Restriction map for
pFN21AE1124
(Linear) (Six-base) MAPSORT of: KIEE1124.seq Check: 2045 from: 1 to: 1407
KIEE1124.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1029 1407
Size: 1029 378
Acc65I G'GTAC_C
Cuts at: 0 818 1407
Size: 818 589
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 1407
Size: 926 481
AhdI GACnn_n'nnGTC
Cuts at: 0 465 1407
Size: 465 942
AleI CACnn'nnGTG
Cuts at: 0 391 1407
Size: 391 1016
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 370 1407
Size: 370 1037
AlwNI CAG_nnn'CTG
Cuts at: 0 888 1011 1197 1407
Size: 888 123 186 210
Fragments arranged by size:
888 210 186 123
ApaLI G'TGCA_C
Cuts at: 0 1205 1407
Size: 1205 202
ApoI r'AATT_y
Cuts at: 0 793 1407
Size: 793 614
AvaI C'yCGr_G
Cuts at: 0 328 580 717 1381 1407
Size: 328 252 137 664 26
Fragments arranged by size:
664 328 252 137 26
BamHI G'GATC_C
Cuts at: 0 147 1407
Size: 147 1260
BanI G'GyrC_C
Cuts at: 0 402 553 818 1113 1305 1407
Size: 402 151 265 295 192 102
Fragments arranged by size:
402 295 265 192 151 102
BanII G_rGCy'C
Cuts at: 0 58 983 1407
Size: 58 925 424
Fragments arranged by size:
925 424 58
BbeI G_GCGC'C
Cuts at: 0 1309 1407
Size: 1309 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 53 107 422 1070 1407
Size: 53 54 315 648 337
Fragments arranged by size:
648 337 315 54 53
BglI GCCn_nnn'nGGC
Cuts at: 0 235 1407
Size: 235 1172
Bme1580I G_kGCm'C
Cuts at: 0 1209 1335 1407
Size: 1209 126 72
Fragments arranged by size:
1209 126 72
BmgBI CAC'GTC
Cuts at: 0 344 1407
Size: 344 1063
BmrI ACTGGGnnnn_n'
Cuts at: 0 61 1407
Size: 61 1346
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 462 1407
Size: 462 945
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1147 1252 1407
Size: 253 894 105 155
Fragments arranged by size:
894 253 155 105
Bpu10I CC'TnA_GC
Cuts at: 0 52 1407
Size: 52 1355
BsaAI yAC'GTr
Cuts at: 0 1176 1296 1407
Size: 1176 120 111
Fragments arranged by size:
1176 120 111
BsaHI Gr'CG_yC
Cuts at: 0 375 1306 1407
Size: 375 931 101
Fragments arranged by size:
931 375 101
BsaWI w'CCGG_w
Cuts at: 0 773 1407
Size: 773 634
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 913 943 951 981 1407
Size: 913 30 8 30 426
Fragments arranged by size:
913 426 30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1218 1407
Size: 1218 189
BseYI C'CCAG_C
Cuts at: 0 561 1407
Size: 561 846
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 718 997 1063 1318 1407
Size: 607 111 279 66 255 89
Fragments arranged by size:
607 279 255 111 89 66
BsiEI CG_ry'CG
Cuts at: 0 1328 1343 1407
Size: 1328 15 64
Fragments arranged by size:
1328 64 15
BsiHKAI G_wGCw'C
Cuts at: 0 961 983 1209 1388 1407
Size: 961 22 226 179 19
Fragments arranged by size:
961 226 179 22 19
Bsp1286I G_dGCh'C
Cuts at: 0 58 961 983 1209 1335 1388 1407
Size: 58 903 22 226 126 53 19
Fragments arranged by size:
903 226 126 58 53 22 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 117 1029 1407
Size: 117 912 378
Fragments arranged by size:
912 378 117
BsrBI CCG'CTC
Cuts at: 0 443 756 1407
Size: 443 313 651
Fragments arranged by size:
651 443 313
BsrFI r'CCGG_y
Cuts at: 0 316 1407
Size: 316 1091
BssSI C'ACGA_G
Cuts at: 0 427 1407
Size: 427 980
BstAPI GCAn_nnn'nTGC
Cuts at: 0 638 1011 1407
Size: 638 373 396
Fragments arranged by size:
638 396 373
BstYI r'GATC_y
Cuts at: 0 147 267 1046 1166 1407
Size: 147 120 779 120 241
Fragments arranged by size:
779 241 147 120 120
BtgI C'CryG_G
Cuts at: 0 308 1220 1238 1407
Size: 308 912 18 169
Fragments arranged by size:
912 308 169 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 372 1089 1407
Size: 372 717 318
Fragments arranged by size:
717 372 318
BtsI GCAGTG_nn'
Cuts at: 0 47 1407
Size: 47 1360
DraIII CAC_nnn'GTG
Cuts at: 0 860 1407
Size: 860 547
EaeI y'GGCC_r
Cuts at: 0 20 314 1025 1325 1340 1407
Size: 20 294 711 300 15 67
Fragments arranged by size:
711 300 294 67 20 15
EagI C'GGCC_G
Cuts at: 0 1325 1340 1407
Size: 1325 15 67
Fragments arranged by size:
1325 67 15
EarI CTCTTCn'nnn_
Cuts at: 0 530 688 1407
Size: 530 158 719
Fragments arranged by size:
719 530 158
EcoICRI GAG'CTC
Cuts at: 0 981 1407
Size: 981 426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 926 1147 1252 1407
Size: 253 673 221 105 155
Fragments arranged by size:
673 253 221 155 105
EcoO109I rG'GnC_Cy
Cuts at: 0 303 1008 1407
Size: 303 705 399
Fragments arranged by size:
705 399 303
HaeII r_GCGC'y
Cuts at: 0 664 1309 1407
Size: 664 645 98
Fragments arranged by size:
664 645 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 119 151 451 483 1407
Size: 119 32 300 32 924
Fragments arranged by size:
924 300 119 32 32
KasI G'GCGC_C
Cuts at: 0 1305 1407
Size: 1305 102
KpnI G_GTAC'C
Cuts at: 0 822 1407
Size: 822 585
MfeI C'AATT_G
Cuts at: 0 138 1407
Size: 138 1269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 638 734 836 1407
Size: 182 456 96 102 571
Fragments arranged by size:
571 456 182 102 96
MscI TGG'CCA
Cuts at: 0 1027 1407
Size: 1027 380
MslI CAynn'nnrTG
Cuts at: 0 391 394 1407
Size: 391 3 1013
Fragments arranged by size:
1013 391 3
MspA1I CmG'CkG
Cuts at: 0 473 747 876 1212 1240 1302 1407
Size: 473 274 129 336 28 62 105
Fragments arranged by size:
473 336 274 129 105 62 28
NaeI GCC'GGC
Cuts at: 0 318 1407
Size: 318 1089
NarI GG'CG_CC
Cuts at: 0 1306 1407
Size: 1306 101
NcoI C'CATG_G
Cuts at: 0 308 1407
Size: 308 1099
NgoMIV G'CCGG_C
Cuts at: 0 316 1407
Size: 316 1091
NspI r_CATG'y
Cuts at: 0 621 1407
Size: 621 786
PflMI CCAn_nnn'nTGG
Cuts at: 0 888 1176 1407
Size: 888 288 231
Fragments arranged by size:
888 288 231
PmlI CAC'GTG
Cuts at: 0 1176 1407
Size: 1176 231
PpuMI rG'GwC_Cy
Cuts at: 0 303 1407
Size: 303 1104
PspXI vC'TCGA_Gb
Cuts at: 0 1381 1407
Size: 1381 26
PstI C_TGCA'G
Cuts at: 0 1202 1355 1407
Size: 1202 153 52
Fragments arranged by size:
1202 153 52
PvuII CAG'CTG
Cuts at: 0 473 747 1212 1302 1407
Size: 473 274 465 90 105
Fragments arranged by size:
473 465 274 105 90
SacI G_AGCT'C
Cuts at: 0 983 1407
Size: 983 424
SacII CC_GC'GG
Cuts at: 0 1241 1407
Size: 1241 166
SfcI C'TryA_G
Cuts at: 0 1198 1351 1363 1407
Size: 1198 153 12 44
Fragments arranged by size:
1198 153 44 12
SfoI GGC'GCC
Cuts at: 0 1307 1407
Size: 1307 100
SmlI C'TyrA_G
Cuts at: 0 1381 1407
Size: 1381 26
StyI C'CwwG_G
Cuts at: 0 308 1407
Size: 308 1099
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 80 1407
Size: 80 1327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 951 983 1407
Size: 951 32 424
Fragments arranged by size:
951 424 32
XhoI C'TCGA_G
Cuts at: 0 1381 1407
Size: 1381 26
Enzymes that do cut:
AarI Acc65I AcuI AhdI AleI AlfI AlwNI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BssSI BstAPI BstYI BtgI BtgZI
BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I
HaeII Hin4I KasI KpnI MfeI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PflMI PmlI
PpuMI PspXI PstI PvuII SacI SacII SfcI SfoI
SmlI StyI TaqII TstI XhoI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AloI
ApaI AscI AseI AsiSI AvrII BaeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmtI BpuEI BsaI
BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI MluI NdeI NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XcmI XmaI XmnI ZraI