Restriction map for
pFN21AE1017
(Linear) (Six-base) MAPSORT of: KIEE1017.seq Check: 8898 from: 1 to: 1419
KIEE1017.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 971 974 1200 1285 1419
Size: 413 558 3 226 85 134
Fragments arranged by size:
558 413 226 134 85 3
AfeI AGC'GCT
Cuts at: 0 1255 1419
Size: 1255 164
AhdI GACnn_n'nnGTC
Cuts at: 0 678 1419
Size: 678 741
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 958 990 1419
Size: 958 32 429
Fragments arranged by size:
958 429 32
ApaLI G'TGCA_C
Cuts at: 0 800 1419
Size: 800 619
ApoI r'AATT_y
Cuts at: 0 407 440 623 1419
Size: 407 33 183 796
Fragments arranged by size:
796 407 183 33
BanII G_rGCy'C
Cuts at: 0 183 583 1308 1419
Size: 183 400 725 111
Fragments arranged by size:
725 400 183 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 1297 1419
Size: 1297 122
BbvCI CC'TCA_GC
Cuts at: 0 704 1419
Size: 704 715
BglI GCCn_nnn'nGGC
Cuts at: 0 990 1419
Size: 990 429
Bme1580I G_kGCm'C
Cuts at: 0 804 1419
Size: 804 615
BmrI ACTGGGnnnn_n'
Cuts at: 0 529 616 1419
Size: 529 87 803
Fragments arranged by size:
803 529 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 929 962 1322 1419
Size: 283 646 33 360 97
Fragments arranged by size:
646 360 283 97 33
Bpu10I CC'TnA_GC
Cuts at: 0 704 1419
Size: 704 715
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1419
Size: 829 590
BsaHI Gr'CG_yC
Cuts at: 0 318 1419
Size: 318 1101
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 756 786 958 988 1419
Size: 756 30 172 30 431
Fragments arranged by size:
756 431 172 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 658 661 664 692 895 926 1419
Size: 658 3 3 28 203 31 493
Fragments arranged by size:
658 493 203 31 28 3 3
BseYI C'CCAG_C
Cuts at: 0 51 1391 1419
Size: 51 1340 28
Fragments arranged by size:
1340 51 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 812 1419
Size: 181 631 607
Fragments arranged by size:
631 607 181
BsiHKAI G_wGCw'C
Cuts at: 0 403 804 1286 1419
Size: 403 401 482 133
Fragments arranged by size:
482 403 401 133
BsmI GAATG_Cn'
Cuts at: 0 509 1419
Size: 509 910
BsmBI CGTCTCn'nnnn_
Cuts at: 0 256 325 1419
Size: 256 69 1094
Fragments arranged by size:
1094 256 69
Bsp1286I G_dGCh'C
Cuts at: 0 183 403 583 804 1286 1308 1419
Size: 183 220 180 221 482 22 111
Fragments arranged by size:
482 221 220 183 180 111 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 52 375 1419
Size: 52 323 1044
Fragments arranged by size:
1044 323 52
BsrFI r'CCGG_y
Cuts at: 0 1196 1419
Size: 1196 223
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1206 1419
Size: 1206 213
BstYI r'GATC_y
Cuts at: 0 1038 1419
Size: 1038 381
Bsu36I CC'TnA_GG
Cuts at: 0 875 1419
Size: 875 544
BtgI C'CryG_G
Cuts at: 0 58 450 1419
Size: 58 392 969
Fragments arranged by size:
969 392 58
BtsI GCAGTG_nn'
Cuts at: 0 197 754 829 1184 1397 1419
Size: 197 557 75 355 213 22
Fragments arranged by size:
557 355 213 197 75 22
EaeI y'GGCC_r
Cuts at: 0 362 1329 1419
Size: 362 967 90
Fragments arranged by size:
967 362 90
EarI CTCTTCn'nnn_
Cuts at: 0 347 522 635 1419
Size: 347 175 113 784
Fragments arranged by size:
784 347 175 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 313 1351 1419
Size: 313 1038 68
Fragments arranged by size:
1038 313 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 413 929 962 971 974 1200 1285
Size: 283 130 516 33 9 3 226 85
Cuts at: 1285 1322 1419
Size: 37 97
Fragments arranged by size:
516 283 226 130 97 85 37 33
9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 160 587 1378 1419
Size: 160 427 791 41
Fragments arranged by size:
791 427 160 41
EcoO109I rG'GnC_Cy
Cuts at: 0 1075 1371 1419
Size: 1075 296 48
Fragments arranged by size:
1075 296 48
EcoRV GAT'ATC
Cuts at: 0 887 1419
Size: 887 532
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 584 1419
Size: 584 835
HaeII r_GCGC'y
Cuts at: 0 1257 1419
Size: 1257 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 385 417 664 696 756 788 871 903
Size: 385 32 247 32 60 32 83 32
Cuts at: 903 1419
Size: 516
Fragments arranged by size:
516 385 247 83 60 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 781 1419
Size: 781 638
MscI TGG'CCA
Cuts at: 0 364 1419
Size: 364 1055
MslI CAynn'nnrTG
Cuts at: 0 1049 1419
Size: 1049 370
NspI r_CATG'y
Cuts at: 0 1315 1419
Size: 1315 104
PflMI CCAn_nnn'nTGG
Cuts at: 0 1006 1419
Size: 1006 413
PfoI T'CCnGG_A
Cuts at: 0 1024 1419
Size: 1024 395
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 121 153 958 990 1419
Size: 121 32 805 32 429
Fragments arranged by size:
805 429 121 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1075 1371 1419
Size: 1075 296 48
Fragments arranged by size:
1075 296 48
PstI C_TGCA'G
Cuts at: 0 1137 1419
Size: 1137 282
SapI GCTCTTCn'nnn_
Cuts at: 0 347 522 1419
Size: 347 175 897
Fragments arranged by size:
897 347 175
SfcI C'TryA_G
Cuts at: 0 494 1133 1419
Size: 494 639 286
Fragments arranged by size:
639 494 286
SmlI C'TyrA_G
Cuts at: 0 808 1419
Size: 808 611
SphI G_CATG'C
Cuts at: 0 1315 1419
Size: 1315 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 767 1419
Size: 767 652
TatI w'GTAC_w
Cuts at: 0 537 1419
Size: 537 882
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 50 82 1419
Size: 50 32 1337
Fragments arranged by size:
1337 50 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1099 1419
Size: 1099 320
Enzymes that do cut:
AcuI AfeI AhdI AloI ApaLI ApoI BanII BbsI
BbvCI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrFI BstAPI BstYI Bsu36I BtgI BtsI EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I HindIII MscI MslI NspI PflMI PfoI PpiI
PpuMI PstI SapI SfcI SmlI SphI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BsaI BsaAI
BsaBI BsaWI BsiEI BsiWI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI StyI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI