Restriction map for
pFN21AE1157
(Linear) (Six-base) MAPSORT of: KIEE1157.seq Check: 6307 from: 1 to: 1695
KIEE1157.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AclI AA'CG_TT
Cuts at: 0 1161 1695
Size: 1161 534
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 425 677 914 1078 1695
Size: 294 131 252 237 164 617
Fragments arranged by size:
617 294 252 237 164 131
AflIII A'CryG_T
Cuts at: 0 1142 1695
Size: 1142 553
AlwNI CAG_nnn'CTG
Cuts at: 0 96 482 795 1695
Size: 96 386 313 900
Fragments arranged by size:
900 386 313 96
ApoI r'AATT_y
Cuts at: 0 220 731 1041 1636 1666 1695
Size: 220 511 310 595 30 29
Fragments arranged by size:
595 511 310 220 30 29
AseI AT'TA_AT
Cuts at: 0 1436 1695
Size: 1436 259
BanII G_rGCy'C
Cuts at: 0 715 1695
Size: 715 980
BbsI GAAGACnn'nnnn_
Cuts at: 0 539 692 1237 1695
Size: 539 153 545 458
Fragments arranged by size:
545 539 458 153
BciVI GTATCCnnnnn_n'
Cuts at: 0 1476 1695
Size: 1476 219
BclI T'GATC_A
Cuts at: 0 786 1695
Size: 786 909
BfrBI ATG'CAT
Cuts at: 0 850 1695
Size: 850 845
BglII A'GATC_T
Cuts at: 0 327 1695
Size: 327 1368
BlpI GC'TnA_GC
Cuts at: 0 1178 1695
Size: 1178 517
BmgBI CAC'GTC
Cuts at: 0 113 610 1695
Size: 113 497 1085
Fragments arranged by size:
1085 497 113
BmrI ACTGGGnnnn_n'
Cuts at: 0 84 1695
Size: 84 1611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 1695
Size: 566 1129
BsaI GGTCTCn'nnnn_
Cuts at: 0 1572 1695
Size: 1572 123
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 517 1695
Size: 517 1178
BsiHKAI G_wGCw'C
Cuts at: 0 1284 1695
Size: 1284 411
BsmI GAATG_Cn'
Cuts at: 0 1132 1695
Size: 1132 563
Bsp1286I G_dGCh'C
Cuts at: 0 715 1284 1695
Size: 715 569 411
Fragments arranged by size:
715 569 411
BspHI T'CATG_A
Cuts at: 0 249 357 1695
Size: 249 108 1338
Fragments arranged by size:
1338 249 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1590 1695
Size: 1590 105
BsrDI GCAATG_nn'
Cuts at: 0 1614 1695
Size: 1614 81
BstYI r'GATC_y
Cuts at: 0 327 898 1695
Size: 327 571 797
Fragments arranged by size:
797 571 327
BtsI GCAGTG_nn'
Cuts at: 0 1352 1695
Size: 1352 343
EarI CTCTTCn'nnn_
Cuts at: 0 272 413 422 1025 1695
Size: 272 141 9 603 670
Fragments arranged by size:
670 603 272 141 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 592 1695
Size: 592 1103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 425 677 914 1078 1695
Size: 294 131 252 237 164 617
Fragments arranged by size:
617 294 252 237 164 131
EcoO109I rG'GnC_Cy
Cuts at: 0 801 1695
Size: 801 894
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 997 1029 1695
Size: 547 32 418 32 666
Fragments arranged by size:
666 547 418 32 32
MslI CAynn'nnrTG
Cuts at: 0 612 1220 1436 1695
Size: 612 608 216 259
Fragments arranged by size:
612 608 259 216
MspA1I CmG'CkG
Cuts at: 0 56 62 235 1695
Size: 56 6 173 1460
Fragments arranged by size:
1460 173 56 6
NdeI CA'TA_TG
Cuts at: 0 1535 1695
Size: 1535 160
NsiI A_TGCA'T
Cuts at: 0 852 1695
Size: 852 843
NspI r_CATG'y
Cuts at: 0 243 351 1146 1695
Size: 243 108 795 549
Fragments arranged by size:
795 549 243 108
PciI A'CATG_T
Cuts at: 0 1142 1695
Size: 1142 553
PflMI CCAn_nnn'nTGG
Cuts at: 0 96 1450 1695
Size: 96 1354 245
Fragments arranged by size:
1354 245 96
PfoI T'CCnGG_A
Cuts at: 0 189 1695
Size: 189 1506
PpuMI rG'GwC_Cy
Cuts at: 0 801 1695
Size: 801 894
PvuII CAG'CTG
Cuts at: 0 235 1695
Size: 235 1460
SanDI GG'GwC_CC
Cuts at: 0 801 1695
Size: 801 894
ScaI AGT'ACT
Cuts at: 0 390 811 1695
Size: 390 421 884
Fragments arranged by size:
884 421 390
SexAI A'CCwGG_T
Cuts at: 0 1386 1695
Size: 1386 309
SfcI C'TryA_G
Cuts at: 0 483 1695
Size: 483 1212
SmlI C'TyrA_G
Cuts at: 0 581 1695
Size: 581 1114
SspI AAT'ATT
Cuts at: 0 622 1397 1695
Size: 622 775 298
Fragments arranged by size:
775 622 298
StuI AGG'CCT
Cuts at: 0 1063 1695
Size: 1063 632
TatI w'GTAC_w
Cuts at: 0 388 809 1695
Size: 388 421 886
Fragments arranged by size:
886 421 388
XbaI T'CTAG_A
Cuts at: 0 853 1248 1695
Size: 853 395 447
Fragments arranged by size:
853 447 395
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 686 1695
Size: 686 1009
XmnI GAAnn'nnTTC
Cuts at: 0 887 1695
Size: 887 808
Enzymes that do cut:
AclI AcuI AflIII AlwNI ApoI AseI BanII BbsI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BpuEI
BsaI BseRI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BstYI BtsI EarI EciI Eco57MI EcoO109I Hin4I MslI
MspA1I NdeI NsiI NspI PciI PflMI PfoI PpuMI
PvuII SanDI ScaI SexAI SfcI SmlI SspI StuI
TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BglI
Bme1580I BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PasI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SapI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StyI SwaI TaqII TstI Tth111I XhoI XmaI ZraI