(Linear) (Six-base) MAPSORT of: KIEE1106.seq  Check: 8818  from: 1  to: 285



KIEE1106.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:33  ..

BanI G'GyrC_C

Cuts at:      0     280     285 
   Size:        280       5
 
BanII G_rGCy'C

Cuts at:      0     134     285 
   Size:        134     151
 
Bme1580I G_kGCm'C

Cuts at:      0      18     285 
   Size:         18     267
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     285 
   Size:        181     104
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     142     285 
   Size:        142     143
 
BseYI C'CCAG_C

Cuts at:      0     264     285 
   Size:        264      21
 
BsiHKAI G_wGCw'C

Cuts at:      0     134     285 
   Size:        134     151
 
Bsp1286I G_dGCh'C

Cuts at:      0      18     134     285 
   Size:         18     116     151

  Fragments arranged by size: 

                 151     116      18
 
BsrDI GCAATG_nn'

Cuts at:      0     191     285 
   Size:        191      94
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     225     285 
   Size:        225      60
 
EaeI y'GGCC_r

Cuts at:      0      59     285 
   Size:         59     226
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     250     285 
   Size:        250      35
 
EcoICRI GAG'CTC

Cuts at:      0     132     285 
   Size:        132     153
 
EcoO109I rG'GnC_Cy

Cuts at:      0     119     143     285 
   Size:        119      24     142

  Fragments arranged by size: 

                 142     119      24
 
HincII GTy'rAC

Cuts at:      0     165     285 
   Size:        165     120
 
MscI TGG'CCA

Cuts at:      0      61     285 
   Size:         61     224
 
PpuMI rG'GwC_Cy

Cuts at:      0     119     143     285 
   Size:        119      24     142

  Fragments arranged by size: 

                 142     119      24
 
PstI C_TGCA'G

Cuts at:      0     208     221     285 
   Size:        208      13      64

  Fragments arranged by size: 

                 208      64      13
 
SacI G_AGCT'C

Cuts at:      0     134     285 
   Size:        134     151
 
SanDI GG'GwC_CC

Cuts at:      0     119     285 
   Size:        119     166
 
SfcI C'TryA_G

Cuts at:      0     204     217     285 
   Size:        204      13      68

  Fragments arranged by size: 

                 204      68      13
 
SmlI C'TyrA_G

Cuts at:      0     196     285 
   Size:        196      89
 
StyI C'CwwG_G

Cuts at:      0     146     176     285 
   Size:        146      30     109

  Fragments arranged by size: 

                 146     109      30
 
TatI w'GTAC_w

Cuts at:      0     155     285 
   Size:        155     130
 

 Enzymes that do cut:

     BanI    BanII Bme1580I    BpuEI    BseRI    BseYI  BsiHKAI Bsp1286I
    BsrDI   BstAPI     EaeI     EciI  EcoICRI EcoO109I   HincII     MscI
    PpuMI     PstI     SacI    SanDI     SfcI     SmlI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI  Eco57MI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI