(Linear) (Six-base) MAPSORT of: KIEE1119.seq  Check: 8082  from: 1  to: 984



KIEE1119.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     446     823     984 
   Size:        349      97     377     161

  Fragments arranged by size: 

                 377     349     161      97
 
AflII C'TTAA_G

Cuts at:      0     799     984 
   Size:        799     185
 
ApoI r'AATT_y

Cuts at:      0     271     528     619     808     979     984 
   Size:        271     257      91     189     171       5

  Fragments arranged by size: 

                 271     257     189     171      91       5
 
AseI AT'TA_AT

Cuts at:      0      34     984 
   Size:         34     950
 
BanI G'GyrC_C

Cuts at:      0     595     984 
   Size:        595     389
 
BclI T'GATC_A

Cuts at:      0     686     984 
   Size:        686     298
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     410     471     984 
   Size:        410      61     513

  Fragments arranged by size: 

                 513     410      61
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     502     984 
   Size:        502     482
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     290     984 
   Size:        290     694
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     475     505     984 
   Size:        475      30     479

  Fragments arranged by size: 

                 479     475      30
 
Bsu36I CC'TnA_GG

Cuts at:      0     248     777     984 
   Size:        248     529     207

  Fragments arranged by size: 

                 529     248     207
 
BtsI GCAGTG_nn'

Cuts at:      0     194     984 
   Size:        194     790
 
DraI TTT'AAA

Cuts at:      0     527     984 
   Size:        527     457
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     446     502     823     984 
   Size:        349      97      56     321     161

  Fragments arranged by size: 

                 349     321     161      97      56
 
EcoO109I rG'GnC_Cy

Cuts at:      0     733     984 
   Size:        733     251
 
EcoRI G'AATT_C

Cuts at:      0     619     984 
   Size:        619     365
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     354     753     984 
   Size:        354     399     231

  Fragments arranged by size: 

                 399     354     231
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     112     144     760     792     984 
   Size:        112      32     616      32     192

  Fragments arranged by size: 

                 616     192     112      32      32
 
HincII GTy'rAC

Cuts at:      0     887     984 
   Size:        887      97
 
HindIII A'AGCT_T

Cuts at:      0     559     984 
   Size:        559     425
 
MfeI C'AATT_G

Cuts at:      0      50     984 
   Size:         50     934
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     626     984 
   Size:        626     358
 
MslI CAynn'nnrTG

Cuts at:      0     909     984 
   Size:        909      75
 
MspA1I CmG'CkG

Cuts at:      0     507     984 
   Size:        507     477
 
NspI r_CATG'y

Cuts at:      0     137     793     914     945     984 
   Size:        137     656     121      31      39

  Fragments arranged by size: 

                 656     137     121      39      31
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      64     984 
   Size:         64     920
 
PpuMI rG'GwC_Cy

Cuts at:      0     733     984 
   Size:        733     251
 
PvuII CAG'CTG

Cuts at:      0     507     984 
   Size:        507     477
 
SanDI GG'GwC_CC

Cuts at:      0     733     984 
   Size:        733     251
 
SfcI C'TryA_G

Cuts at:      0     567     825     984 
   Size:        567     258     159

  Fragments arranged by size: 

                 567     258     159
 
SmlI C'TyrA_G

Cuts at:      0     305     799     984 
   Size:        305     494     185

  Fragments arranged by size: 

                 494     305     185
 
SphI G_CATG'C

Cuts at:      0     793     984 
   Size:        793     191
 
SspI AAT'ATT

Cuts at:      0     876     984 
   Size:        876     108
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     475     507     984 
   Size:        475      32     477

  Fragments arranged by size: 

                 477     475      32
 

 Enzymes that do cut:

     AcuI    AflII     ApoI     AseI     BanI     BclI     BmrI     BpmI
    BpuEI    BsaXI   Bsu36I     BtsI     DraI  Eco57MI EcoO109I    EcoRI
     FalI    Hin4I   HincII  HindIII     MfeI     MmeI     MslI   MspA1I
     NspI    PflMI    PpuMI    PvuII    SanDI     SfcI     SmlI     SphI
     SspI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI