Restriction map for
pFN21AE1163
(Linear) (Six-base) MAPSORT of: KIEE1163.seq Check: 3488 from: 1 to: 1740
KIEE1163.orf
With 182 enzymes: SgfI *
July 3, 2012 16:48 ..
AatII G_ACGT'C
Cuts at: 0 1148 1740
Size: 1148 592
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 584 879 1740
Size: 182 402 295 861
Fragments arranged by size:
861 402 295 182
AflIII A'CryG_T
Cuts at: 0 8 955 1740
Size: 8 947 785
Fragments arranged by size:
947 785 8
AleI CACnn'nnGTG
Cuts at: 0 1113 1740
Size: 1113 627
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 383 1740
Size: 383 1357
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 679 711 1740
Size: 679 32 1029
Fragments arranged by size:
1029 679 32
AlwNI CAG_nnn'CTG
Cuts at: 0 208 226 514 562 1491 1740
Size: 208 18 288 48 929 249
Fragments arranged by size:
929 288 249 208 48 18
ApaI G_GGCC'C
Cuts at: 0 496 810 1740
Size: 496 314 930
Fragments arranged by size:
930 496 314
ApoI r'AATT_y
Cuts at: 0 787 820 1734 1740
Size: 787 33 914 6
Fragments arranged by size:
914 787 33 6
AvaI C'yCGr_G
Cuts at: 0 87 797 1740
Size: 87 710 943
Fragments arranged by size:
943 710 87
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1134 1167 1740
Size: 1134 33 573
Fragments arranged by size:
1134 573 33
BamHI G'GATC_C
Cuts at: 0 1658 1740
Size: 1658 82
BanII G_rGCy'C
Cuts at: 0 19 496 810 1740
Size: 19 477 314 930
Fragments arranged by size:
930 477 314 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 1381 1740
Size: 1381 359
BbvCI CC'TCA_GC
Cuts at: 0 1514 1740
Size: 1514 226
BciVI GTATCCnnnnn_n'
Cuts at: 0 963 1740
Size: 963 777
Bme1580I G_kGCm'C
Cuts at: 0 95 496 810 1740
Size: 95 401 314 930
Fragments arranged by size:
930 401 314 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 145 1740
Size: 145 1595
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1740
Size: 688 1052
Bpu10I CC'TnA_GC
Cuts at: 0 182 374 1514 1740
Size: 182 192 1140 226
Fragments arranged by size:
1140 226 192 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 1740
Size: 320 1420
BsaI GGTCTCn'nnnn_
Cuts at: 0 406 1740
Size: 406 1334
BsaAI yAC'GTr
Cuts at: 0 11 1740
Size: 11 1729
BsaBI GATnn'nnATC
Cuts at: 0 419 662 869 1740
Size: 419 243 207 871
Fragments arranged by size:
871 419 243 207
BsaHI Gr'CG_yC
Cuts at: 0 1145 1740
Size: 1145 595
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1311 1341 1740
Size: 1311 30 399
Fragments arranged by size:
1311 399 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 859 1740
Size: 859 881
BseYI C'CCAG_C
Cuts at: 0 187 1740
Size: 187 1553
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 557 874 1112 1740
Size: 146 411 317 238 628
Fragments arranged by size:
628 411 317 238 146
BsiHKAI G_wGCw'C
Cuts at: 0 19 1740
Size: 19 1721
Bsp1286I G_dGCh'C
Cuts at: 0 19 95 496 810 1740
Size: 19 76 401 314 930
Fragments arranged by size:
930 401 314 76 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 982 1740
Size: 982 758
BsrDI GCAATG_nn'
Cuts at: 0 274 1740
Size: 274 1466
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1115 1314 1654 1740
Size: 1115 199 340 86
Fragments arranged by size:
1115 340 199 86
BstYI r'GATC_y
Cuts at: 0 99 222 734 1118 1658 1740
Size: 99 123 512 384 540 82
Fragments arranged by size:
540 512 384 123 99 82
BtgI C'CryG_G
Cuts at: 0 1307 1431 1740
Size: 1307 124 309
Fragments arranged by size:
1307 309 124
BtsI GCAGTG_nn'
Cuts at: 0 1443 1740
Size: 1443 297
DraIII CAC_nnn'GTG
Cuts at: 0 1072 1617 1740
Size: 1072 545 123
Fragments arranged by size:
1072 545 123
EarI CTCTTCn'nnn_
Cuts at: 0 603 1108 1288 1740
Size: 603 505 180 452
Fragments arranged by size:
603 505 452 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 90 1740
Size: 90 1650
EcoICRI GAG'CTC
Cuts at: 0 17 1740
Size: 17 1723
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 584 688 879 1740
Size: 182 402 104 191 861
Fragments arranged by size:
861 402 191 182 104
EcoO109I rG'GnC_Cy
Cuts at: 0 492 493 1020 1220 1740
Size: 492 1 527 200 520
Fragments arranged by size:
527 520 492 200 1
EcoRI G'AATT_C
Cuts at: 0 820 1740
Size: 820 920
EcoRV GAT'ATC
Cuts at: 0 1131 1740
Size: 1131 609
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 909 1740
Size: 909 831
FspI TGC'GCA
Cuts at: 0 1243 1740
Size: 1243 497
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 277 309 890 922 1311 1343 1740
Size: 277 32 581 32 389 32 397
Fragments arranged by size:
581 397 389 277 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1056 1740
Size: 154 902 684
Fragments arranged by size:
902 684 154
MslI CAynn'nnrTG
Cuts at: 0 920 1113 1740
Size: 920 193 627
Fragments arranged by size:
920 627 193
MspA1I CmG'CkG
Cuts at: 0 42 69 1740
Size: 42 27 1671
Fragments arranged by size:
1671 42 27
NcoI C'CATG_G
Cuts at: 0 1307 1431 1740
Size: 1307 124 309
Fragments arranged by size:
1307 309 124
NspI r_CATG'y
Cuts at: 0 649 959 1740
Size: 649 310 781
Fragments arranged by size:
781 649 310
PasI CC'CwG_GG
Cuts at: 0 496 1190 1740
Size: 496 694 550
Fragments arranged by size:
694 550 496
PciI A'CATG_T
Cuts at: 0 955 1740
Size: 955 785
PflMI CCAn_nnn'nTGG
Cuts at: 0 1491 1740
Size: 1491 249
PmlI CAC'GTG
Cuts at: 0 11 1740
Size: 11 1729
PpuMI rG'GwC_Cy
Cuts at: 0 1220 1740
Size: 1220 520
PspOMI G'GGCC_C
Cuts at: 0 492 806 1740
Size: 492 314 934
Fragments arranged by size:
934 492 314
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1502 1534 1740
Size: 1502 32 206
Fragments arranged by size:
1502 206 32
PstI C_TGCA'G
Cuts at: 0 68 362 1740
Size: 68 294 1378
Fragments arranged by size:
1378 294 68
PvuII CAG'CTG
Cuts at: 0 42 1740
Size: 42 1698
SacI G_AGCT'C
Cuts at: 0 19 1740
Size: 19 1721
SanDI GG'GwC_CC
Cuts at: 0 1220 1740
Size: 1220 520
SfcI C'TryA_G
Cuts at: 0 64 218 358 554 1264 1740
Size: 64 154 140 196 710 476
Fragments arranged by size:
710 476 196 154 140 64
SmlI C'TyrA_G
Cuts at: 0 335 797 1740
Size: 335 462 943
Fragments arranged by size:
943 462 335
StyI C'CwwG_G
Cuts at: 0 764 839 1307 1431 1473 1638 1740
Size: 764 75 468 124 42 165 102
Fragments arranged by size:
764 468 165 124 102 75 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 180 460 1112 1359 1740
Size: 180 280 652 247 381
Fragments arranged by size:
652 381 280 247 180
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1110 1142 1740
Size: 1110 32 598
Fragments arranged by size:
1110 598 32
Tth111I GACn'n_nGTC
Cuts at: 0 878 1740
Size: 878 862
XhoI C'TCGA_G
Cuts at: 0 797 1740
Size: 797 943
XmnI GAAnn'nnTTC
Cuts at: 0 788 1740
Size: 788 952
ZraI GAC'GTC
Cuts at: 0 1146 1740
Size: 1146 594
Enzymes that do cut:
AatII AcuI AflIII AleI AlfI AloI AlwNI ApaI
ApoI AvaI BaeI BamHI BanII BbsI BbvCI BciVI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrDI BstXI BstYI BtgI BtsI DraIII EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI Hin4I
MmeI MslI MspA1I NcoI NspI PasI PciI PflMI
PmlI PpuMI PspOMI PsrI PstI PvuII SacI SanDI
SfcI SmlI StyI TaqII TstI Tth111I XhoI XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
ApaLI AscI AseI AsiSI AvrII BanI BbeI BcgI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EaeI EagI
EcoNI FseI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PfoI PmeI PpiI
PshAI PsiI PspXI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
XbaI XcmI XmaI