Restriction map for
pFN21AE1164
(Linear) (Six-base) MAPSORT of: KIEE1164.seq Check: 6245 from: 1 to: 1779
KIEE1164.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AccI GT'mk_AC
Cuts at: 0 783 1779
Size: 783 996
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1115 1779
Size: 1115 664
AflII C'TTAA_G
Cuts at: 0 496 1779
Size: 496 1283
AflIII A'CryG_T
Cuts at: 0 1447 1779
Size: 1447 332
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 831 1779
Size: 831 948
ApaLI G'TGCA_C
Cuts at: 0 491 1779
Size: 491 1288
ApoI r'AATT_y
Cuts at: 0 133 1040 1111 1779
Size: 133 907 71 668
Fragments arranged by size:
907 668 133 71
AseI AT'TA_AT
Cuts at: 0 887 1779
Size: 887 892
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 1498 1779
Size: 248 1250 281
Fragments arranged by size:
1250 281 248
BglII A'GATC_T
Cuts at: 0 1459 1779
Size: 1459 320
BlpI GC'TnA_GC
Cuts at: 0 1253 1779
Size: 1253 526
Bme1580I G_kGCm'C
Cuts at: 0 495 1779
Size: 495 1284
BmgBI CAC'GTC
Cuts at: 0 1717 1779
Size: 1717 62
BmrI ACTGGGnnnn_n'
Cuts at: 0 906 1779
Size: 906 873
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 935 982 1419 1507 1779
Size: 647 288 47 437 88 272
Fragments arranged by size:
647 437 288 272 88 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1779
Size: 889 890
BsaI GGTCTCn'nnnn_
Cuts at: 0 50 1779
Size: 50 1729
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1319 1762 1779
Size: 1319 443 17
Fragments arranged by size:
1319 443 17
BsiHKAI G_wGCw'C
Cuts at: 0 495 1521 1779
Size: 495 1026 258
Fragments arranged by size:
1026 495 258
Bsp1286I G_dGCh'C
Cuts at: 0 495 1521 1779
Size: 495 1026 258
Fragments arranged by size:
1026 495 258
BspHI T'CATG_A
Cuts at: 0 530 678 1107 1779
Size: 530 148 429 672
Fragments arranged by size:
672 530 429 148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 10 568 1666 1779
Size: 10 558 1098 113
Fragments arranged by size:
1098 558 113 10
BsrDI GCAATG_nn'
Cuts at: 0 520 700 1176 1779
Size: 520 180 476 603
Fragments arranged by size:
603 520 476 180
BsrGI T'GTAC_A
Cuts at: 0 313 1779
Size: 313 1466
BstXI CCAn_nnnn'nTGG
Cuts at: 0 354 794 1779
Size: 354 440 985
Fragments arranged by size:
985 440 354
BstYI r'GATC_y
Cuts at: 0 1459 1779
Size: 1459 320
BtsI GCAGTG_nn'
Cuts at: 0 463 607 676 1779
Size: 463 144 69 1103
Fragments arranged by size:
1103 463 144 69
DraI TTT'AAA
Cuts at: 0 1116 1779
Size: 1116 663
DrdI GACnn_nn'nnGTC
Cuts at: 0 90 1779
Size: 90 1689
EaeI y'GGCC_r
Cuts at: 0 375 1779
Size: 375 1404
EarI CTCTTCn'nnn_
Cuts at: 0 134 323 1491 1509 1779
Size: 134 189 1168 18 270
Fragments arranged by size:
1168 270 189 134 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 935 982 1115 1419 1507 1779
Size: 647 288 47 133 304 88 272
Fragments arranged by size:
647 304 288 272 133 88 47
EcoO109I rG'GnC_Cy
Cuts at: 0 622 1779
Size: 622 1157
EcoRI G'AATT_C
Cuts at: 0 1040 1779
Size: 1040 739
EcoRV GAT'ATC
Cuts at: 0 385 1779
Size: 385 1394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 870 1779
Size: 870 909
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1258 1290 1779
Size: 1258 32 489
Fragments arranged by size:
1258 489 32
MluI A'CGCG_T
Cuts at: 0 1447 1779
Size: 1447 332
MscI TGG'CCA
Cuts at: 0 377 1779
Size: 377 1402
MslI CAynn'nnrTG
Cuts at: 0 792 813 991 1779
Size: 792 21 178 788
Fragments arranged by size:
792 788 178 21
NspI r_CATG'y
Cuts at: 0 131 958 1691 1779
Size: 131 827 733 88
Fragments arranged by size:
827 733 131 88
PstI C_TGCA'G
Cuts at: 0 757 1677 1779
Size: 757 920 102
Fragments arranged by size:
920 757 102
SapI GCTCTTCn'nnn_
Cuts at: 0 1509 1779
Size: 1509 270
SfcI C'TryA_G
Cuts at: 0 11 549 649 753 1202 1313 1673 1779
Size: 11 538 100 104 449 111 360 106
Fragments arranged by size:
538 449 360 111 106 104 100 11
SmlI C'TyrA_G
Cuts at: 0 496 868 1779
Size: 496 372 911
Fragments arranged by size:
911 496 372
StyI C'CwwG_G
Cuts at: 0 378 1779
Size: 378 1401
TatI w'GTAC_w
Cuts at: 0 313 1779
Size: 313 1466
Enzymes that do cut:
AccI AcuI AflII AflIII AlfI ApaLI ApoI AseI
BbsI BglII BlpI Bme1580I BmgBI BmrI BpmI BpuEI
BsaI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrGI
BstXI BstYI BtsI DraI DrdI EaeI EarI Eco57MI
EcoO109I EcoRI EcoRV FalI Hin4I MluI MscI MslI
NspI PstI SapI SfcI SmlI StyI TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AloI AlwNI ApaI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BmtI BplI Bpu10I BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII EagI
EciI EcoICRI EcoNI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MmeI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI