(Linear) (Six-base) MAPSORT of: KIEE1108.seq  Check: 7397  from: 1  to: 309



KIEE1108.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     309 
   Size:         49     260
 
ApoI r'AATT_y

Cuts at:      0     274     309 
   Size:        274      35
 
AvaI C'yCGr_G

Cuts at:      0     233     309 
   Size:        233      76
 
BglI GCCn_nnn'nGGC

Cuts at:      0      67      76     309 
   Size:         67       9     233

  Fragments arranged by size: 

                 233      67       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     116     309 
   Size:        116     193
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     309 
   Size:        191     118
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      91     309 
   Size:         91     218
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      48     280     283     309 
   Size:         48     232       3      26

  Fragments arranged by size: 

                 232      48      26       3
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     135     309 
   Size:        135     174
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     171     309 
   Size:        171     138
 
EarI CTCTTCn'nnn_

Cuts at:      0      25     309 
   Size:         25     284
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     191     309 
   Size:         49     142     118

  Fragments arranged by size: 

                 142     118      49
 
EcoRI G'AATT_C

Cuts at:      0     274     309 
   Size:        274      35
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     309 
   Size:         77     232
 
MslI CAynn'nnrTG

Cuts at:      0     169     309 
   Size:        169     140
 
SfcI C'TryA_G

Cuts at:      0     180     309 
   Size:        180     129
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      67      76     309 
   Size:         67       9     233

  Fragments arranged by size: 

                 233      67       9
 
SpeI A'CTAG_T

Cuts at:      0     168     309 
   Size:        168     141
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI     BglI     BmrI     BpmI     BsaI    BseRI
    BspMI    BstXI     EarI  Eco57MI    EcoRI     MmeI     MslI     SfcI
     SfiI     SpeI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI