Restriction map for
pFN21AE1165
(Linear) (Six-base) MAPSORT of: KIEE1165.seq Check: 5549 from: 1 to: 1782
KIEE1165.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 603 1193 1739 1782
Size: 574 29 590 546 43
Fragments arranged by size:
590 574 546 43 29
AfeI AGC'GCT
Cuts at: 0 1209 1782
Size: 1209 573
AlwNI CAG_nnn'CTG
Cuts at: 0 55 1782
Size: 55 1727
ApaI G_GGCC'C
Cuts at: 0 422 1782
Size: 422 1360
ApoI r'AATT_y
Cuts at: 0 405 770 1243 1782
Size: 405 365 473 539
Fragments arranged by size:
539 473 405 365
AvaI C'yCGr_G
Cuts at: 0 1548 1576 1782
Size: 1548 28 206
Fragments arranged by size:
1548 206 28
BanI G'GyrC_C
Cuts at: 0 117 1782
Size: 117 1665
BanII G_rGCy'C
Cuts at: 0 422 1782
Size: 422 1360
BbeI G_GCGC'C
Cuts at: 0 121 1782
Size: 121 1661
BbsI GAAGACnn'nnnn_
Cuts at: 0 609 1742 1782
Size: 609 1133 40
Fragments arranged by size:
1133 609 40
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 889 923 1782
Size: 889 34 859
Fragments arranged by size:
889 859 34
BclI T'GATC_A
Cuts at: 0 848 1782
Size: 848 934
BglI GCCn_nnn'nGGC
Cuts at: 0 1057 1782
Size: 1057 725
BlpI GC'TnA_GC
Cuts at: 0 1136 1782
Size: 1136 646
Bme1580I G_kGCm'C
Cuts at: 0 422 1782
Size: 422 1360
BmrI ACTGGGnnnn_n'
Cuts at: 0 533 1782
Size: 533 1249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 935 1610 1782
Size: 935 675 172
Fragments arranged by size:
935 675 172
Bpu10I CC'TnA_GC
Cuts at: 0 1404 1782
Size: 1404 378
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 1587 1782
Size: 416 1171 195
Fragments arranged by size:
1171 416 195
BsaHI Gr'CG_yC
Cuts at: 0 118 1739 1782
Size: 118 1621 43
Fragments arranged by size:
1621 118 43
BsaWI w'CCGG_w
Cuts at: 0 158 1782
Size: 158 1624
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 545 1594 1597 1782
Size: 545 1049 3 185
Fragments arranged by size:
1049 545 185 3
BseYI C'CCAG_C
Cuts at: 0 1197 1256 1782
Size: 1197 59 526
Fragments arranged by size:
1197 526 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 1463 1615 1782
Size: 172 1291 152 167
Fragments arranged by size:
1291 172 167 152
Bsp1286I G_dGCh'C
Cuts at: 0 422 1782
Size: 422 1360
BspEI T'CCGG_A
Cuts at: 0 158 1782
Size: 158 1624
BsrDI GCAATG_nn'
Cuts at: 0 1105 1782
Size: 1105 677
BsrFI r'CCGG_y
Cuts at: 0 1078 1782
Size: 1078 704
BssSI C'ACGA_G
Cuts at: 0 1073 1782
Size: 1073 709
BstYI r'GATC_y
Cuts at: 0 155 1747 1782
Size: 155 1592 35
Fragments arranged by size:
1592 155 35
BtgI C'CryG_G
Cuts at: 0 452 1782
Size: 452 1330
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 447 1782
Size: 447 1335
BtsI GCAGTG_nn'
Cuts at: 0 1763 1782
Size: 1763 19
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 359 394 1782
Size: 359 35 1388
Fragments arranged by size:
1388 359 35
DraI TTT'AAA
Cuts at: 0 1313 1782
Size: 1313 469
DrdI GACnn_nn'nnGTC
Cuts at: 0 1267 1782
Size: 1267 515
EaeI y'GGCC_r
Cuts at: 0 1388 1782
Size: 1388 394
EarI CTCTTCn'nnn_
Cuts at: 0 4 137 302 750 1502 1782
Size: 4 133 165 448 752 280
Fragments arranged by size:
752 448 280 165 133 4
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 603 935 1193 1610 1739 1782
Size: 574 29 332 258 417 129 43
Fragments arranged by size:
574 417 332 258 129 43 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 125 1579 1782
Size: 125 1454 203
Fragments arranged by size:
1454 203 125
EcoO109I rG'GnC_Cy
Cuts at: 0 418 419 1782
Size: 418 1 1363
Fragments arranged by size:
1363 418 1
EcoRV GAT'ATC
Cuts at: 0 339 1782
Size: 339 1443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 90 1782
Size: 90 1692
HaeII r_GCGC'y
Cuts at: 0 121 1211 1782
Size: 121 1090 571
Fragments arranged by size:
1090 571 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 147 179 666 698 789 821 979 1011
Size: 147 32 487 32 91 32 158 32
Cuts at: 1011 1285 1317 1607 1639 1782
Size: 274 32 290 32 143
Fragments arranged by size:
487 290 274 158 147 143 91 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 297 1452 1782
Size: 297 1155 330
Fragments arranged by size:
1155 330 297
HindIII A'AGCT_T
Cuts at: 0 1018 1782
Size: 1018 764
HpaI GTT'AAC
Cuts at: 0 297 1782
Size: 297 1485
KasI G'GCGC_C
Cuts at: 0 117 1782
Size: 117 1665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 1508 1782
Size: 974 534 274
Fragments arranged by size:
974 534 274
MscI TGG'CCA
Cuts at: 0 1390 1782
Size: 1390 392
MslI CAynn'nnrTG
Cuts at: 0 683 1782
Size: 683 1099
MspA1I CmG'CkG
Cuts at: 0 881 1447 1782
Size: 881 566 335
Fragments arranged by size:
881 566 335
NaeI GCC'GGC
Cuts at: 0 1080 1782
Size: 1080 702
NarI GG'CG_CC
Cuts at: 0 118 1782
Size: 118 1664
NcoI C'CATG_G
Cuts at: 0 452 1782
Size: 452 1330
NgoMIV G'CCGG_C
Cuts at: 0 1078 1782
Size: 1078 704
PflMI CCAn_nnn'nTGG
Cuts at: 0 1476 1782
Size: 1476 306
PspOMI G'GGCC_C
Cuts at: 0 418 1782
Size: 418 1364
PspXI vC'TCGA_Gb
Cuts at: 0 1548 1576 1782
Size: 1548 28 206
Fragments arranged by size:
1548 206 28
PstI C_TGCA'G
Cuts at: 0 101 902 1782
Size: 101 801 880
Fragments arranged by size:
880 801 101
PvuII CAG'CTG
Cuts at: 0 1447 1782
Size: 1447 335
SexAI A'CCwGG_T
Cuts at: 0 1687 1782
Size: 1687 95
SfcI C'TryA_G
Cuts at: 0 47 97 729 898 1782
Size: 47 50 632 169 884
Fragments arranged by size:
884 632 169 50 47
SfoI GGC'GCC
Cuts at: 0 119 1782
Size: 119 1663
SmlI C'TyrA_G
Cuts at: 0 431 1548 1566 1576 1782
Size: 431 1117 18 10 206
Fragments arranged by size:
1117 431 206 18 10
StyI C'CwwG_G
Cuts at: 0 452 1229 1518 1782
Size: 452 777 289 264
Fragments arranged by size:
777 452 289 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 452 1782
Size: 452 1330
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 718 750 1782
Size: 718 32 1032
Fragments arranged by size:
1032 718 32
Tth111I GACn'n_nGTC
Cuts at: 0 1689 1782
Size: 1689 93
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 134 1782
Size: 134 1648
XhoI C'TCGA_G
Cuts at: 0 1548 1576 1782
Size: 1548 28 206
Fragments arranged by size:
1548 206 28
Enzymes that do cut:
AcuI AfeI AlwNI ApaI ApoI AvaI BanI BanII
BbeI BbsI BcgI BclI BglI BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaHI BsaWI BseRI BseYI BsgI
Bsp1286I BspEI BsrDI BsrFI BssSI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII HpaI
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV PflMI PspOMI PspXI PstI PvuII SexAI SfcI
SfoI SmlI StyI TaqII TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BciVI BfrBI BglII BmgBI
BmtI BplI BsaI BsaAI BsaBI BsaXI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EagI EciI EcoICRI EcoRI FseI FspI FspAI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
XbaI XmaI XmnI ZraI