Restriction map for
pFN21AE1100
(Linear) (Six-base) MAPSORT of: KIEE1100.seq Check: 8234 from: 1 to: 915
KIEE1100.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 915
Size: 113 802
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 625 657 915
Size: 625 32 258
Fragments arranged by size:
625 258 32
AlwNI CAG_nnn'CTG
Cuts at: 0 148 915
Size: 148 767
ApaLI G'TGCA_C
Cuts at: 0 678 915
Size: 678 237
ApoI r'AATT_y
Cuts at: 0 768 915
Size: 768 147
BamHI G'GATC_C
Cuts at: 0 9 915
Size: 9 906
BbsI GAAGACnn'nnnn_
Cuts at: 0 113 269 295 424 914 915
Size: 113 156 26 129 490 1
Fragments arranged by size:
490 156 129 113 26 1
BbvCI CC'TCA_GC
Cuts at: 0 358 400 507 915
Size: 358 42 107 408
Fragments arranged by size:
408 358 107 42
Bme1580I G_kGCm'C
Cuts at: 0 682 915
Size: 682 233
BmgBI CAC'GTC
Cuts at: 0 912 915
Size: 912 3
BmrI ACTGGGnnnn_n'
Cuts at: 0 578 915
Size: 578 337
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 168 915
Size: 168 747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 636 666 915
Size: 349 287 30 249
Fragments arranged by size:
349 287 249 30
Bpu10I CC'TnA_GC
Cuts at: 0 358 400 507 915
Size: 358 42 107 408
Fragments arranged by size:
408 358 107 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 138 168 915
Size: 138 30 747
Fragments arranged by size:
747 138 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 146 915
Size: 146 769
BseYI C'CCAG_C
Cuts at: 0 314 523 808 915
Size: 314 209 285 107
Fragments arranged by size:
314 285 209 107
BsiHKAI G_wGCw'C
Cuts at: 0 682 915
Size: 682 233
Bsp1286I G_dGCh'C
Cuts at: 0 682 915
Size: 682 233
BsrDI GCAATG_nn'
Cuts at: 0 492 915
Size: 492 423
BstXI CCAn_nnnn'nTGG
Cuts at: 0 786 915
Size: 786 129
BstYI r'GATC_y
Cuts at: 0 9 174 915
Size: 9 165 741
Fragments arranged by size:
741 165 9
BtgI C'CryG_G
Cuts at: 0 170 866 915
Size: 170 696 49
Fragments arranged by size:
696 170 49
EaeI y'GGCC_r
Cuts at: 0 472 915
Size: 472 443
EarI CTCTTCn'nnn_
Cuts at: 0 164 312 540 915
Size: 164 148 228 375
Fragments arranged by size:
375 228 164 148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 349 636 666 915
Size: 113 236 287 30 249
Fragments arranged by size:
287 249 236 113 30
EcoO109I rG'GnC_Cy
Cuts at: 0 270 915
Size: 270 645
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 50 915
Size: 50 865
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 138 170 915
Size: 26 112 32 745
Fragments arranged by size:
745 112 32 26
HindIII A'AGCT_T
Cuts at: 0 27 915
Size: 27 888
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 410 915
Size: 74 336 505
Fragments arranged by size:
505 336 74
MslI CAynn'nnrTG
Cuts at: 0 895 915
Size: 895 20
MspA1I CmG'CkG
Cuts at: 0 318 915
Size: 318 597
NcoI C'CATG_G
Cuts at: 0 170 866 915
Size: 170 696 49
Fragments arranged by size:
696 170 49
NspI r_CATG'y
Cuts at: 0 756 900 915
Size: 756 144 15
Fragments arranged by size:
756 144 15
PfoI T'CCnGG_A
Cuts at: 0 643 658 688 915
Size: 643 15 30 227
Fragments arranged by size:
643 227 30 15
PpuMI rG'GwC_Cy
Cuts at: 0 270 915
Size: 270 645
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 388 420 915
Size: 388 32 495
Fragments arranged by size:
495 388 32
PstI C_TGCA'G
Cuts at: 0 240 746 915
Size: 240 506 169
Fragments arranged by size:
506 240 169
PvuII CAG'CTG
Cuts at: 0 318 915
Size: 318 597
ScaI AGT'ACT
Cuts at: 0 855 915
Size: 855 60
SexAI A'CCwGG_T
Cuts at: 0 421 673 915
Size: 421 252 242
Fragments arranged by size:
421 252 242
SfcI C'TryA_G
Cuts at: 0 236 742 915
Size: 236 506 173
Fragments arranged by size:
506 236 173
SphI G_CATG'C
Cuts at: 0 756 915
Size: 756 159
StuI AGG'CCT
Cuts at: 0 505 703 818 915
Size: 505 198 115 97
Fragments arranged by size:
505 198 115 97
StyI C'CwwG_G
Cuts at: 0 170 797 866 915
Size: 170 627 69 49
Fragments arranged by size:
627 170 69 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 328 697 915
Size: 328 369 218
Fragments arranged by size:
369 328 218
TatI w'GTAC_w
Cuts at: 0 324 853 915
Size: 324 529 62
Fragments arranged by size:
529 324 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 542 574 915
Size: 542 32 341
Fragments arranged by size:
542 341 32
Tth111I GACn'n_nGTC
Cuts at: 0 268 915
Size: 268 647
Enzymes that do cut:
AcuI AloI AlwNI ApaLI ApoI BamHI BbsI BbvCI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaXI BseRI
BseYI BsiHKAI Bsp1286I BsrDI BstXI BstYI BtgI EaeI
EarI Eco57MI EcoO109I FalI Hin4I HindIII MmeI MslI
MspA1I NcoI NspI PfoI PpuMI PsrI PstI PvuII
ScaI SexAI SfcI SphI StuI StyI TaqII TatI
TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BanI BanII BbeI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmtI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI SwaI XbaI XcmI XhoI XmaI
XmnI ZraI