(Linear) (Six-base) MAPSORT of: KIEE1114.seq  Check: 7819  from: 1  to: 480



KIEE1114.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     448     480 
   Size:        448      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     267     480 
   Size:        267     213
 
BamHI G'GATC_C

Cuts at:      0     278     480 
   Size:        278     202
 
BanI G'GyrC_C

Cuts at:      0       5     480 
   Size:          5     475
 
BanII G_rGCy'C

Cuts at:      0      81     232     247     480 
   Size:         81     151      15     233

  Fragments arranged by size: 

                 233     151      81      15
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     119     308     480 
   Size:        119     189     172

  Fragments arranged by size: 

                 189     172     119
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     226     324     480 
   Size:        226      98     156

  Fragments arranged by size: 

                 226     156      98
 
Bpu10I CC'TnA_GC

Cuts at:      0      80     480 
   Size:         80     400
 
BsaHI Gr'CG_yC

Cuts at:      0     407     480 
   Size:        407      73
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      81     480 
   Size:         81     399
 
BseYI C'CCAG_C

Cuts at:      0      90     480 
   Size:         90     390
 
Bsp1286I G_dGCh'C

Cuts at:      0      81     232     247     480 
   Size:         81     151      15     233

  Fragments arranged by size: 

                 233     151      81      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     448     480 
   Size:        448      32
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     362     480 
   Size:        362     118
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      16     480 
   Size:         16     464
 
BstYI r'GATC_y

Cuts at:      0     278     325     480 
   Size:        278      47     155

  Fragments arranged by size: 

                 278     155      47
 
BtgI C'CryG_G

Cuts at:      0      15     480 
   Size:         15     465
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      26     480 
   Size:         26     454
 
EaeI y'GGCC_r

Cuts at:      0      38     480 
   Size:         38     442
 
EarI CTCTTCn'nnn_

Cuts at:      0     458     480 
   Size:        458      22
 
MslI CAynn'nnrTG

Cuts at:      0      14     480 
   Size:         14     466
 
MspA1I CmG'CkG

Cuts at:      0     267     321     480 
   Size:        267      54     159

  Fragments arranged by size: 

                 267     159      54
 
NcoI C'CATG_G

Cuts at:      0      15     480 
   Size:         15     465
 
PvuII CAG'CTG

Cuts at:      0     267     321     480 
   Size:        267      54     159

  Fragments arranged by size: 

                 267     159      54
 
SapI GCTCTTCn'nnn_

Cuts at:      0     458     480 
   Size:        458      22
 
StyI C'CwwG_G

Cuts at:      0      15     480 
   Size:         15     465
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     204     236     480 
   Size:        204      32     244

  Fragments arranged by size: 

                 244     204      32
 

 Enzymes that do cut:

     AarI    AlwNI    BamHI     BanI    BanII    BciVI     BmrI   Bpu10I
    BsaHI    BseRI    BseYI Bsp1286I    BspMI   BstAPI    BstXI    BstYI
     BtgI     DrdI     EaeI     EarI     MslI   MspA1I     NcoI    PvuII
     SapI     StyI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI