Restriction map for
pFN21AE1148
(Linear) (Six-base) MAPSORT of: KIEE1148.seq Check: 4866 from: 1 to: 1386
KIEE1148.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 149 1386
Size: 149 1237
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 1386
Size: 462 924
AfeI AGC'GCT
Cuts at: 0 152 1386
Size: 152 1234
AleI CACnn'nnGTG
Cuts at: 0 698 1386
Size: 698 688
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 275 589 1386
Size: 275 314 797
Fragments arranged by size:
797 314 275
AlwNI CAG_nnn'CTG
Cuts at: 0 141 837 909 966 1255 1386
Size: 141 696 72 57 289 131
Fragments arranged by size:
696 289 141 131 72 57
ApaI G_GGCC'C
Cuts at: 0 8 201 247 529 1029 1155 1179 1386
Size: 8 193 46 282 500 126 24 207
Fragments arranged by size:
500 282 207 193 126 46 24 8
ApaLI G'TGCA_C
Cuts at: 0 859 1386
Size: 859 527
AvaI C'yCGr_G
Cuts at: 0 848 983 1386
Size: 848 135 403
Fragments arranged by size:
848 403 135
AvrII C'CTAG_G
Cuts at: 0 736 1386
Size: 736 650
BanII G_rGCy'C
Cuts at: 0 8 201 247 529 746 1029 1155 1179
Size: 8 193 46 282 217 283 126 24
Cuts at: 1179 1386
Size: 207
Fragments arranged by size:
283 282 217 207 193 126 46 24
8
BbvCI CC'TCA_GC
Cuts at: 0 457 971 1157 1386
Size: 457 514 186 229
Fragments arranged by size:
514 457 229 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 14 48 1386
Size: 14 34 1338
Fragments arranged by size:
1338 34 14
BglI GCCn_nnn'nGGC
Cuts at: 0 759 833 1120 1150 1386
Size: 759 74 287 30 236
Fragments arranged by size:
759 287 236 74 30
BlpI GC'TnA_GC
Cuts at: 0 147 512 1386
Size: 147 365 874
Fragments arranged by size:
874 365 147
Bme1580I G_kGCm'C
Cuts at: 0 8 119 201 247 529 863 1029 1155
Size: 8 111 82 46 282 334 166 126
Cuts at: 1155 1179 1386
Size: 24 207
Fragments arranged by size:
334 282 207 166 126 111 82 46
24 8
BmgBI CAC'GTC
Cuts at: 0 276 1386
Size: 276 1110
BmrI ACTGGGnnnn_n'
Cuts at: 0 1024 1182 1386
Size: 1024 158 204
Fragments arranged by size:
1024 204 158
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 424 943 1386
Size: 202 222 519 443
Fragments arranged by size:
519 443 222 202
Bpu10I CC'TnA_GC
Cuts at: 0 457 971 1157 1386
Size: 457 514 186 229
Fragments arranged by size:
514 457 229 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 1386
Size: 250 1136
BsaI GGTCTCn'nnnn_
Cuts at: 0 343 781 1386
Size: 343 438 605
Fragments arranged by size:
605 438 343
BsaAI yAC'GTr
Cuts at: 0 1200 1386
Size: 1200 186
BsaHI Gr'CG_yC
Cuts at: 0 1008 1386
Size: 1008 378
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 733 763 1386
Size: 175 30 528 30 623
Fragments arranged by size:
623 528 175 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1121 1386
Size: 1121 265
BseYI C'CCAG_C
Cuts at: 0 207 522 528 840 977 1148 1230 1248
Size: 207 315 6 312 137 171 82 18
Cuts at: 1248 1258 1386
Size: 10 128
Fragments arranged by size:
315 312 207 171 137 128 82 18
10 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 1051 1386
Size: 315 736 335
Fragments arranged by size:
736 335 315
BsiHKAI G_wGCw'C
Cuts at: 0 513 611 683 863 1037 1386
Size: 513 98 72 180 174 349
Fragments arranged by size:
513 349 180 174 98 72
BsmI GAATG_Cn'
Cuts at: 0 694 1386
Size: 694 692
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1065 1386
Size: 1065 321
Bsp1286I G_dGCh'C
Cuts at: 0 8 119 201 247 513 529 611 683
Size: 8 111 82 46 266 16 82 72
Cuts at: 683 746 863 1029 1037 1155 1179 1386
Size: 63 117 166 8 118 24 207
Fragments arranged by size:
266 207 166 118 117 111 82 82
72 63 46 24 16 8 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 149 1386
Size: 149 1237
BsrDI GCAATG_nn'
Cuts at: 0 422 1386
Size: 422 964
BsrFI r'CCGG_y
Cuts at: 0 463 1386
Size: 463 923
BssSI C'ACGA_G
Cuts at: 0 23 377 1386
Size: 23 354 1009
Fragments arranged by size:
1009 354 23
BstAPI GCAn_nnn'nTGC
Cuts at: 0 141 1386
Size: 141 1245
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1362 1386
Size: 1362 24
Bsu36I CC'TnA_GG
Cuts at: 0 166 1386
Size: 166 1220
BtgI C'CryG_G
Cuts at: 0 247 344 757 1347 1386
Size: 247 97 413 590 39
Fragments arranged by size:
590 413 247 97 39
BtsI GCAGTG_nn'
Cuts at: 0 1092 1386
Size: 1092 294
DrdI GACnn_nn'nnGTC
Cuts at: 0 1366 1386
Size: 1366 20
EaeI y'GGCC_r
Cuts at: 0 50 1386
Size: 50 1336
EarI CTCTTCn'nnn_
Cuts at: 0 1373 1386
Size: 1373 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 424 462 943 1386
Size: 202 222 38 481 443
Fragments arranged by size:
481 443 222 202 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 702 1386
Size: 702 684
EcoO109I rG'GnC_Cy
Cuts at: 0 4 177 198 243 834 844 882 1025
Size: 4 173 21 45 591 10 38 143
Cuts at: 1025 1026 1113 1152 1262 1270 1293 1386
Size: 1 87 39 110 8 23 93
Fragments arranged by size:
591 173 143 110 93 87 45 39
38 23 21 10 8 4 1
HaeII r_GCGC'y
Cuts at: 0 154 932 1005 1185 1329 1386
Size: 154 778 73 180 144 57
Fragments arranged by size:
778 180 154 144 73 57
MscI TGG'CCA
Cuts at: 0 52 1386
Size: 52 1334
MslI CAynn'nnrTG
Cuts at: 0 698 1386
Size: 698 688
MspA1I CmG'CkG
Cuts at: 0 174 249 516 780 873 1148 1386
Size: 174 75 267 264 93 275 238
Fragments arranged by size:
275 267 264 238 174 93 75
NcoI C'CATG_G
Cuts at: 0 344 1386
Size: 344 1042
NspI r_CATG'y
Cuts at: 0 644 1386
Size: 644 742
PasI CC'CwG_GG
Cuts at: 0 1125 1386
Size: 1125 261
PfoI T'CCnGG_A
Cuts at: 0 65 611 1386
Size: 65 546 775
Fragments arranged by size:
775 546 65
PpuMI rG'GwC_Cy
Cuts at: 0 177 844 882 1262 1386
Size: 177 667 38 380 124
Fragments arranged by size:
667 380 177 124 38
PshAI GACnn'nnGTC
Cuts at: 0 274 1217 1386
Size: 274 943 169
Fragments arranged by size:
943 274 169
PspOMI G'GGCC_C
Cuts at: 0 4 197 243 525 1025 1151 1175 1386
Size: 4 193 46 282 500 126 24 211
Fragments arranged by size:
500 282 211 193 126 46 24 4
PstI C_TGCA'G
Cuts at: 0 582 806 928 1386
Size: 582 224 122 458
Fragments arranged by size:
582 458 224 122
PvuII CAG'CTG
Cuts at: 0 516 780 1386
Size: 516 264 606
Fragments arranged by size:
606 516 264
SacII CC_GC'GG
Cuts at: 0 250 1386
Size: 250 1136
SanDI GG'GwC_CC
Cuts at: 0 882 1262 1386
Size: 882 380 124
Fragments arranged by size:
882 380 124
SapI GCTCTTCn'nnn_
Cuts at: 0 1373 1386
Size: 1373 13
SfcI C'TryA_G
Cuts at: 0 578 802 924 1386
Size: 578 224 122 462
Fragments arranged by size:
578 462 224 122
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1120 1386
Size: 1120 266
SmlI C'TyrA_G
Cuts at: 0 265 1386
Size: 265 1121
StuI AGG'CCT
Cuts at: 0 735 812 1386
Size: 735 77 574
Fragments arranged by size:
735 574 77
StyI C'CwwG_G
Cuts at: 0 344 736 1386
Size: 344 392 650
Fragments arranged by size:
650 392 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 106 878 1057 1335 1386
Size: 106 772 179 278 51
Fragments arranged by size:
772 278 179 106 51
TatI w'GTAC_w
Cuts at: 0 488 1386
Size: 488 898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 63 1386
Size: 63 1323
XmnI GAAnn'nnTTC
Cuts at: 0 475 1386
Size: 475 911
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI AlwNI ApaI ApaLI
AvaI AvrII BanII BbvCI BcgI BglI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrDI BsrFI BssSI BstAPI BstXI Bsu36I BtgI
BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I HaeII
MscI MslI MspA1I NcoI NspI PasI PfoI PpuMI
PshAI PspOMI PstI PvuII SacII SanDI SapI SfcI
SfiI SmlI StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI ApoI AscI AseI AsiSI BaeI BamHI BanI
BbeI BbsI BciVI BclI BfrBI BglII BmtI BplI
BsaBI BsaWI BsiEI BsiWI BspEI BspHI BsrBI BsrGI
BssHII BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI
DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI
SalI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI