Restriction map for
pFN21AE1104
(Linear) (Six-base) MAPSORT of: KIEE1104.seq Check: 2481 from: 1 to: 483
KIEE1104.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
Acc65I G'GTAC_C
Cuts at: 0 275 483
Size: 275 208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 223 349 483
Size: 223 126 134
Fragments arranged by size:
223 134 126
AlwNI CAG_nnn'CTG
Cuts at: 0 336 483
Size: 336 147
ApaI G_GGCC'C
Cuts at: 0 55 483
Size: 55 428
BanI G'GyrC_C
Cuts at: 0 275 483
Size: 275 208
BanII G_rGCy'C
Cuts at: 0 55 185 483
Size: 55 130 298
Fragments arranged by size:
298 130 55
BbvCI CC'TCA_GC
Cuts at: 0 271 425 483
Size: 271 154 58
Fragments arranged by size:
271 154 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 133 167 483
Size: 133 34 316
Fragments arranged by size:
316 133 34
BglI GCCn_nnn'nGGC
Cuts at: 0 211 242 483
Size: 211 31 241
Fragments arranged by size:
241 211 31
Bme1580I G_kGCm'C
Cuts at: 0 55 207 483
Size: 55 152 276
Fragments arranged by size:
276 152 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 199 211 301 385 483
Size: 121 78 12 90 84 98
Fragments arranged by size:
121 98 90 84 78 12
Bpu10I CC'TnA_GC
Cuts at: 0 232 271 295 425 483
Size: 232 39 24 130 58
Fragments arranged by size:
232 130 58 39 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 483
Size: 208 275
BsaI GGTCTCn'nnnn_
Cuts at: 0 318 483
Size: 318 165
BsaHI Gr'CG_yC
Cuts at: 0 128 483
Size: 128 355
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 298 328 483
Size: 298 30 155
Fragments arranged by size:
298 155 30
BseYI C'CCAG_C
Cuts at: 0 48 483
Size: 48 435
BsiHKAI G_wGCw'C
Cuts at: 0 301 483
Size: 301 182
Bsp1286I G_dGCh'C
Cuts at: 0 55 185 207 301 483
Size: 55 130 22 94 182
Fragments arranged by size:
182 130 94 55 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 276 483
Size: 276 207
Bsu36I CC'TnA_GG
Cuts at: 0 419 483
Size: 419 64
BtgI C'CryG_G
Cuts at: 0 79 483
Size: 79 404
BtsI GCAGTG_nn'
Cuts at: 0 207 483
Size: 207 276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 199 211 301 385 483
Size: 121 78 12 90 84 98
Fragments arranged by size:
121 98 90 84 78 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 283 476 483
Size: 283 193 7
Fragments arranged by size:
283 193 7
EcoO109I rG'GnC_Cy
Cuts at: 0 228 288 483
Size: 228 60 195
Fragments arranged by size:
228 195 60
HincII GTy'rAC
Cuts at: 0 306 483
Size: 306 177
KpnI G_GTAC'C
Cuts at: 0 279 483
Size: 279 204
MslI CAynn'nnrTG
Cuts at: 0 331 483
Size: 331 152
MspA1I CmG'CkG
Cuts at: 0 141 483
Size: 141 342
PasI CC'CwG_GG
Cuts at: 0 456 483
Size: 456 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 336 483
Size: 336 147
PpuMI rG'GwC_Cy
Cuts at: 0 288 483
Size: 288 195
PspOMI G'GGCC_C
Cuts at: 0 51 483
Size: 51 432
PstI C_TGCA'G
Cuts at: 0 89 146 483
Size: 89 57 337
Fragments arranged by size:
337 89 57
PvuII CAG'CTG
Cuts at: 0 141 483
Size: 141 342
SfcI C'TryA_G
Cuts at: 0 85 142 483
Size: 85 57 341
Fragments arranged by size:
341 85 57
SmlI C'TyrA_G
Cuts at: 0 223 483
Size: 223 260
Enzymes that do cut:
Acc65I AlfI AlwNI ApaI BanI BanII BbvCI BcgI
BglI Bme1580I BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseYI BsiHKAI Bsp1286I BspMI Bsu36I BtgI BtsI Eco57MI
EcoNI EcoO109I HincII KpnI MslI MspA1I PasI PflMI
PpuMI PspOMI PstI PvuII SfcI SmlI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
BsaAI BsaBI BsaWI BseRI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HindIII HpaI KasI MfeI MluI MmeI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI