(Linear) (Six-base) MAPSORT of: KIEE1115.seq  Check: 2362  from: 1  to: 570



KIEE1115.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:33  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     191     237     570 
   Size:        191      46     333

  Fragments arranged by size: 

                 333     191      46
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     222     570 
   Size:        222     348
 
BanII G_rGCy'C

Cuts at:      0     286     404     570 
   Size:        286     118     166

  Fragments arranged by size: 

                 286     166     118
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     153     157     570 
   Size:        153       4     413

  Fragments arranged by size: 

                 413     153       4
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      47     392     570 
   Size:         47     345     178

  Fragments arranged by size: 

                 345     178      47
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550     570 
   Size:        550      20
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     178     570 
   Size:        178     392
 
BsaHI Gr'CG_yC

Cuts at:      0     158     570 
   Size:        158     412
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     320     570 
   Size:        320     250
 
BsiEI CG_ry'CG

Cuts at:      0      87     570 
   Size:         87     483
 
Bsp1286I G_dGCh'C

Cuts at:      0     286     404     570 
   Size:        286     118     166

  Fragments arranged by size: 

                 286     166     118
 
BsrBI CCG'CTC

Cuts at:      0     134     570 
   Size:        134     436
 
BsrDI GCAATG_nn'

Cuts at:      0      38     570 
   Size:         38     532
 
BsrGI T'GTAC_A

Cuts at:      0     482     570 
   Size:        482      88
 
BstEII G'GTnAC_C

Cuts at:      0     261     570 
   Size:        261     309
 
BtgI C'CryG_G

Cuts at:      0     113     570 
   Size:        113     457
 
DraI TTT'AAA

Cuts at:      0      63     459     570 
   Size:         63     396     111

  Fragments arranged by size: 

                 396     111      63
 
EaeI y'GGCC_r

Cuts at:      0     311     323     570 
   Size:        311      12     247

  Fragments arranged by size: 

                 311     247      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550     570 
   Size:        550      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     415     570 
   Size:        415     155
 
HaeII r_GCGC'y

Cuts at:      0     132     570 
   Size:        132     438
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     177     209     223     255     570 
   Size:        177      32      14      32     315

  Fragments arranged by size: 

                 315     177      32      32      14
 
MscI TGG'CCA

Cuts at:      0     325     570 
   Size:        325     245
 
MslI CAynn'nnrTG

Cuts at:      0     194     570 
   Size:        194     376
 
PfoI T'CCnGG_A

Cuts at:      0     145     570 
   Size:        145     425
 
PshAI GACnn'nnGTC

Cuts at:      0     157     570 
   Size:        157     413
 
PvuI CG_AT'CG

Cuts at:      0      87     570 
   Size:         87     483
 
SexAI A'CCwGG_T

Cuts at:      0     170     257     265     570 
   Size:        170      87       8     305

  Fragments arranged by size: 

                 305     170      87       8
 
SspI AAT'ATT

Cuts at:      0     463     570 
   Size:        463     107
 
StyI C'CwwG_G

Cuts at:      0     508     570 
   Size:        508      62
 
TatI w'GTAC_w

Cuts at:      0     482     570 
   Size:        482      88
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     506     570 
   Size:        506      64
 

 Enzymes that do cut:

     AhdI     AlfI    BanII     BbsI    BciVI     BpmI     BsaI    BsaHI
     BsgI    BsiEI Bsp1286I    BsrBI    BsrDI    BsrGI   BstEII     BtgI
     DraI     EaeI  Eco57MI    EcoNI    HaeII    Hin4I     MscI     MslI
     PfoI    PshAI     PvuI    SexAI     SspI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrFI   BssHII    BssSI   BstAPI
    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI