Restriction map for
pFN21AE1113
(Linear) (Six-base) MAPSORT of: KIEE1113.seq Check: 8319 from: 1 to: 462
KIEE1113.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 443 462
Size: 443 19
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 178 462
Size: 178 284
AlwNI CAG_nnn'CTG
Cuts at: 0 258 462
Size: 258 204
ApaI G_GGCC'C
Cuts at: 0 295 462
Size: 295 167
AvaI C'yCGr_G
Cuts at: 0 37 212 462
Size: 37 175 250
Fragments arranged by size:
250 175 37
BanI G'GyrC_C
Cuts at: 0 394 462
Size: 394 68
BanII G_rGCy'C
Cuts at: 0 295 462
Size: 295 167
BbvCI CC'TCA_GC
Cuts at: 0 146 462
Size: 146 316
BclI T'GATC_A
Cuts at: 0 219 462
Size: 219 243
Bme1580I G_kGCm'C
Cuts at: 0 295 399 462
Size: 295 104 63
Fragments arranged by size:
295 104 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 182 462
Size: 182 280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 124 462
Size: 115 9 338
Fragments arranged by size:
338 115 9
Bpu10I CC'TnA_GC
Cuts at: 0 146 462
Size: 146 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 462
Size: 297 165
BsaI GGTCTCn'nnnn_
Cuts at: 0 430 462
Size: 430 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 427 457 462
Size: 427 30 5
Fragments arranged by size:
427 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 462
Size: 280 182
BseYI C'CCAG_C
Cuts at: 0 335 462
Size: 335 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 462
Size: 390 72
Bsp1286I G_dGCh'C
Cuts at: 0 295 399 462
Size: 295 104 63
Fragments arranged by size:
295 104 63
BsrFI r'CCGG_y
Cuts at: 0 86 462
Size: 86 376
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 311 462
Size: 311 151
DraI TTT'AAA
Cuts at: 0 228 462
Size: 228 234
EaeI y'GGCC_r
Cuts at: 0 284 462
Size: 284 178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 124 462
Size: 115 9 338
Fragments arranged by size:
338 115 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 262 462
Size: 262 200
EcoO109I rG'GnC_Cy
Cuts at: 0 33 99 255 291 292 319 425 462
Size: 33 66 156 36 1 27 106 37
Fragments arranged by size:
156 106 66 37 36 33 27 1
HaeII r_GCGC'y
Cuts at: 0 342 462
Size: 342 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 429 461 462
Size: 429 32 1
Fragments arranged by size:
429 32 1
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 462
Size: 242 220
MspA1I CmG'CkG
Cuts at: 0 45 93 389 462
Size: 45 48 296 73
Fragments arranged by size:
296 73 48 45
PfoI T'CCnGG_A
Cuts at: 0 119 462
Size: 119 343
PpuMI rG'GwC_Cy
Cuts at: 0 33 319 425 462
Size: 33 286 106 37
Fragments arranged by size:
286 106 37 33
PspOMI G'GGCC_C
Cuts at: 0 291 462
Size: 291 171
PvuII CAG'CTG
Cuts at: 0 93 389 462
Size: 93 296 73
Fragments arranged by size:
296 93 73
SanDI GG'GwC_CC
Cuts at: 0 319 425 462
Size: 319 106 37
Fragments arranged by size:
319 106 37
SexAI A'CCwGG_T
Cuts at: 0 439 462
Size: 439 23
SmaI CCC'GGG
Cuts at: 0 39 462
Size: 39 423
SmlI C'TyrA_G
Cuts at: 0 276 462
Size: 276 186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 427 459 462
Size: 427 32 3
Fragments arranged by size:
427 32 3
XmaI C'CCGG_G
Cuts at: 0 37 462
Size: 37 425
Enzymes that do cut:
AhdI AlfI AlwNI ApaI AvaI BanI BanII BbvCI
BclI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaXI
BseRI BseYI BsgI Bsp1286I BsrFI BtgZI DraI EaeI
Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MspA1I PfoI
PpuMI PspOMI PvuII SanDI SexAI SmaI SmlI TstI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BsaAI
BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtsI ClaI CspCI DraIII DrdI EagI EarI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI Tth111I XbaI XcmI
XhoI XmnI ZraI