Restriction map for
pFN21AE1167
(Linear) (Six-base) MAPSORT of: KIEE1167.seq Check: 6685 from: 1 to: 1794
KIEE1167.orf
With 182 enzymes: SgfI *
January 29, 2014 11:44 ..
AatII G_ACGT'C
Cuts at: 0 1233 1794
Size: 1233 561
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 109 1741 1794
Size: 107 2 1632 53
Fragments arranged by size:
1632 107 53 2
AflIII A'CryG_T
Cuts at: 0 101 1794
Size: 101 1693
ApaI G_GGCC'C
Cuts at: 0 70 739 1794
Size: 70 669 1055
Fragments arranged by size:
1055 669 70
ApoI r'AATT_y
Cuts at: 0 940 1794
Size: 940 854
AvaI C'yCGr_G
Cuts at: 0 1576 1794
Size: 1576 218
BanI G'GyrC_C
Cuts at: 0 693 742 798 1794
Size: 693 49 56 996
Fragments arranged by size:
996 693 56 49
BanII G_rGCy'C
Cuts at: 0 70 649 739 1738 1794
Size: 70 579 90 999 56
Fragments arranged by size:
999 579 90 70 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 232 1185 1794
Size: 232 953 609
Fragments arranged by size:
953 609 232
BciVI GTATCCnnnnn_n'
Cuts at: 0 770 1794
Size: 770 1024
BfrBI ATG'CAT
Cuts at: 0 874 1794
Size: 874 920
BglI GCCn_nnn'nGGC
Cuts at: 0 751 1794
Size: 751 1043
BlpI GC'TnA_GC
Cuts at: 0 834 1174 1794
Size: 834 340 620
Fragments arranged by size:
834 620 340
Bme1580I G_kGCm'C
Cuts at: 0 70 165 739 803 1794
Size: 70 95 574 64 991
Fragments arranged by size:
991 574 95 70 64
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1110 1638 1794
Size: 1110 528 156
Fragments arranged by size:
1110 528 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 528 1708 1794
Size: 396 132 1180 86
Fragments arranged by size:
1180 396 132 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1663 1794
Size: 1663 131
BsaI GGTCTCn'nnnn_
Cuts at: 0 953 1563 1794
Size: 953 610 231
Fragments arranged by size:
953 610 231
BsaHI Gr'CG_yC
Cuts at: 0 333 1230 1794
Size: 333 897 564
Fragments arranged by size:
897 564 333
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1499 1794
Size: 1499 295
BseYI C'CCAG_C
Cuts at: 0 130 1341 1794
Size: 130 1211 453
Fragments arranged by size:
1211 453 130
BsiEI CG_ry'CG
Cuts at: 0 722 1145 1794
Size: 722 423 649
Fragments arranged by size:
722 649 423
BsiHKAI G_wGCw'C
Cuts at: 0 361 1134 1141 1794
Size: 361 773 7 653
Fragments arranged by size:
773 653 361 7
Bsp1286I G_dGCh'C
Cuts at: 0 70 165 361 649 739 803 1134 1141
Size: 70 95 196 288 90 64 331 7
Cuts at: 1141 1738 1794
Size: 597 56
Fragments arranged by size:
597 331 288 196 95 90 70 64
56 7
BsrFI r'CCGG_y
Cuts at: 0 717 1794
Size: 717 1077
BssSI C'ACGA_G
Cuts at: 0 295 457 589 1623 1660 1794
Size: 295 162 132 1034 37 134
Fragments arranged by size:
1034 295 162 134 132 37
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1479 1794
Size: 1479 315
BstYI r'GATC_y
Cuts at: 0 415 584 1794
Size: 415 169 1210
Fragments arranged by size:
1210 415 169
Bsu36I CC'TnA_GG
Cuts at: 0 449 794 1794
Size: 449 345 1000
Fragments arranged by size:
1000 449 345
BtgI C'CryG_G
Cuts at: 0 821 1794
Size: 821 973
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1394 1794
Size: 1394 400
BtsI GCAGTG_nn'
Cuts at: 0 153 629 1794
Size: 153 476 1165
Fragments arranged by size:
1165 476 153
ClaI AT'CG_AT
Cuts at: 0 1784 1794
Size: 1784 10
DraIII CAC_nnn'GTG
Cuts at: 0 297 1794
Size: 297 1497
EaeI y'GGCC_r
Cuts at: 0 824 1142 1794
Size: 824 318 652
Fragments arranged by size:
824 652 318
EagI C'GGCC_G
Cuts at: 0 1142 1794
Size: 1142 652
EarI CTCTTCn'nnn_
Cuts at: 0 85 951 1794
Size: 85 866 843
Fragments arranged by size:
866 843 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 109 396 528 1708 1741 1794
Size: 107 2 287 132 1180 33 53
Fragments arranged by size:
1180 287 132 107 53 33 2
EcoO109I rG'GnC_Cy
Cuts at: 0 66 380 736 1551 1794
Size: 66 314 356 815 243
Fragments arranged by size:
815 356 314 243 66
HaeII r_GCGC'y
Cuts at: 0 1181 1794
Size: 1181 613
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 1572 1604 1794
Size: 463 32 1077 32 190
Fragments arranged by size:
1077 463 190 32 32
HincII GTy'rAC
Cuts at: 0 201 843 1794
Size: 201 642 951
Fragments arranged by size:
951 642 201
HpaI GTT'AAC
Cuts at: 0 843 1794
Size: 843 951
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 1794
Size: 702 1092
MscI TGG'CCA
Cuts at: 0 826 1794
Size: 826 968
MslI CAynn'nnrTG
Cuts at: 0 139 1264 1678 1794
Size: 139 1125 414 116
Fragments arranged by size:
1125 414 139 116
MspA1I CmG'CkG
Cuts at: 0 495 709 1147 1794
Size: 495 214 438 647
Fragments arranged by size:
647 495 438 214
NcoI C'CATG_G
Cuts at: 0 821 1794
Size: 821 973
NsiI A_TGCA'T
Cuts at: 0 876 1794
Size: 876 918
PasI CC'CwG_GG
Cuts at: 0 1743 1794
Size: 1743 51
PflMI CCAn_nnn'nTGG
Cuts at: 0 1348 1749 1794
Size: 1348 401 45
Fragments arranged by size:
1348 401 45
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 278 310 1794
Size: 278 32 1484
Fragments arranged by size:
1484 278 32
PpuMI rG'GwC_Cy
Cuts at: 0 380 1794
Size: 380 1414
PspOMI G'GGCC_C
Cuts at: 0 66 735 1794
Size: 66 669 1059
Fragments arranged by size:
1059 669 66
PstI C_TGCA'G
Cuts at: 0 819 1794
Size: 819 975
PvuII CAG'CTG
Cuts at: 0 495 1794
Size: 495 1299
ScaI AGT'ACT
Cuts at: 0 1768 1794
Size: 1768 26
SfcI C'TryA_G
Cuts at: 0 815 1014 1794
Size: 815 199 780
Fragments arranged by size:
815 780 199
SmaI CCC'GGG
Cuts at: 0 1578 1794
Size: 1578 216
SmlI C'TyrA_G
Cuts at: 0 1642 1794
Size: 1642 152
SpeI A'CTAG_T
Cuts at: 0 786 1794
Size: 786 1008
StyI C'CwwG_G
Cuts at: 0 821 1794
Size: 821 973
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 797 1072 1794
Size: 797 275 722
Fragments arranged by size:
797 722 275
TatI w'GTAC_w
Cuts at: 0 1766 1794
Size: 1766 28
Tth111I GACn'n_nGTC
Cuts at: 0 664 1439 1794
Size: 664 775 355
Fragments arranged by size:
775 664 355
XmaI C'CCGG_G
Cuts at: 0 1576 1794
Size: 1576 218
XmnI GAAnn'nnTTC
Cuts at: 0 1297 1794
Size: 1297 497
ZraI GAC'GTC
Cuts at: 0 1231 1794
Size: 1231 563
Enzymes that do cut:
AatII AcuI AflIII ApaI ApoI AvaI BanI BanII
BbsI BciVI BfrBI BglI BlpI Bme1580I BplI BpmI
BpuEI BsaI BsaHI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BsrFI BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraIII EaeI EagI EarI Eco57MI EcoO109I HaeII
Hin4I HincII HpaI MmeI MscI MslI MspA1I NcoI
NsiI PasI PflMI PpiI PpuMI PspOMI PstI PvuII
ScaI SfcI SmaI SmlI SpeI StyI TaqII TatI
Tth111I XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BclI BglII
BmgBI BmrI BmtI Bpu10I BsaAI BsaBI BsaWI BsaXI
BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I CspCI
DraI DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HindIII KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NspI
PacI PciI PfoI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SphI
SrfI SspI StuI SwaI TstI XbaI XcmI XhoI