(Linear) (Six-base) MAPSORT of: KIEE1150.seq  Check: 3072  from: 1  to: 1458



KIEE1150.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1325    1458 
   Size:       1325     133
 
AccI GT'mk_AC

Cuts at:      0    1395    1458 
   Size:       1395      63
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     363    1458 
   Size:        262     101    1095

  Fragments arranged by size: 

                1095     262     101
 
AflII C'TTAA_G

Cuts at:      0     397    1458 
   Size:        397    1061
 
AflIII A'CryG_T

Cuts at:      0     978    1458 
   Size:        978     480
 
AleI CACnn'nnGTG

Cuts at:      0     927    1458 
   Size:        927     531
 
ApaLI G'TGCA_C

Cuts at:      0     472    1458 
   Size:        472     986
 
ApoI r'AATT_y

Cuts at:      0     508     676     873     902    1154    1406    1458 
   Size:        508     168     197      29     252     252      52

  Fragments arranged by size: 

                 508     252     252     197     168      52      29
 
AvaI C'yCGr_G

Cuts at:      0    1047    1458 
   Size:       1047     411
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     369    1204    1458 
   Size:        369     835     254

  Fragments arranged by size: 

                 835     369     254
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     308    1458 
   Size:        308    1150
 
BclI T'GATC_A

Cuts at:      0     891    1458 
   Size:        891     567
 
Bme1580I G_kGCm'C

Cuts at:      0     476    1458 
   Size:        476     982
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1424    1458 
   Size:       1424      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     527    1458 
   Size:        232     295     931

  Fragments arranged by size: 

                 931     295     232
 
BsaWI w'CCGG_w

Cuts at:      0    1330    1458 
   Size:       1330     128
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     525     555    1458 
   Size:        525      30     903

  Fragments arranged by size: 

                 903     525      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172    1458 
   Size:        172    1286
 
BsiHKAI G_wGCw'C

Cuts at:      0     476    1458 
   Size:        476     982
 
BsmI GAATG_Cn'

Cuts at:      0    1438    1458 
   Size:       1438      20
 
Bsp1286I G_dGCh'C

Cuts at:      0     476    1458 
   Size:        476     982
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1325    1458 
   Size:       1325     133
 
BsrGI T'GTAC_A

Cuts at:      0     555    1458 
   Size:        555     903
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     161    1458 
   Size:        161    1297
 
BstYI r'GATC_y

Cuts at:      0     862    1458 
   Size:        862     596
 
BtgI C'CryG_G

Cuts at:      0      21    1458 
   Size:         21    1437
 
BtsI GCAGTG_nn'

Cuts at:      0     730    1458 
   Size:        730     728
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1257    1292    1458 
   Size:       1257      35     166

  Fragments arranged by size: 

                1257     166      35
 
EaeI y'GGCC_r

Cuts at:      0    1026    1458 
   Size:       1026     432
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     262     363     527    1458 
   Size:        232      30     101     164     931

  Fragments arranged by size: 

                 931     232     164     101      30
 
EcoRI G'AATT_C

Cuts at:      0    1154    1406    1458 
   Size:       1154     252      52

  Fragments arranged by size: 

                1154     252      52
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     332     364    1458 
   Size:        332      32    1094

  Fragments arranged by size: 

                1094     332      32
 
MscI TGG'CCA

Cuts at:      0    1028    1458 
   Size:       1028     430
 
MslI CAynn'nnrTG

Cuts at:      0     659     927    1458 
   Size:        659     268     531

  Fragments arranged by size: 

                 659     531     268
 
NdeI CA'TA_TG

Cuts at:      0     644    1458 
   Size:        644     814
 
NspI r_CATG'y

Cuts at:      0     982    1458 
   Size:        982     476
 
PciI A'CATG_T

Cuts at:      0     978    1458 
   Size:        978     480
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     217     249    1458 
   Size:        217      32    1209

  Fragments arranged by size: 

                1209     217      32
 
ScaI AGT'ACT

Cuts at:      0    1045    1458 
   Size:       1045     413
 
SfcI C'TryA_G

Cuts at:      0     195     333    1458 
   Size:        195     138    1125

  Fragments arranged by size: 

                1125     195     138
 
SmlI C'TyrA_G

Cuts at:      0     397    1458 
   Size:        397    1061
 
SspI AAT'ATT

Cuts at:      0     305    1458 
   Size:        305    1153
 
TatI w'GTAC_w

Cuts at:      0     555     931    1043    1458 
   Size:        555     376     112     415

  Fragments arranged by size: 

                 555     415     376     112
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     336     368    1458 
   Size:        336      32    1090

  Fragments arranged by size: 

                1090     336      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     781     949    1117    1201    1342    1406    1458 
   Size:        781     168     168      84     141      64      52

  Fragments arranged by size: 

                 781     168     168     141      84      64      52
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AleI    ApaLI     ApoI
     AvaI     BbsI    BciVI     BclI Bme1580I     BmrI     BpmI    BsaWI
    BsaXI    BseRI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrGI    BstXI
    BstYI     BtgI     BtsI    CspCI     EaeI  Eco57MI    EcoRI    Hin4I
     MscI     MslI     NdeI     NspI     PciI     PpiI     ScaI     SfcI
     SmlI     SspI     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AlfI     AloI
    AlwNI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI