Restriction map for
pFN21AE1009
(Linear) (Six-base) MAPSORT of: KIEE1009.seq Check: 4535 from: 1 to: 660
KIEE1009.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 660
Size: 12 648
AleI CACnn'nnGTG
Cuts at: 0 343 660
Size: 343 317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 361 660
Size: 361 299
ApaI G_GGCC'C
Cuts at: 0 525 660
Size: 525 135
AvaI C'yCGr_G
Cuts at: 0 148 355 660
Size: 148 207 305
Fragments arranged by size:
305 207 148
BanII G_rGCy'C
Cuts at: 0 356 525 585 660
Size: 356 169 60 75
Fragments arranged by size:
356 169 75 60
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 660
Size: 56 604
BbvCI CC'TCA_GC
Cuts at: 0 403 660
Size: 403 257
BlpI GC'TnA_GC
Cuts at: 0 550 660
Size: 550 110
Bme1580I G_kGCm'C
Cuts at: 0 525 660
Size: 525 135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 307 508 660
Size: 256 51 201 152
Fragments arranged by size:
256 201 152 51
Bpu10I CC'TnA_GC
Cuts at: 0 403 660
Size: 403 257
BsaHI Gr'CG_yC
Cuts at: 0 421 660
Size: 421 239
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 286 452 660
Size: 97 189 166 208
Fragments arranged by size:
208 189 166 97
BseYI C'CCAG_C
Cuts at: 0 142 310 468 660
Size: 142 168 158 192
Fragments arranged by size:
192 168 158 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 660
Size: 586 74
BsiHKAI G_wGCw'C
Cuts at: 0 308 660
Size: 308 352
Bsp1286I G_dGCh'C
Cuts at: 0 308 356 525 585 660
Size: 308 48 169 60 75
Fragments arranged by size:
308 169 75 60 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 125 660
Size: 125 535
BsrBI CCG'CTC
Cuts at: 0 372 460 660
Size: 372 88 200
Fragments arranged by size:
372 200 88
BsrFI r'CCGG_y
Cuts at: 0 331 602 660
Size: 331 271 58
Fragments arranged by size:
331 271 58
BssHII G'CGCG_C
Cuts at: 0 448 660
Size: 448 212
BtgI C'CryG_G
Cuts at: 0 167 439 660
Size: 167 272 221
Fragments arranged by size:
272 221 167
EarI CTCTTCn'nnn_
Cuts at: 0 28 660
Size: 28 632
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 170 660
Size: 170 490
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 256 307 508 660
Size: 12 244 51 201 152
Fragments arranged by size:
244 201 152 51 12
EcoO109I rG'GnC_Cy
Cuts at: 0 247 291 359 660
Size: 247 44 68 301
Fragments arranged by size:
301 247 68 44
HaeII r_GCGC'y
Cuts at: 0 12 484 543 557 648 660
Size: 12 472 59 14 91 12
Fragments arranged by size:
472 91 59 14 12 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 660
Size: 343 32 285
Fragments arranged by size:
343 285 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 660
Size: 59 601
MslI CAynn'nnrTG
Cuts at: 0 343 660
Size: 343 317
MspA1I CmG'CkG
Cuts at: 0 169 441 519 531 570 660
Size: 169 272 78 12 39 90
Fragments arranged by size:
272 169 90 78 39 12
NspI r_CATG'y
Cuts at: 0 318 660
Size: 318 342
PfoI T'CCnGG_A
Cuts at: 0 374 660
Size: 374 286
PpuMI rG'GwC_Cy
Cuts at: 0 291 359 660
Size: 291 68 301
Fragments arranged by size:
301 291 68
PspOMI G'GGCC_C
Cuts at: 0 521 660
Size: 521 139
PstI C_TGCA'G
Cuts at: 0 518 660
Size: 518 142
SacII CC_GC'GG
Cuts at: 0 170 442 660
Size: 170 272 218
Fragments arranged by size:
272 218 170
SanDI GG'GwC_CC
Cuts at: 0 359 660
Size: 359 301
SfcI C'TryA_G
Cuts at: 0 514 660
Size: 514 146
SmaI CCC'GGG
Cuts at: 0 150 357 660
Size: 150 207 303
Fragments arranged by size:
303 207 150
StyI C'CwwG_G
Cuts at: 0 557 660
Size: 557 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 156 247 660
Size: 156 91 413
Fragments arranged by size:
413 156 91
Tth111I GACn'n_nGTC
Cuts at: 0 257 660
Size: 257 403
XmaI C'CCGG_G
Cuts at: 0 148 355 660
Size: 148 207 305
Fragments arranged by size:
305 207 148
Enzymes that do cut:
AcuI AleI AlfI ApaI AvaI BanII BbsI BbvCI
BlpI Bme1580I BpmI Bpu10I BsaHI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BtgI EarI
EciI Eco57MI EcoO109I HaeII Hin4I MmeI MslI MspA1I
NspI PfoI PpuMI PspOMI PstI SacII SanDI SfcI
SmaI StyI TaqII Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI XbaI XcmI XhoI XmnI ZraI