Restriction map for
pFN21AE1162
(Linear) (Six-base) MAPSORT of: KIEE1162.seq Check: 6733 from: 1 to: 1701
KIEE1162.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 521 945 1197 1449 1638 1701
Size: 276 245 424 252 252 189 63
Fragments arranged by size:
424 276 252 252 245 189 63
AflIII A'CryG_T
Cuts at: 0 1019 1021 1701
Size: 1019 2 680
Fragments arranged by size:
1019 680 2
AhdI GACnn_n'nnGTC
Cuts at: 0 734 1701
Size: 734 967
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 50 82 1701
Size: 50 32 1619
Fragments arranged by size:
1619 50 32
ApoI r'AATT_y
Cuts at: 0 509 969 1163 1247 1701
Size: 509 460 194 84 454
Fragments arranged by size:
509 460 454 194 84
AvaI C'yCGr_G
Cuts at: 0 152 1701
Size: 152 1549
BbsI GAAGACnn'nnnn_
Cuts at: 0 951 1203 1701
Size: 951 252 498
Fragments arranged by size:
951 498 252
BbvCI CC'TCA_GC
Cuts at: 0 144 1701
Size: 144 1557
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 956 990 1701
Size: 956 34 711
Fragments arranged by size:
956 711 34
BfrBI ATG'CAT
Cuts at: 0 665 1701
Size: 665 1036
BglII A'GATC_T
Cuts at: 0 171 1701
Size: 171 1530
BmrI ACTGGGnnnn_n'
Cuts at: 0 1181 1701
Size: 1181 520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 773 857 941 1277 1361 1445 1529
Size: 97 676 84 84 336 84 84 84
Cuts at: 1529 1701
Size: 172
Fragments arranged by size:
676 336 172 97 84 84 84 84
84
Bpu10I CC'TnA_GC
Cuts at: 0 144 1701
Size: 144 1557
BsaI GGTCTCn'nnnn_
Cuts at: 0 70 395 1701
Size: 70 325 1306
Fragments arranged by size:
1306 325 70
BsaAI yAC'GTr
Cuts at: 0 1022 1701
Size: 1022 679
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 50 80 1701
Size: 50 30 1621
Fragments arranged by size:
1621 50 30
BseYI C'CCAG_C
Cuts at: 0 160 1701
Size: 160 1541
BsmI GAATG_Cn'
Cuts at: 0 1389 1473 1701
Size: 1389 84 228
Fragments arranged by size:
1389 228 84
BsrDI GCAATG_nn'
Cuts at: 0 90 1116 1284 1701
Size: 90 1026 168 417
Fragments arranged by size:
1026 417 168 90
BstAPI GCAn_nnn'nTGC
Cuts at: 0 890 1701
Size: 890 811
BstBI TT'CG_AA
Cuts at: 0 1393 1701
Size: 1393 308
BstEII G'GTnAC_C
Cuts at: 0 220 1701
Size: 220 1481
BstYI r'GATC_y
Cuts at: 0 171 1701
Size: 171 1530
BtgI C'CryG_G
Cuts at: 0 546 1701
Size: 546 1155
BtsI GCAGTG_nn'
Cuts at: 0 102 1701
Size: 102 1599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1098 1133 1266 1301 1701
Size: 1098 35 133 35 400
Fragments arranged by size:
1098 400 133 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 246 1701
Size: 246 1455
EarI CTCTTCn'nnn_
Cuts at: 0 275 411 1657 1701
Size: 275 136 1246 44
Fragments arranged by size:
1246 275 136 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 276 521 773 857 941 945 1197
Size: 97 179 245 252 84 84 4 252
Cuts at: 1197 1277 1361 1445 1449 1529 1638 1701
Size: 80 84 84 4 80 109 63
Fragments arranged by size:
252 252 245 179 109 97 84 84
84 84 80 80 63 4 4
EcoRI G'AATT_C
Cuts at: 0 1163 1247 1701
Size: 1163 84 454
Fragments arranged by size:
1163 454 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 403 1701
Size: 403 1298
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 156 188 720 752 1701
Size: 156 32 532 32 949
Fragments arranged by size:
949 532 156 32 32
HincII GTy'rAC
Cuts at: 0 543 1058 1701
Size: 543 515 643
Fragments arranged by size:
643 543 515
HindIII A'AGCT_T
Cuts at: 0 459 1701
Size: 459 1242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 1701
Size: 24 1677
MslI CAynn'nnrTG
Cuts at: 0 476 852 1188 1701
Size: 476 376 336 513
Fragments arranged by size:
513 476 376 336
NcoI C'CATG_G
Cuts at: 0 546 1701
Size: 546 1155
NsiI A_TGCA'T
Cuts at: 0 667 1701
Size: 667 1034
PasI CC'CwG_GG
Cuts at: 0 70 1701
Size: 70 1631
PfoI T'CCnGG_A
Cuts at: 0 119 1701
Size: 119 1582
PmlI CAC'GTG
Cuts at: 0 1022 1701
Size: 1022 679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 50 82 1701
Size: 50 32 1619
Fragments arranged by size:
1619 50 32
PsiI TTA'TAA
Cuts at: 0 1691 1701
Size: 1691 10
PstI C_TGCA'G
Cuts at: 0 488 1701
Size: 488 1213
SexAI A'CCwGG_T
Cuts at: 0 60 1701
Size: 60 1641
SfcI C'TryA_G
Cuts at: 0 52 484 1701
Size: 52 432 1217
Fragments arranged by size:
1217 432 52
StyI C'CwwG_G
Cuts at: 0 546 1701
Size: 546 1155
Tth111I GACn'n_nGTC
Cuts at: 0 955 1701
Size: 955 746
Enzymes that do cut:
AcuI AflIII AhdI AloI ApoI AvaI BbsI BbvCI
BcgI BfrBI BglII BmrI BpmI Bpu10I BsaI BsaAI
BsaXI BseYI BsmI BsrDI BstAPI BstBI BstEII BstYI
BtgI BtsI CspCI DraIII EarI Eco57MI EcoRI FalI
Hin4I HincII HindIII MmeI MslI NcoI NsiI PasI
PfoI PmlI PpiI PsiI PstI SexAI SfcI StyI
Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BciVI BclI
BglI BlpI Bme1580I BmgBI BmtI BplI BpuEI BsaBI
BsaHI BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstXI BstZ17I Bsu36I BtgZI ClaI DraI DrdI
EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NspI PacI PciI PflMI PmeI PpuMI PshAI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI XbaI XcmI XhoI
XmaI XmnI ZraI