Restriction map for
pFN21AE1135
(Linear) (Six-base) MAPSORT of: KIEE1135.seq Check: 9809 from: 1 to: 1059
KIEE1135.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AccI GT'mk_AC
Cuts at: 0 1019 1059
Size: 1019 40
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 1059
Size: 93 966
AflIII A'CryG_T
Cuts at: 0 480 1059
Size: 480 579
AhdI GACnn_n'nnGTC
Cuts at: 0 461 1059
Size: 461 598
AlwNI CAG_nnn'CTG
Cuts at: 0 445 564 774 814 849 1059
Size: 445 119 210 40 35 210
Fragments arranged by size:
445 210 210 119 40 35
ApaI G_GGCC'C
Cuts at: 0 42 145 1048 1059
Size: 42 103 903 11
Fragments arranged by size:
903 103 42 11
AvaI C'yCGr_G
Cuts at: 0 64 119 1025 1059
Size: 64 55 906 34
Fragments arranged by size:
906 64 55 34
BanI G'GyrC_C
Cuts at: 0 68 159 667 735 862 873 944 950
Size: 68 91 508 68 127 11 71 6
Cuts at: 950 967 1059
Size: 17 92
Fragments arranged by size:
508 127 92 91 71 68 68 17
11 6
BanII G_rGCy'C
Cuts at: 0 42 145 838 1048 1059
Size: 42 103 693 210 11
Fragments arranged by size:
693 210 103 42 11
BbeI G_GCGC'C
Cuts at: 0 739 954 1059
Size: 739 215 105
Fragments arranged by size:
739 215 105
BbvCI CC'TCA_GC
Cuts at: 0 343 815 1059
Size: 343 472 244
Fragments arranged by size:
472 343 244
BglI GCCn_nnn'nGGC
Cuts at: 0 353 1059
Size: 353 706
Bme1580I G_kGCm'C
Cuts at: 0 42 145 164 477 521 599 865 878
Size: 42 103 19 313 44 78 266 13
Cuts at: 878 970 1048 1059
Size: 92 78 11
Fragments arranged by size:
313 266 103 92 78 78 44 42
19 13 11
BmgBI CAC'GTC
Cuts at: 0 405 1059
Size: 405 654
BmrI ACTGGGnnnn_n'
Cuts at: 0 734 813 1059
Size: 734 79 246
Fragments arranged by size:
734 246 79
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 349 975 1059
Size: 229 120 626 84
Fragments arranged by size:
626 229 120 84
Bpu10I CC'TnA_GC
Cuts at: 0 343 397 412 628 728 815 832 1059
Size: 343 54 15 216 100 87 17 227
Fragments arranged by size:
343 227 216 100 87 54 17 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 79 441 1059
Size: 79 362 618
Fragments arranged by size:
618 362 79
BsaAI yAC'GTr
Cuts at: 0 483 1059
Size: 483 576
BsaHI Gr'CG_yC
Cuts at: 0 736 951 1059
Size: 736 215 108
Fragments arranged by size:
736 215 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 181 211 803 833 1059
Size: 181 30 592 30 226
Fragments arranged by size:
592 226 181 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 886 1059
Size: 886 173
BseYI C'CCAG_C
Cuts at: 0 681 1059
Size: 681 378
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 994 1059
Size: 994 65
BsiHKAI G_wGCw'C
Cuts at: 0 838 1059
Size: 838 221
Bsp1286I G_dGCh'C
Cuts at: 0 42 145 164 477 521 599 838 865
Size: 42 103 19 313 44 78 239 27
Cuts at: 865 878 970 1048 1059
Size: 13 92 78 11
Fragments arranged by size:
313 239 103 92 78 78 44 42
27 19 13 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 290 759 1059
Size: 290 469 300
Fragments arranged by size:
469 300 290
BsrFI r'CCGG_y
Cuts at: 0 156 203 535 581 947 1059
Size: 156 47 332 46 366 112
Fragments arranged by size:
366 332 156 112 47 46
BssSI C'ACGA_G
Cuts at: 0 514 528 1059
Size: 514 14 531
Fragments arranged by size:
531 514 14
BstXI CCAn_nnnn'nTGG
Cuts at: 0 171 207 1059
Size: 171 36 852
Fragments arranged by size:
852 171 36
BstYI r'GATC_y
Cuts at: 0 267 1059
Size: 267 792
Bsu36I CC'TnA_GG
Cuts at: 0 884 1059
Size: 884 175
BtgI C'CryG_G
Cuts at: 0 4 1059
Size: 4 1055
BtsI GCAGTG_nn'
Cuts at: 0 302 910 1011 1059
Size: 302 608 101 48
Fragments arranged by size:
608 302 101 48
EaeI y'GGCC_r
Cuts at: 0 26 52 707 1059
Size: 26 26 655 352
Fragments arranged by size:
655 352 26 26
EcoICRI GAG'CTC
Cuts at: 0 836 1059
Size: 836 223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 229 349 975 1059
Size: 93 136 120 626 84
Fragments arranged by size:
626 136 120 93 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 883 1059
Size: 883 176
EcoO109I rG'GnC_Cy
Cuts at: 0 38 142 771 1044 1045 1059
Size: 38 104 629 273 1 14
Fragments arranged by size:
629 273 104 38 14 1
FseI GG_CCGG'CC
Cuts at: 0 539 1059
Size: 539 520
HaeII r_GCGC'y
Cuts at: 0 739 892 954 1059
Size: 739 153 62 105
Fragments arranged by size:
739 153 105 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 447 479 1059
Size: 181 32 234 32 580
Fragments arranged by size:
580 234 181 32 32
HindIII A'AGCT_T
Cuts at: 0 796 1059
Size: 796 263
KasI G'GCGC_C
Cuts at: 0 735 950 1059
Size: 735 215 109
Fragments arranged by size:
735 215 109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1059
Size: 224 835
MscI TGG'CCA
Cuts at: 0 709 1059
Size: 709 350
MslI CAynn'nnrTG
Cuts at: 0 157 1059
Size: 157 902
NaeI GCC'GGC
Cuts at: 0 537 949 1059
Size: 537 412 110
Fragments arranged by size:
537 412 110
NarI GG'CG_CC
Cuts at: 0 736 951 1059
Size: 736 215 108
Fragments arranged by size:
736 215 108
NgoMIV G'CCGG_C
Cuts at: 0 535 947 1059
Size: 535 412 112
Fragments arranged by size:
535 412 112
PfoI T'CCnGG_A
Cuts at: 0 428 773 1059
Size: 428 345 286
Fragments arranged by size:
428 345 286
PmlI CAC'GTG
Cuts at: 0 483 1059
Size: 483 576
PpuMI rG'GwC_Cy
Cuts at: 0 771 1059
Size: 771 288
PspOMI G'GGCC_C
Cuts at: 0 38 141 1044 1059
Size: 38 103 903 15
Fragments arranged by size:
903 103 38 15
PstI C_TGCA'G
Cuts at: 0 719 770 845 917 1059
Size: 719 51 75 72 142
Fragments arranged by size:
719 142 75 72 51
SacI G_AGCT'C
Cuts at: 0 838 1059
Size: 838 221
SbfI CC_TGCA'GG
Cuts at: 0 770 845 1059
Size: 770 75 214
Fragments arranged by size:
770 214 75
SexAI A'CCwGG_T
Cuts at: 0 869 1059
Size: 869 190
SfcI C'TryA_G
Cuts at: 0 715 766 841 913 1059
Size: 715 51 75 72 146
Fragments arranged by size:
715 146 75 72 51
SfoI GGC'GCC
Cuts at: 0 737 952 1059
Size: 737 215 107
Fragments arranged by size:
737 215 107
SmaI CCC'GGG
Cuts at: 0 66 1027 1059
Size: 66 961 32
Fragments arranged by size:
961 66 32
StuI AGG'CCT
Cuts at: 0 689 830 984 1059
Size: 689 141 154 75
Fragments arranged by size:
689 154 141 75
StyI C'CwwG_G
Cuts at: 0 998 1059
Size: 998 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 315 1059
Size: 315 744
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 803 835 928 960 1059
Size: 803 32 93 32 99
Fragments arranged by size:
803 99 93 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 999 1059
Size: 999 60
XmaI C'CCGG_G
Cuts at: 0 64 1025 1059
Size: 64 961 34
Fragments arranged by size:
961 64 34
Enzymes that do cut:
AccI AcuI AflIII AhdI AlwNI ApaI AvaI BanI
BanII BbeI BbvCI BglI Bme1580I BmgBI BmrI BpmI
Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrFI BssSI BstXI BstYI Bsu36I
BtgI BtsI EaeI EcoICRI Eco57MI EcoNI EcoO109I FseI
HaeII Hin4I HindIII KasI MmeI MscI MslI NaeI
NarI NgoMIV PfoI PmlI PpuMI PspOMI PstI SacI
SbfI SexAI SfcI SfoI SmaI StuI StyI TaqII
TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BplI BpuEI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoRI EcoRV FalI
FspI FspAI HincII HpaI KpnI MfeI MluI MspA1I
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI
ZraI