Restriction map for
pFN21AE1103
(Linear) (Six-base) MAPSORT of: KIEE1103.seq Check: 2227 from: 1 to: 411
KIEE1103.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 151 343 411
Size: 73 78 192 68
Fragments arranged by size:
192 78 73 68
AvaI C'yCGr_G
Cuts at: 0 140 411
Size: 140 271
BanI G'GyrC_C
Cuts at: 0 307 411
Size: 307 104
BanII G_rGCy'C
Cuts at: 0 394 411
Size: 394 17
BbeI G_GCGC'C
Cuts at: 0 311 411
Size: 311 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 200 411
Size: 200 211
BbvCI CC'TCA_GC
Cuts at: 0 106 411
Size: 106 305
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 311 411
Size: 311 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 411
Size: 306 105
Bpu10I CC'TnA_GC
Cuts at: 0 106 388 411
Size: 106 282 23
Fragments arranged by size:
282 106 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 411
Size: 49 362
BsaHI Gr'CG_yC
Cuts at: 0 308 411
Size: 308 103
BsaWI w'CCGG_w
Cuts at: 0 44 411
Size: 44 367
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 411
Size: 115 296
BseYI C'CCAG_C
Cuts at: 0 300 411
Size: 300 111
BsiHKAI G_wGCw'C
Cuts at: 0 86 394 411
Size: 86 308 17
Fragments arranged by size:
308 86 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 232 411
Size: 232 179
Bsp1286I G_dGCh'C
Cuts at: 0 86 394 411
Size: 86 308 17
Fragments arranged by size:
308 86 17
BsrFI r'CCGG_y
Cuts at: 0 9 270 411
Size: 9 261 141
Fragments arranged by size:
261 141 9
BssSI C'ACGA_G
Cuts at: 0 85 411
Size: 85 326
BtsI GCAGTG_nn'
Cuts at: 0 215 411
Size: 215 196
EarI CTCTTCn'nnn_
Cuts at: 0 204 280 411
Size: 204 76 131
Fragments arranged by size:
204 131 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 411
Size: 28 383
EcoICRI GAG'CTC
Cuts at: 0 392 411
Size: 392 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 151 306 343 411
Size: 73 78 155 37 68
Fragments arranged by size:
155 78 73 68 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 344 411
Size: 344 67
EcoO109I rG'GnC_Cy
Cuts at: 0 372 411
Size: 372 39
HaeII r_GCGC'y
Cuts at: 0 311 411
Size: 311 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 411
Size: 181 32 198
Fragments arranged by size:
198 181 32
KasI G'GCGC_C
Cuts at: 0 307 411
Size: 307 104
MspA1I CmG'CkG
Cuts at: 0 300 411
Size: 300 111
NaeI GCC'GGC
Cuts at: 0 11 411
Size: 11 400
NarI GG'CG_CC
Cuts at: 0 308 411
Size: 308 103
NgoMIV G'CCGG_C
Cuts at: 0 9 411
Size: 9 402
PfoI T'CCnGG_A
Cuts at: 0 95 411
Size: 95 316
PpuMI rG'GwC_Cy
Cuts at: 0 372 411
Size: 372 39
PstI C_TGCA'G
Cuts at: 0 176 411
Size: 176 235
SacI G_AGCT'C
Cuts at: 0 394 411
Size: 394 17
SanDI GG'GwC_CC
Cuts at: 0 372 411
Size: 372 39
SfcI C'TryA_G
Cuts at: 0 172 411
Size: 172 239
SfoI GGC'GCC
Cuts at: 0 309 411
Size: 309 102
SmaI CCC'GGG
Cuts at: 0 142 411
Size: 142 269
SmlI C'TyrA_G
Cuts at: 0 64 411
Size: 64 347
StuI AGG'CCT
Cuts at: 0 364 411
Size: 364 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 179 267 411
Size: 179 88 144
Fragments arranged by size:
179 144 88
XmaI C'CCGG_G
Cuts at: 0 140 411
Size: 140 271
Enzymes that do cut:
AcuI AvaI BanI BanII BbeI BbsI BbvCI BplI
BpmI Bpu10I BpuEI BsaHI BsaWI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BsrFI BssSI BtsI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MspA1I NaeI
NarI NgoMIV PfoI PpuMI PstI SacI SanDI SfcI
SfoI SmaI SmlI StuI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BsaI BsaAI BsaBI BsaXI BsgI BsiEI
BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StyI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI