Restriction map for
pFN21AE1087
(Linear) (Six-base) MAPSORT of: KIEE1087.seq Check: 2558 from: 1 to: 3717
KIEE1087.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 326 3717
Size: 326 3391
Acc65I G'GTAC_C
Cuts at: 0 1715 1909 3717
Size: 1715 194 1808
Fragments arranged by size:
1808 1715 194
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1675 3717
Size: 1675 2042
AfeI AGC'GCT
Cuts at: 0 2890 3650 3717
Size: 2890 760 67
Fragments arranged by size:
2890 760 67
AflIII A'CryG_T
Cuts at: 0 1196 3717
Size: 1196 2521
AgeI A'CCGG_T
Cuts at: 0 3557 3717
Size: 3557 160
AleI CACnn'nnGTG
Cuts at: 0 2656 2812 3717
Size: 2656 156 905
Fragments arranged by size:
2656 905 156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1202 2897 3717
Size: 1202 1695 820
Fragments arranged by size:
1695 1202 820
AlwNI CAG_nnn'CTG
Cuts at: 0 441 681 846 1173 1653 2001 3072 3717
Size: 441 240 165 327 480 348 1071 645
Fragments arranged by size:
1071 645 480 441 348 327 240 165
ApaI G_GGCC'C
Cuts at: 0 582 1560 1765 2366 3717
Size: 582 978 205 601 1351
Fragments arranged by size:
1351 978 601 582 205
ApaLI G'TGCA_C
Cuts at: 0 1075 3717
Size: 1075 2642
ApoI r'AATT_y
Cuts at: 0 1480 3717
Size: 1480 2237
AvaI C'yCGr_G
Cuts at: 0 237 538 568 1118 1758 3717
Size: 237 301 30 550 640 1959
Fragments arranged by size:
1959 640 550 301 237 30
BanI G'GyrC_C
Cuts at: 0 31 754 1138 1715 1869 1909 2329 2353
Size: 31 723 384 577 154 40 420 24
Cuts at: 2353 2480 2542 3560 3717
Size: 127 62 1018 157
Fragments arranged by size:
1018 723 577 420 384 157 154 127
62 40 31 24
BanII G_rGCy'C
Cuts at: 0 335 582 828 1364 1560 1673 1765 2366
Size: 335 247 246 536 196 113 92 601
Cuts at: 2366 2570 3246 3254 3310 3717
Size: 204 676 8 56 407
Fragments arranged by size:
676 601 536 407 335 247 246 204
196 113 92 56 8
BbeI G_GCGC'C
Cuts at: 0 35 3717
Size: 35 3682
BbsI GAAGACnn'nnnn_
Cuts at: 0 1448 1589 3717
Size: 1448 141 2128
Fragments arranged by size:
2128 1448 141
BbvCI CC'TCA_GC
Cuts at: 0 167 1774 2102 3717
Size: 167 1607 328 1615
Fragments arranged by size:
1615 1607 328 167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 228 262 3717
Size: 228 34 3455
Fragments arranged by size:
3455 228 34
BclI T'GATC_A
Cuts at: 0 2474 3717
Size: 2474 1243
BfrBI ATG'CAT
Cuts at: 0 2730 3717
Size: 2730 987
BglI GCCn_nnn'nGGC
Cuts at: 0 1683 1878 2023 2574 3717
Size: 1683 195 145 551 1143
Fragments arranged by size:
1683 1143 551 195 145
BlpI GC'TnA_GC
Cuts at: 0 910 2885 3717
Size: 910 1975 832
Fragments arranged by size:
1975 910 832
Bme1580I G_kGCm'C
Cuts at: 0 356 582 806 1079 1141 1191 1351 1560
Size: 356 226 224 273 62 50 160 209
Cuts at: 1560 1765 2366 2785 3157 3717
Size: 205 601 419 372 560
Fragments arranged by size:
601 560 419 372 356 273 226 224
209 205 160 62 50
BmgBI CAC'GTC
Cuts at: 0 366 594 1197 2223 3717
Size: 366 228 603 1026 1494
Fragments arranged by size:
1494 1026 603 366 228
BmrI ACTGGGnnnn_n'
Cuts at: 0 1255 1329 3025 3516 3620 3717
Size: 1255 74 1696 491 104 97
Fragments arranged by size:
1696 1255 491 104 97 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 714 3717
Size: 714 3003
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 63 472 760 1303 1348 1483 1939
Size: 36 27 409 288 543 45 135 456
Cuts at: 1939 2421 3268 3717
Size: 482 847 449
Fragments arranged by size:
847 543 482 456 449 409 288 135
45 36 27
Bpu10I CC'TnA_GC
Cuts at: 0 167 1774 2102 3717
Size: 167 1607 328 1615
Fragments arranged by size:
1615 1607 328 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3202 3717
Size: 3202 515
BsaI GGTCTCn'nnnn_
Cuts at: 0 81 3717
Size: 81 3636
BsaAI yAC'GTr
Cuts at: 0 3211 3717
Size: 3211 506
BsaHI Gr'CG_yC
Cuts at: 0 32 2711 3717
Size: 32 2679 1006
Fragments arranged by size:
2679 1006 32
BsaWI w'CCGG_w
Cuts at: 0 1718 3557 3717
Size: 1718 1839 160
Fragments arranged by size:
1839 1718 160
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1597 1627 3717
Size: 1597 30 2090
Fragments arranged by size:
2090 1597 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 586 1372 1618 1621 1624 1762 2044 3442
Size: 586 786 246 3 3 138 282 1398
Cuts at: 3442 3717
Size: 275
Fragments arranged by size:
1398 786 586 282 275 246 138 3
3
BseYI C'CCAG_C
Cuts at: 0 71 103 479 1017 1552 1688 1765 1856
Size: 71 32 376 538 535 136 77 91
Cuts at: 1856 1927 2183 2188 2869 2945 3222 3501 3717
Size: 71 256 5 681 76 277 279 216
Fragments arranged by size:
681 538 535 376 279 277 256 216
136 91 77 76 71 71 32 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 690 1819 2653 2721 3563 3717
Size: 232 458 1129 834 68 842 154
Fragments arranged by size:
1129 842 834 458 232 154 68
BsiEI CG_ry'CG
Cuts at: 0 2856 3717
Size: 2856 861
BsiHKAI G_wGCw'C
Cuts at: 0 317 828 1079 1364 1673 2373 3310 3717
Size: 317 511 251 285 309 700 937 407
Fragments arranged by size:
937 700 511 407 317 309 285 251
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2702 3717
Size: 2702 1015
Bsp1286I G_dGCh'C
Cuts at: 0 317 335 356 582 806 828 1079 1141
Size: 317 18 21 226 224 22 251 62
Cuts at: 1141 1191 1351 1364 1560 1673 1765 2366 2373
Size: 50 160 13 196 113 92 601 7
Cuts at: 2373 2570 2785 3157 3246 3254 3310 3717
Size: 197 215 372 89 8 56 407
Fragments arranged by size:
601 407 372 317 251 226 224 215
197 196 160 113 92 89 62 56
50 22 21 18 13 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 326 1310 1481 3717
Size: 326 984 171 2236
Fragments arranged by size:
2236 984 326 171
BsrBI CCG'CTC
Cuts at: 0 576 1236 3717
Size: 576 660 2481
Fragments arranged by size:
2481 660 576
BsrDI GCAATG_nn'
Cuts at: 0 2211 2595 3188 3717
Size: 2211 384 593 529
Fragments arranged by size:
2211 593 529 384
BsrFI r'CCGG_y
Cuts at: 0 28 51 720 1273 2017 2539 2722 2912
Size: 28 23 669 553 744 522 183 190
Cuts at: 2912 3557 3717
Size: 645 160
Fragments arranged by size:
744 669 645 553 522 190 183 160
28 23
BsrGI T'GTAC_A
Cuts at: 0 1964 3717
Size: 1964 1753
BssSI C'ACGA_G
Cuts at: 0 250 3717
Size: 250 3467
BstAPI GCAn_nnn'nTGC
Cuts at: 0 441 2004 3072 3717
Size: 441 1563 1068 645
Fragments arranged by size:
1563 1068 645 441
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2206 2814 3717
Size: 2206 608 903
Fragments arranged by size:
2206 903 608
BstYI r'GATC_y
Cuts at: 0 1392 3069 3717
Size: 1392 1677 648
Fragments arranged by size:
1677 1392 648
Bsu36I CC'TnA_GG
Cuts at: 0 3565 3717
Size: 3565 152
BtgI C'CryG_G
Cuts at: 0 293 557 1571 2163 2199 2627 3581 3717
Size: 293 264 1014 592 36 428 954 136
Fragments arranged by size:
1014 954 592 428 293 264 136 36
BtsI GCAGTG_nn'
Cuts at: 0 1059 1605 2138 2619 2758 3717
Size: 1059 546 533 481 139 959
Fragments arranged by size:
1059 959 546 533 481 139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2668 2703 3717
Size: 2668 35 1014
Fragments arranged by size:
2668 1014 35
DraIII CAC_nnn'GTG
Cuts at: 0 1263 3717
Size: 1263 2454
DrdI GACnn_nn'nnGTC
Cuts at: 0 598 3717
Size: 598 3119
EaeI y'GGCC_r
Cuts at: 0 26 458 560 638 896 1271 1397 1892
Size: 26 432 102 78 258 375 126 495
Cuts at: 1892 2853 3341 3717
Size: 961 488 376
Fragments arranged by size:
961 495 488 432 376 375 258 126
102 78 26
EagI C'GGCC_G
Cuts at: 0 2853 3717
Size: 2853 864
EarI CTCTTCn'nnn_
Cuts at: 0 683 1005 1243 2767 3263 3356 3717
Size: 683 322 238 1524 496 93 361
Fragments arranged by size:
1524 683 496 361 322 238 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 406 529 2041 3717
Size: 406 123 1512 1676
Fragments arranged by size:
1676 1512 406 123
EcoICRI GAG'CTC
Cuts at: 0 826 1362 1671 3308 3717
Size: 826 536 309 1637 409
Fragments arranged by size:
1637 826 536 409 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 63 472 760 1303 1348 1483 1675
Size: 36 27 409 288 543 45 135 192
Cuts at: 1675 1939 2421 3268 3717
Size: 264 482 847 449
Fragments arranged by size:
847 543 482 449 409 288 264 192
135 45 36 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 850 1336 2026 3568 3717
Size: 850 486 690 1542 149
Fragments arranged by size:
1542 850 690 486 149
EcoO109I rG'GnC_Cy
Cuts at: 0 579 744 1096 1170 1458 1470 1556 1557
Size: 579 165 352 74 288 12 86 1
Cuts at: 1557 1762 2363 2637 2970 3717
Size: 205 601 274 333 747
Fragments arranged by size:
747 601 579 352 333 288 274 205
165 86 74 12 1
FseI GG_CCGG'CC
Cuts at: 0 2021 3717
Size: 2021 1696
FspI TGC'GCA
Cuts at: 0 3076 3717
Size: 3076 641
HaeII r_GCGC'y
Cuts at: 0 35 72 2892 3384 3652 3717
Size: 35 37 2820 492 268 65
Fragments arranged by size:
2820 492 268 65 37 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 815 847 2659 2691 3717
Size: 815 32 1812 32 1026
Fragments arranged by size:
1812 1026 815 32 32
HincII GTy'rAC
Cuts at: 0 1059 1311 2143 3318 3717
Size: 1059 252 832 1175 399
Fragments arranged by size:
1175 1059 832 399 252
HindIII A'AGCT_T
Cuts at: 0 3178 3717
Size: 3178 539
KasI G'GCGC_C
Cuts at: 0 31 3717
Size: 31 3686
KpnI G_GTAC'C
Cuts at: 0 1719 1913 3717
Size: 1719 194 1804
Fragments arranged by size:
1804 1719 194
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 2972 3626 3717
Size: 122 2850 654 91
Fragments arranged by size:
2850 654 122 91
MscI TGG'CCA
Cuts at: 0 562 640 898 1399 1894 3343 3717
Size: 562 78 258 501 495 1449 374
Fragments arranged by size:
1449 562 501 495 374 258 78
MslI CAynn'nnrTG
Cuts at: 0 229 886 892 2204 2264 2656 2812 3337
Size: 229 657 6 1312 60 392 156 525
Cuts at: 3337 3717
Size: 380
Fragments arranged by size:
1312 657 525 392 380 229 156 60
6
MspA1I CmG'CkG
Cuts at: 0 117 204 295 483 678 714 1368 1416
Size: 117 87 91 188 195 36 654 48
Cuts at: 1416 1927 2001 2698 2935 2978 3717
Size: 511 74 697 237 43 739
Fragments arranged by size:
739 697 654 511 237 195 188 117
91 87 74 48 43 36
NaeI GCC'GGC
Cuts at: 0 30 722 1275 2019 2541 2724 3717
Size: 30 692 553 744 522 183 993
Fragments arranged by size:
993 744 692 553 522 183 30
NarI GG'CG_CC
Cuts at: 0 32 3717
Size: 32 3685
NcoI C'CATG_G
Cuts at: 0 1571 2199 2627 3581 3717
Size: 1571 628 428 954 136
Fragments arranged by size:
1571 954 628 428 136
NdeI CA'TA_TG
Cuts at: 0 2059 3717
Size: 2059 1658
NgoMIV G'CCGG_C
Cuts at: 0 28 720 1273 2017 2539 2722 3717
Size: 28 692 553 744 522 183 995
Fragments arranged by size:
995 744 692 553 522 183 28
NsiI A_TGCA'T
Cuts at: 0 2732 3717
Size: 2732 985
NspI r_CATG'y
Cuts at: 0 867 891 1281 2730 2836 3489 3717
Size: 867 24 390 1449 106 653 228
Fragments arranged by size:
1449 867 653 390 228 106 24
PasI CC'CwG_GG
Cuts at: 0 3639 3717
Size: 3639 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 681 2436 3350 3717
Size: 681 1755 914 367
Fragments arranged by size:
1755 914 681 367
PfoI T'CCnGG_A
Cuts at: 0 872 1166 1698 3263 3717
Size: 872 294 532 1565 454
Fragments arranged by size:
1565 872 532 454 294
PpuMI rG'GwC_Cy
Cuts at: 0 744 1170 1458 1470 3717
Size: 744 426 288 12 2247
Fragments arranged by size:
2247 744 426 288 12
PspOMI G'GGCC_C
Cuts at: 0 578 1556 1761 2362 3717
Size: 578 978 205 601 1355
Fragments arranged by size:
1355 978 601 578 205
PstI C_TGCA'G
Cuts at: 0 323 1600 1784 2133 2339 2765 3068 3717
Size: 323 1277 184 349 206 426 303 649
Fragments arranged by size:
1277 649 426 349 323 303 206 184
PvuII CAG'CTG
Cuts at: 0 204 483 714 1416 1927 2001 2698 2935
Size: 204 279 231 702 511 74 697 237
Cuts at: 2935 3717
Size: 782
Fragments arranged by size:
782 702 697 511 279 237 231 204
74
SacI G_AGCT'C
Cuts at: 0 828 1364 1673 3310 3717
Size: 828 536 309 1637 407
Fragments arranged by size:
1637 828 536 407 309
SacII CC_GC'GG
Cuts at: 0 296 3717
Size: 296 3421
SapI GCTCTTCn'nnn_
Cuts at: 0 683 1243 2767 3717
Size: 683 560 1524 950
Fragments arranged by size:
1524 950 683 560
SbfI CC_TGCA'GG
Cuts at: 0 2339 3068 3717
Size: 2339 729 649
Fragments arranged by size:
2339 729 649
ScaI AGT'ACT
Cuts at: 0 1183 3717
Size: 1183 2534
SexAI A'CCwGG_T
Cuts at: 0 3416 3478 3717
Size: 3416 62 239
Fragments arranged by size:
3416 239 62
SfcI C'TryA_G
Cuts at: 0 319 1596 1780 1944 2129 2335 2761 3064
Size: 319 1277 184 164 185 206 426 303
Cuts at: 3064 3717
Size: 653
Fragments arranged by size:
1277 653 426 319 303 206 185 184
164
SfoI GGC'GCC
Cuts at: 0 33 3717
Size: 33 3684
SmaI CCC'GGG
Cuts at: 0 1760 3717
Size: 1760 1957
SmlI C'TyrA_G
Cuts at: 0 237 1118 3181 3717
Size: 237 881 2063 536
Fragments arranged by size:
2063 881 536 237
SphI G_CATG'C
Cuts at: 0 867 891 1281 2730 2836 3717
Size: 867 24 390 1449 106 881
Fragments arranged by size:
1449 881 867 390 106 24
StuI AGG'CCT
Cuts at: 0 2504 3717
Size: 2504 1213
StyI C'CwwG_G
Cuts at: 0 1571 2174 2199 2300 2627 2966 3581 3689
Size: 1571 603 25 101 327 339 615 108
Cuts at: 3689 3717
Size: 28
Fragments arranged by size:
1571 615 603 339 327 108 101 28
25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 784 836 1222 1274 1279 2197 2618 2650
Size: 784 52 386 52 5 918 421 32
Cuts at: 2650 2738 3285 3717
Size: 88 547 432
Fragments arranged by size:
918 784 547 432 421 386 88 52
52 32 5
TatI w'GTAC_w
Cuts at: 0 1181 1964 3717
Size: 1181 783 1753
Fragments arranged by size:
1753 1181 783
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 796 828 1450 1482 3033 3065 3717
Size: 796 32 622 32 1551 32 652
Fragments arranged by size:
1551 796 652 622 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 633 1696 3687 3717
Size: 633 1063 1991 30
Fragments arranged by size:
1991 1063 633 30
XhoI C'TCGA_G
Cuts at: 0 237 1118 3717
Size: 237 881 2599
Fragments arranged by size:
2599 881 237
XmaI C'CCGG_G
Cuts at: 0 1758 3717
Size: 1758 1959
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PflMI PfoI PpuMI
PspOMI PstI PvuII SacI SacII SapI SbfI ScaI
SexAI SfcI SfoI SmaI SmlI SphI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AhdI AloI AscI AseI
AsiSI AvrII BaeI BamHI BciVI BglII BmtI BsaBI
BsiWI BsmI BspEI BspHI BssHII BstBI BstEII BstZ17I
BtgZI ClaI DraI EcoRI EcoRV FalI FspAI HpaI
MfeI MluI NheI NotI NruI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SfiI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XmnI ZraI