Restriction map for
pFN21AE1118
(Linear) (Six-base) MAPSORT of: KIEE1118.seq Check: 9832 from: 1 to: 981
KIEE1118.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 644 981
Size: 644 337
AflIII A'CryG_T
Cuts at: 0 811 981
Size: 811 170
AhdI GACnn_n'nnGTC
Cuts at: 0 417 981
Size: 417 564
AleI CACnn'nnGTG
Cuts at: 0 739 981
Size: 739 242
AlwNI CAG_nnn'CTG
Cuts at: 0 73 555 981
Size: 73 482 426
Fragments arranged by size:
482 426 73
ApaLI G'TGCA_C
Cuts at: 0 615 981
Size: 615 366
ApoI r'AATT_y
Cuts at: 0 598 981
Size: 598 383
BamHI G'GATC_C
Cuts at: 0 470 981
Size: 470 511
BanII G_rGCy'C
Cuts at: 0 236 981
Size: 236 745
BbsI GAAGACnn'nnnn_
Cuts at: 0 416 932 981
Size: 416 516 49
Fragments arranged by size:
516 416 49
BbvCI CC'TCA_GC
Cuts at: 0 907 981
Size: 907 74
BclI T'GATC_A
Cuts at: 0 917 981
Size: 917 64
BglI GCCn_nnn'nGGC
Cuts at: 0 968 981
Size: 968 13
BlpI GC'TnA_GC
Cuts at: 0 958 981
Size: 958 23
Bme1580I G_kGCm'C
Cuts at: 0 619 981
Size: 619 362
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 484 739 981
Size: 448 36 255 242
Fragments arranged by size:
448 255 242 36
Bpu10I CC'TnA_GC
Cuts at: 0 324 907 981
Size: 324 583 74
Fragments arranged by size:
583 324 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 76 211 697 981
Size: 76 135 486 284
Fragments arranged by size:
486 284 135 76
BsaHI Gr'CG_yC
Cuts at: 0 311 981
Size: 311 670
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 521 551 981
Size: 521 30 430
Fragments arranged by size:
521 430 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 445 736 981
Size: 445 291 245
Fragments arranged by size:
445 291 245
BsiHKAI G_wGCw'C
Cuts at: 0 143 236 619 981
Size: 143 93 383 362
Fragments arranged by size:
383 362 143 93
Bsp1286I G_dGCh'C
Cuts at: 0 143 236 619 981
Size: 143 93 383 362
Fragments arranged by size:
383 362 143 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 644 981
Size: 644 337
BstYI r'GATC_y
Cuts at: 0 353 470 552 981
Size: 353 117 82 429
Fragments arranged by size:
429 353 117 82
BtgI C'CryG_G
Cuts at: 0 708 981
Size: 708 273
BtsI GCAGTG_nn'
Cuts at: 0 630 981
Size: 630 351
ClaI AT'CG_AT
Cuts at: 0 134 981
Size: 134 847
DrdI GACnn_nn'nnGTC
Cuts at: 0 225 981
Size: 225 756
EarI CTCTTCn'nnn_
Cuts at: 0 40 647 981
Size: 40 607 334
Fragments arranged by size:
607 334 40
EcoICRI GAG'CTC
Cuts at: 0 234 981
Size: 234 747
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 484 739 981
Size: 448 36 255 242
Fragments arranged by size:
448 255 242 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 430 487 981
Size: 430 57 494
Fragments arranged by size:
494 430 57
EcoO109I rG'GnC_Cy
Cuts at: 0 207 821 981
Size: 207 614 160
Fragments arranged by size:
614 207 160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 403 435 521 553 981
Size: 403 32 86 32 428
Fragments arranged by size:
428 403 86 32 32
MluI A'CGCG_T
Cuts at: 0 811 981
Size: 811 170
MslI CAynn'nnrTG
Cuts at: 0 136 739 919 981
Size: 136 603 180 62
Fragments arranged by size:
603 180 136 62
MspA1I CmG'CkG
Cuts at: 0 165 381 935 981
Size: 165 216 554 46
Fragments arranged by size:
554 216 165 46
NcoI C'CATG_G
Cuts at: 0 708 981
Size: 708 273
PfoI T'CCnGG_A
Cuts at: 0 16 981
Size: 16 965
PpuMI rG'GwC_Cy
Cuts at: 0 207 821 981
Size: 207 614 160
Fragments arranged by size:
614 207 160
PstI C_TGCA'G
Cuts at: 0 491 981
Size: 491 490
PvuII CAG'CTG
Cuts at: 0 381 981
Size: 381 600
SacI G_AGCT'C
Cuts at: 0 236 981
Size: 236 745
SanDI GG'GwC_CC
Cuts at: 0 821 981
Size: 821 160
SapI GCTCTTCn'nnn_
Cuts at: 0 40 981
Size: 40 941
SbfI CC_TGCA'GG
Cuts at: 0 491 981
Size: 491 490
SfcI C'TryA_G
Cuts at: 0 487 981
Size: 487 494
StyI C'CwwG_G
Cuts at: 0 296 332 708 981
Size: 296 36 376 273
Fragments arranged by size:
376 296 273 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 766 981
Size: 766 215
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 735 981
Size: 735 246
Enzymes that do cut:
AarI AflIII AhdI AleI AlwNI ApaLI ApoI BamHI
BanII BbsI BbvCI BclI BglI BlpI Bme1580I BpmI
Bpu10I BsaI BsaHI BsaXI BseRI BsiHKAI Bsp1286I BspMI
BstYI BtgI BtsI ClaI DrdI EarI EcoICRI Eco57MI
EcoNI EcoO109I Hin4I MluI MslI MspA1I NcoI PfoI
PpuMI PstI PvuII SacI SanDI SapI SbfI SfcI
StyI TaqII XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BcgI BciVI BfrBI BglII BmgBI
BmrI BmtI BplI BpuEI BsaAI BsaBI BsaWI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI CspCI DraI DraIII EaeI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI