Restriction map for
pFN21AE1143
(Linear) (Six-base) MAPSORT of: KIEE1143.seq Check: 6542 from: 1 to: 1275
KIEE1143.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AatII G_ACGT'C
Cuts at: 0 68 1275
Size: 68 1207
AccI GT'mk_AC
Cuts at: 0 62 1275
Size: 62 1213
AclI AA'CG_TT
Cuts at: 0 561 1275
Size: 561 714
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 847 1275
Size: 452 395 428
Fragments arranged by size:
452 428 395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 518 1275
Size: 518 757
ApoI r'AATT_y
Cuts at: 0 218 285 321 360 1275
Size: 218 67 36 39 915
Fragments arranged by size:
915 218 67 39 36
BanI G'GyrC_C
Cuts at: 0 16 502 1275
Size: 16 486 773
Fragments arranged by size:
773 486 16
BanII G_rGCy'C
Cuts at: 0 47 154 1275
Size: 47 107 1121
Fragments arranged by size:
1121 107 47
BbeI G_GCGC'C
Cuts at: 0 20 1275
Size: 20 1255
BbsI GAAGACnn'nnnn_
Cuts at: 0 440 482 1144 1275
Size: 440 42 662 131
Fragments arranged by size:
662 440 131 42
BciVI GTATCCnnnnn_n'
Cuts at: 0 1068 1275
Size: 1068 207
BclI T'GATC_A
Cuts at: 0 657 1275
Size: 657 618
BglII A'GATC_T
Cuts at: 0 972 1275
Size: 972 303
BlpI GC'TnA_GC
Cuts at: 0 878 1275
Size: 878 397
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 153 1275
Size: 153 1122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 109 790 1275
Size: 61 48 681 485
Fragments arranged by size:
681 485 61 48
Bpu10I CC'TnA_GC
Cuts at: 0 1081 1275
Size: 1081 194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 1144 1275
Size: 91 1053 131
Fragments arranged by size:
1053 131 91
BsaI GGTCTCn'nnnn_
Cuts at: 0 408 1275
Size: 408 867
BsaHI Gr'CG_yC
Cuts at: 0 17 65 1275
Size: 17 48 1210
Fragments arranged by size:
1210 48 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 97 1275
Size: 88 9 1178
Fragments arranged by size:
1178 88 9
BseYI C'CCAG_C
Cuts at: 0 22 1275
Size: 22 1253
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 572 1275
Size: 174 398 703
Fragments arranged by size:
703 398 174
BsiHKAI G_wGCw'C
Cuts at: 0 139 1275
Size: 139 1136
BsmI GAATG_Cn'
Cuts at: 0 334 1275
Size: 334 941
Bsp1286I G_dGCh'C
Cuts at: 0 47 139 154 1275
Size: 47 92 15 1121
Fragments arranged by size:
1121 92 47 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 196 952 1275
Size: 196 756 323
Fragments arranged by size:
756 323 196
BsrFI r'CCGG_y
Cuts at: 0 781 1275
Size: 781 494
BstAPI GCAn_nnn'nTGC
Cuts at: 0 175 1275
Size: 175 1100
BstYI r'GATC_y
Cuts at: 0 972 1275
Size: 972 303
BtgI C'CryG_G
Cuts at: 0 47 486 1275
Size: 47 439 789
Fragments arranged by size:
789 439 47
BtsI GCAGTG_nn'
Cuts at: 0 899 1275
Size: 899 376
DraI TTT'AAA
Cuts at: 0 318 1275
Size: 318 957
EaeI y'GGCC_r
Cuts at: 0 2 1275
Size: 2 1273
EarI CTCTTCn'nnn_
Cuts at: 0 1214 1239 1275
Size: 1214 25 36
Fragments arranged by size:
1214 36 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 109 452 790 847 1275
Size: 61 48 343 338 57 428
Fragments arranged by size:
428 343 338 61 57 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 73 709 1275
Size: 73 636 566
Fragments arranged by size:
636 566 73
HaeII r_GCGC'y
Cuts at: 0 20 1275
Size: 20 1255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 405 437 1213 1245 1275
Size: 405 32 776 32 30
Fragments arranged by size:
776 405 32 32 30
HincII GTy'rAC
Cuts at: 0 63 1275
Size: 63 1212
KasI G'GCGC_C
Cuts at: 0 16 1275
Size: 16 1259
MfeI C'AATT_G
Cuts at: 0 592 1275
Size: 592 683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 1275
Size: 14 1261
MslI CAynn'nnrTG
Cuts at: 0 500 1275
Size: 500 775
MspA1I CmG'CkG
Cuts at: 0 22 1275
Size: 22 1253
NaeI GCC'GGC
Cuts at: 0 783 1275
Size: 783 492
NarI GG'CG_CC
Cuts at: 0 17 1275
Size: 17 1258
NcoI C'CATG_G
Cuts at: 0 486 1275
Size: 486 789
NgoMIV G'CCGG_C
Cuts at: 0 781 1275
Size: 781 494
PflMI CCAn_nnn'nTGG
Cuts at: 0 911 1107 1275
Size: 911 196 168
Fragments arranged by size:
911 196 168
PfoI T'CCnGG_A
Cuts at: 0 743 1275
Size: 743 532
PsiI TTA'TAA
Cuts at: 0 889 1275
Size: 889 386
PstI C_TGCA'G
Cuts at: 0 1273 1275
Size: 1273 2
SalI G'TCGA_C
Cuts at: 0 61 1275
Size: 61 1214
SfcI C'TryA_G
Cuts at: 0 180 1269 1275
Size: 180 1089 6
Fragments arranged by size:
1089 180 6
SfoI GGC'GCC
Cuts at: 0 18 1275
Size: 18 1257
SmlI C'TyrA_G
Cuts at: 0 70 1159 1275
Size: 70 1089 116
Fragments arranged by size:
1089 116 70
StuI AGG'CCT
Cuts at: 0 550 1275
Size: 550 725
StyI C'CwwG_G
Cuts at: 0 486 1275
Size: 486 789
TatI w'GTAC_w
Cuts at: 0 374 398 1275
Size: 374 24 877
Fragments arranged by size:
877 374 24
XbaI T'CTAG_A
Cuts at: 0 975 1275
Size: 975 300
XmnI GAAnn'nnTTC
Cuts at: 0 1223 1275
Size: 1223 52
ZraI GAC'GTC
Cuts at: 0 66 1275
Size: 66 1209
Enzymes that do cut:
AatII AccI AclI AcuI AlfI ApoI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrFI BstAPI BstYI BtgI BtsI
DraI EaeI EarI Eco57MI EcoNI HaeII Hin4I HincII
KasI MfeI MmeI MslI MspA1I NaeI NarI NcoI
NgoMIV PflMI PfoI PsiI PstI SalI SfcI SfoI
SmlI StuI StyI TatI XbaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AflIII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbvCI BcgI BfrBI BglI Bme1580I
BmgBI BmrI BmtI BsaAI BsaBI BsaWI BsaXI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MluI MscI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TstI Tth111I XcmI XhoI XmaI