(Linear) (Six-base) MAPSORT of: KIEE1110.seq  Check: 8712  from: 1  to: 348



KIEE1110.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     233     341     348 
   Size:        140      93     108       7

  Fragments arranged by size: 

                 140     108      93       7
 
AvaI C'yCGr_G

Cuts at:      0      82     348 
   Size:         82     266
 
BamHI G'GATC_C

Cuts at:      0     150     348 
   Size:        150     198
 
BglI GCCn_nnn'nGGC

Cuts at:      0      78     348 
   Size:         78     270
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     257     348 
   Size:        257      91
 
Bpu10I CC'TnA_GC

Cuts at:      0     164     348 
   Size:        164     184
 
BsaAI yAC'GTr

Cuts at:      0      48     348 
   Size:         48     300
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     319     348 
   Size:        319      29
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     184     348 
   Size:        184     164
 
BstEII G'GTnAC_C

Cuts at:      0     235     348 
   Size:        235     113
 
BstYI r'GATC_y

Cuts at:      0     150     348 
   Size:        150     198
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     233     341     348 
   Size:        140      93     108       7

  Fragments arranged by size: 

                 140     108      93       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     122     323     348 
   Size:        122     201      25

  Fragments arranged by size: 

                 201     122      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     172     204     348 
   Size:        172      32     144

  Fragments arranged by size: 

                 172     144      32
 
MspA1I CmG'CkG

Cuts at:      0     160     348 
   Size:        160     188
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     247     348 
   Size:        247     101
 
PstI C_TGCA'G

Cuts at:      0     159     195     348 
   Size:        159      36     153

  Fragments arranged by size: 

                 159     153      36
 
PvuII CAG'CTG

Cuts at:      0     160     348 
   Size:        160     188
 
SfcI C'TryA_G

Cuts at:      0     155     191     348 
   Size:        155      36     157

  Fragments arranged by size: 

                 157     155      36
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      78     348 
   Size:         78     270
 
SnaBI TAC'GTA

Cuts at:      0      48     348 
   Size:         48     300
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     239     254     348 
   Size:        239      15      94

  Fragments arranged by size: 

                 239      94      15
 

 Enzymes that do cut:

     AcuI     AvaI    BamHI     BglI     BmrI   Bpu10I    BsaAI    BseRI
    BspMI   BstEII    BstYI  Eco57MI    EcoNI    Hin4I   MspA1I    PflMI
     PstI    PvuII     SfcI     SfiI    SnaBI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI