(Linear) (Six-base) MAPSORT of: KIEE1107.seq  Check: 3586  from: 1  to: 291



KIEE1107.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:33  ..

ApoI r'AATT_y

Cuts at:      0     256     291 
   Size:        256      35
 
AvaI C'yCGr_G

Cuts at:      0     215     291 
   Size:        215      76
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      98     291 
   Size:         98     193
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     291 
   Size:        173     118
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      73     291 
   Size:         73     218
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      48     262     265     291 
   Size:         48     214       3      26

  Fragments arranged by size: 

                 214      48      26       3
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     117     291 
   Size:        117     174
 
BsrFI r'CCGG_y

Cuts at:      0      19     291 
   Size:         19     272
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     153     291 
   Size:        153     138
 
DraI TTT'AAA

Cuts at:      0     177     291 
   Size:        177     114
 
EarI CTCTTCn'nnn_

Cuts at:      0      25     291 
   Size:         25     266
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     291 
   Size:        173     118
 
EcoRI G'AATT_C

Cuts at:      0     256     291 
   Size:        256      35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27     291 
   Size:         27     264
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     291 
   Size:         77     214
 
MslI CAynn'nnrTG

Cuts at:      0     151     291 
   Size:        151     140
 
NaeI GCC'GGC

Cuts at:      0      21     291 
   Size:         21     270
 
NgoMIV G'CCGG_C

Cuts at:      0      19     291 
   Size:         19     272
 
SfcI C'TryA_G

Cuts at:      0     162     291 
   Size:        162     129
 
SpeI A'CTAG_T

Cuts at:      0     150     291 
   Size:        150     141
 

 Enzymes that do cut:

     ApoI     AvaI     BmrI     BpmI     BsaI    BseRI    BspMI    BsrFI
    BstXI     DraI     EarI  Eco57MI    EcoRI    Hin4I     MmeI     MslI
     NaeI   NgoMIV     SfcI     SpeI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI