891 594 333
PciI A'CATG_T
Cuts at: 0 887 1818
Size: 887 931
PflMI CCAn_nnn'nTGG
Cuts at: 0 244 302 1818
Size: 244 58 1516
Fragments arranged by size:
1516 244 58
PpuMI rG'GwC_Cy
Cuts at: 0 1144 1818
Size: 1144 674
PshAI GACnn'nnGTC
Cuts at: 0 816 1818
Size: 816 1002
PvuII CAG'CTG
Cuts at: 0 970 1818
Size: 970 848
SacII CC_GC'GG
Cuts at: 0 34 1818
Size: 34 1784
SfcI C'TryA_G
Cuts at: 0 1373 1818
Size: 1373 445
SmaI CCC'GGG
Cuts at: 0 1254 1818
Size: 1254 564
SmlI C'TyrA_G
Cuts at: 0 491 1818
Size: 491 1327
SpeI A'CTAG_T
Cuts at: 0 1353 1818
Size: 1353 465
SphI G_CATG'C
Cuts at: 0 1485 1818
Size: 1485 333
StuI AGG'CCT
Cuts at: 0 1493 1818
Size: 1493 325
StyI C'CwwG_G
Cuts at: 0 1711 1818
Size: 1711 107
TatI w'GTAC_w
Cuts at: 0 1164 1818
Size: 1164 654
XmaI C'CCGG_G
Cuts at: 0 1252 1818
Size: 1252 566
XmnI GAAnn'nnTTC
Cuts at: 0 1517 1607 1818
Size: 1517 90 211
Fragments arranged by size:
1517 211 90
Enzymes that do cut:
AcuI AfeI AflIII AlwNI ApoI AseI AvaI BamHI
BanII BbsI BcgI BplI BpmI BpuEI BsaBI BsaWI
BseRI BseYI BsmI Bsp1286I BspEI BsrDI BstBI BstXI
BstYI BtgI BtsI EaeI EarI EciI Eco57MI EcoO109I
EcoRV FalI FspI HaeII Hin4I HincII HindIII MslI
MspA1I NspI PciI PflMI PpuMI PshAI PvuII SacII
SfcI SmaI SmlI SpeI SphI StuI StyI TatI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BanI BbeI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI Bpu10I BsaI
BsaAI BsaHI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmBI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoNI EcoRI FseI FspAI HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI ZraI