Restriction map for
pFN21AE1138
(Linear) (Six-base) MAPSORT of: KIEE1138.seq Check: 9977 from: 1 to: 1116
KIEE1138.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 1116
Size: 434 682
AleI CACnn'nnGTG
Cuts at: 0 358 1116
Size: 358 758
AlwNI CAG_nnn'CTG
Cuts at: 0 90 1116
Size: 90 1026
ApoI r'AATT_y
Cuts at: 0 826 1116
Size: 826 290
BanI G'GyrC_C
Cuts at: 0 360 1116
Size: 360 756
BanII G_rGCy'C
Cuts at: 0 191 1116
Size: 191 925
Bme1580I G_kGCm'C
Cuts at: 0 162 1116
Size: 162 954
BmgBI CAC'GTC
Cuts at: 0 207 1116
Size: 207 909
BmrI ACTGGGnnnn_n'
Cuts at: 0 434 1116
Size: 434 682
BsaHI Gr'CG_yC
Cuts at: 0 4 536 1116
Size: 4 532 580
Fragments arranged by size:
580 532 4
BsaWI w'CCGG_w
Cuts at: 0 174 738 1116
Size: 174 564 378
Fragments arranged by size:
564 378 174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 624 654 883 913 1116
Size: 624 30 229 30 203
Fragments arranged by size:
624 229 203 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 499 502 505 514 517 904 1116
Size: 499 3 3 9 3 387 212
Fragments arranged by size:
499 387 212 9 3 3 3
BsiHKAI G_wGCw'C
Cuts at: 0 97 191 575 1116
Size: 97 94 384 541
Fragments arranged by size:
541 384 97 94
BsmI GAATG_Cn'
Cuts at: 0 1047 1116
Size: 1047 69
Bsp1286I G_dGCh'C
Cuts at: 0 97 162 191 575 1116
Size: 97 65 29 384 541
Fragments arranged by size:
541 384 97 65 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 837 1116
Size: 837 279
BsrBI CCG'CTC
Cuts at: 0 549 1116
Size: 549 567
Bsu36I CC'TnA_GG
Cuts at: 0 365 761 1116
Size: 365 396 355
Fragments arranged by size:
396 365 355
BtgI C'CryG_G
Cuts at: 0 725 1116
Size: 725 391
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1067 1116
Size: 1067 49
ClaI AT'CG_AT
Cuts at: 0 923 1116
Size: 923 193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 384 419 1116
Size: 384 35 697
Fragments arranged by size:
697 384 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 334 1116
Size: 334 782
EaeI y'GGCC_r
Cuts at: 0 236 1116
Size: 236 880
EarI CTCTTCn'nnn_
Cuts at: 0 491 591 875 1031 1116
Size: 491 100 284 156 85
Fragments arranged by size:
491 284 156 100 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 6 267 1116
Size: 6 261 849
Fragments arranged by size:
849 261 6
EcoICRI GAG'CTC
Cuts at: 0 189 1116
Size: 189 927
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 1116
Size: 434 682
EcoNI CCTnn'n_nnAGG
Cuts at: 0 665 1116
Size: 665 451
EcoO109I rG'GnC_Cy
Cuts at: 0 648 1116
Size: 648 468
EcoRI G'AATT_C
Cuts at: 0 826 1116
Size: 826 290
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 372 1116
Size: 372 744
HaeII r_GCGC'y
Cuts at: 0 292 316 1116
Size: 292 24 800
Fragments arranged by size:
800 292 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 84 102 116 134 1066 1098 1116
Size: 84 18 14 18 932 32 18
Fragments arranged by size:
932 84 32 18 18 18 14
HincII GTy'rAC
Cuts at: 0 852 1116
Size: 852 264
MfeI C'AATT_G
Cuts at: 0 674 1116
Size: 674 442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 578 881 1116
Size: 82 496 303 235
Fragments arranged by size:
496 303 235 82
MslI CAynn'nnrTG
Cuts at: 0 358 387 1116
Size: 358 29 729
Fragments arranged by size:
729 358 29
MspA1I CmG'CkG
Cuts at: 0 150 727 1116
Size: 150 577 389
Fragments arranged by size:
577 389 150
NruI TCG'CGA
Cuts at: 0 127 1116
Size: 127 989
PshAI GACnn'nnGTC
Cuts at: 0 205 1116
Size: 205 911
PstI C_TGCA'G
Cuts at: 0 1025 1116
Size: 1025 91
PvuII CAG'CTG
Cuts at: 0 150 1116
Size: 150 966
SacI G_AGCT'C
Cuts at: 0 191 1116
Size: 191 925
SacII CC_GC'GG
Cuts at: 0 728 1116
Size: 728 388
ScaI AGT'ACT
Cuts at: 0 633 1116
Size: 633 483
SfcI C'TryA_G
Cuts at: 0 1021 1069 1116
Size: 1021 48 47
Fragments arranged by size:
1021 48 47
StyI C'CwwG_G
Cuts at: 0 356 644 1116
Size: 356 288 472
Fragments arranged by size:
472 356 288
TatI w'GTAC_w
Cuts at: 0 131 631 1116
Size: 131 500 485
Fragments arranged by size:
500 485 131
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 565 597 802 834 1116
Size: 565 32 205 32 282
Fragments arranged by size:
565 282 205 32 32
Enzymes that do cut:
AcuI AleI AlwNI ApoI BanI BanII Bme1580I BmgBI
BmrI BsaHI BsaWI BsaXI BseRI BsiHKAI BsmI Bsp1286I
BspMI BsrBI Bsu36I BtgI BtgZI ClaI CspCI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII MfeI MmeI MslI MspA1I
NruI PshAI PstI PvuII SacI SacII ScaI SfcI
StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BseYI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtsI DraI DraIII EagI EcoRV FseI FspI
FspAI HindIII HpaI KasI KpnI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI