Restriction map for
pFN21AE1160
(Linear) (Six-base) MAPSORT of: KIEE1160.seq Check: 5016 from: 1 to: 1581
KIEE1160.orf
With 182 enzymes: SgfI *
July 3, 2012 16:48 ..
AflIII A'CryG_T
Cuts at: 0 92 905 1127 1385 1581
Size: 92 813 222 258 196
Fragments arranged by size:
813 258 222 196 92
AhdI GACnn_n'nnGTC
Cuts at: 0 1020 1581
Size: 1020 561
AleI CACnn'nnGTG
Cuts at: 0 1126 1581
Size: 1126 455
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 493 1581
Size: 493 1088
AlwNI CAG_nnn'CTG
Cuts at: 0 12 72 81 251 294 396 1581
Size: 12 60 9 170 43 102 1185
Fragments arranged by size:
1185 170 102 60 43 12 9
ApaI G_GGCC'C
Cuts at: 0 767 1581
Size: 767 814
ApaLI G'TGCA_C
Cuts at: 0 1389 1581
Size: 1389 192
AvaI C'yCGr_G
Cuts at: 0 375 982 1581
Size: 375 607 599
Fragments arranged by size:
607 599 375
BanI G'GyrC_C
Cuts at: 0 346 793 1120 1249 1581
Size: 346 447 327 129 332
Fragments arranged by size:
447 346 332 327 129
BanII G_rGCy'C
Cuts at: 0 373 428 436 767 1581
Size: 373 55 8 331 814
Fragments arranged by size:
814 373 331 55 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 505 1581
Size: 505 1076
BbvCI CC'TCA_GC
Cuts at: 0 244 1451 1581
Size: 244 1207 130
Fragments arranged by size:
1207 244 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1351 1385 1581
Size: 1351 34 196
Fragments arranged by size:
1351 196 34
BglI GCCn_nnn'nGGC
Cuts at: 0 124 133 1378 1547 1581
Size: 124 9 1245 169 34
Fragments arranged by size:
1245 169 124 34 9
BglII A'GATC_T
Cuts at: 0 896 1581
Size: 896 685
BlpI GC'TnA_GC
Cuts at: 0 268 1581
Size: 268 1313
Bme1580I G_kGCm'C
Cuts at: 0 349 767 1393 1581
Size: 349 418 626 188
Fragments arranged by size:
626 418 349 188
BmgBI CAC'GTC
Cuts at: 0 93 773 1164 1581
Size: 93 680 391 417
Fragments arranged by size:
680 417 391 93
BmrI ACTGGGnnnn_n'
Cuts at: 0 219 1581
Size: 219 1362
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 537 1581
Size: 537 1044
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 1126 1581
Size: 70 1056 455
Fragments arranged by size:
1056 455 70
Bpu10I CC'TnA_GC
Cuts at: 0 244 1451 1581
Size: 244 1207 130
Fragments arranged by size:
1207 244 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 783 1429 1581
Size: 346 437 646 152
Fragments arranged by size:
646 437 346 152
BsaI GGTCTCn'nnnn_
Cuts at: 0 1525 1581
Size: 1525 56
BsaBI GATnn'nnATC
Cuts at: 0 22 1581
Size: 22 1559
BsaHI Gr'CG_yC
Cuts at: 0 1021 1460 1581
Size: 1021 439 121
Fragments arranged by size:
1021 439 121
BsaWI w'CCGG_w
Cuts at: 0 675 1223 1581
Size: 675 548 358
Fragments arranged by size:
675 548 358
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 421 856 865 868 883 1581
Size: 421 435 9 3 15 698
Fragments arranged by size:
698 435 421 15 9 3
BseYI C'CCAG_C
Cuts at: 0 577 692 766 1581
Size: 577 115 74 815
Fragments arranged by size:
815 577 115 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 547 1581
Size: 238 309 1034
Fragments arranged by size:
1034 309 238
BsiEI CG_ry'CG
Cuts at: 0 1382 1581
Size: 1382 199
BsiHKAI G_wGCw'C
Cuts at: 0 373 1310 1393 1445 1581
Size: 373 937 83 52 136
Fragments arranged by size:
937 373 136 83 52
BsmI GAATG_Cn'
Cuts at: 0 1374 1581
Size: 1374 207
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1028 1581
Size: 1028 553
Bsp1286I G_dGCh'C
Cuts at: 0 349 373 428 436 767 1310 1393 1445
Size: 349 24 55 8 331 543 83 52
Cuts at: 1445 1581
Size: 136
Fragments arranged by size:
543 349 331 136 83 55 52 24
8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 963 1581
Size: 963 618
BsrBI CCG'CTC
Cuts at: 0 780 1262 1581
Size: 780 482 319
Fragments arranged by size:
780 482 319
BsrDI GCAATG_nn'
Cuts at: 0 597 1581
Size: 597 984
BsrFI r'CCGG_y
Cuts at: 0 207 263 641 727 890 1252 1581
Size: 207 56 378 86 163 362 329
Fragments arranged by size:
378 362 329 207 163 86 56
BsrGI T'GTAC_A
Cuts at: 0 302 908 917 1130 1581
Size: 302 606 9 213 451
Fragments arranged by size:
606 451 302 213 9
BssSI C'ACGA_G
Cuts at: 0 484 1543 1581
Size: 484 1059 38
Fragments arranged by size:
1059 484 38
BstAPI GCAn_nnn'nTGC
Cuts at: 0 534 1581
Size: 534 1047
BstYI r'GATC_y
Cuts at: 0 23 239 323 896 1581
Size: 23 216 84 573 685
Fragments arranged by size:
685 573 216 84 23
BtgI C'CryG_G
Cuts at: 0 194 437 539 701 1581
Size: 194 243 102 162 880
Fragments arranged by size:
880 243 194 162 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1359 1581
Size: 1359 222
BtsI GCAGTG_nn'
Cuts at: 0 10 535 1581
Size: 10 525 1046
Fragments arranged by size:
1046 525 10
EaeI y'GGCC_r
Cuts at: 0 457 542 564 608 936 1235 1379 1581
Size: 457 85 22 44 328 299 144 202
Fragments arranged by size:
457 328 299 202 144 85 44 22
EagI C'GGCC_G
Cuts at: 0 1379 1581
Size: 1379 202
EarI CTCTTCn'nnn_
Cuts at: 0 1269 1581
Size: 1269 312
EcoICRI GAG'CTC
Cuts at: 0 371 1581
Size: 371 1210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 1126 1581
Size: 70 1056 455
Fragments arranged by size:
1056 455 70
EcoO109I rG'GnC_Cy
Cuts at: 0 78 168 393 411 960 1188 1558 1581
Size: 78 90 225 18 549 228 370 23
Fragments arranged by size:
549 370 228 225 90 78 23 18
FspI TGC'GCA
Cuts at: 0 1414 1581
Size: 1414 167
HaeII r_GCGC'y
Cuts at: 0 194 806 1581
Size: 194 612 775
Fragments arranged by size:
775 612 194
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 400 432 1006 1038 1581
Size: 400 32 574 32 543
Fragments arranged by size:
574 543 400 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 497 755 1264 1581
Size: 401 96 258 509 317
Fragments arranged by size:
509 401 317 258 96
MscI TGG'CCA
Cuts at: 0 459 544 566 610 1237 1581
Size: 459 85 22 44 627 344
Fragments arranged by size:
627 459 344 85 44 22
MslI CAynn'nnrTG
Cuts at: 0 277 538 1126 1581
Size: 277 261 588 455
Fragments arranged by size:
588 455 277 261
MspA1I CmG'CkG
Cuts at: 0 69 477 1061 1149 1244 1581
Size: 69 408 584 88 95 337
Fragments arranged by size:
584 408 337 95 88 69
NaeI GCC'GGC
Cuts at: 0 209 265 1581
Size: 209 56 1316
Fragments arranged by size:
1316 209 56
NgoMIV G'CCGG_C
Cuts at: 0 207 263 1581
Size: 207 56 1318
Fragments arranged by size:
1318 207 56
NspI r_CATG'y
Cuts at: 0 403 537 909 1389 1581
Size: 403 134 372 480 192
Fragments arranged by size:
480 403 372 192 134
PasI CC'CwG_GG
Cuts at: 0 390 1581
Size: 390 1191
PciI A'CATG_T
Cuts at: 0 905 1385 1581
Size: 905 480 196
Fragments arranged by size:
905 480 196
PflMI CCAn_nnn'nTGG
Cuts at: 0 730 1581
Size: 730 851
PfoI T'CCnGG_A
Cuts at: 0 47 164 326 965 1581
Size: 47 117 162 639 616
Fragments arranged by size:
639 616 162 117 47
PpuMI rG'GwC_Cy
Cuts at: 0 168 1188 1558 1581
Size: 168 1020 370 23
Fragments arranged by size:
1020 370 168 23
PspOMI G'GGCC_C
Cuts at: 0 763 1581
Size: 763 818
PstI C_TGCA'G
Cuts at: 0 17 77 323 1580 1581
Size: 17 60 246 1257 1
Fragments arranged by size:
1257 246 60 17 1
PvuII CAG'CTG
Cuts at: 0 1149 1244 1581
Size: 1149 95 337
Fragments arranged by size:
1149 337 95
SacI G_AGCT'C
Cuts at: 0 373 1581
Size: 373 1208
SanDI GG'GwC_CC
Cuts at: 0 1558 1581
Size: 1558 23
SapI GCTCTTCn'nnn_
Cuts at: 0 1269 1581
Size: 1269 312
ScaI AGT'ACT
Cuts at: 0 1324 1581
Size: 1324 257
SfcI C'TryA_G
Cuts at: 0 13 73 319 465 1576 1581
Size: 13 60 246 146 1111 5
Fragments arranged by size:
1111 246 146 60 13 5
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 133 1581
Size: 133 1448
SmlI C'TyrA_G
Cuts at: 0 361 798 1444 1581
Size: 361 437 646 137
Fragments arranged by size:
646 437 361 137
SphI G_CATG'C
Cuts at: 0 403 537 1581
Size: 403 134 1044
Fragments arranged by size:
1044 403 134
StyI C'CwwG_G
Cuts at: 0 1430 1581
Size: 1430 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1501 1581
Size: 1501 80
TatI w'GTAC_w
Cuts at: 0 83 302 908 917 1130 1322 1581
Size: 83 219 606 9 213 192 259
Fragments arranged by size:
606 259 219 213 192 83 9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 574 627 1581
Size: 574 53 954
Fragments arranged by size:
954 574 53
Enzymes that do cut:
AflIII AhdI AleI AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbsI BbvCI BcgI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstYI BtgI BtgZI BtsI EaeI EagI
EarI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I MmeI
MscI MslI MspA1I NaeI NgoMIV NspI PasI PciI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI
SapI ScaI SfcI SfiI SmlI SphI StyI TaqII
TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BclI BfrBI BmtI BsaAI BsaXI
BsiWI BspEI BspHI BssHII BstBI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EciI EcoNI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI
SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI