Restriction map for
pFN21AE1008
(Linear) (Six-base) MAPSORT of: KIEE1008.seq Check: 7721 from: 1 to: 612
KIEE1008.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
ApaI G_GGCC'C
Cuts at: 0 159 612
Size: 159 453
AvaI C'yCGr_G
Cuts at: 0 184 247 433 612
Size: 184 63 186 179
Fragments arranged by size:
186 184 179 63
BamHI G'GATC_C
Cuts at: 0 288 612
Size: 288 324
BanI G'GyrC_C
Cuts at: 0 6 162 300 612
Size: 6 156 138 312
Fragments arranged by size:
312 156 138 6
BanII G_rGCy'C
Cuts at: 0 103 159 230 248 275 612
Size: 103 56 71 18 27 337
Fragments arranged by size:
337 103 71 56 27 18
BbeI G_GCGC'C
Cuts at: 0 10 166 304 612
Size: 10 156 138 308
Fragments arranged by size:
308 156 138 10
BbvCI CC'TCA_GC
Cuts at: 0 442 612
Size: 442 170
BglI GCCn_nnn'nGGC
Cuts at: 0 154 207 234 279 612
Size: 154 53 27 45 333
Fragments arranged by size:
333 154 53 45 27
Bme1580I G_kGCm'C
Cuts at: 0 159 612
Size: 159 453
BmgBI CAC'GTC
Cuts at: 0 582 612
Size: 582 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 505 612
Size: 242 263 107
Fragments arranged by size:
263 242 107
Bpu10I CC'TnA_GC
Cuts at: 0 442 612
Size: 442 170
BsaHI Gr'CG_yC
Cuts at: 0 7 163 301 612
Size: 7 156 138 311
Fragments arranged by size:
311 156 138 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 229 331 361 409 412 493 502 505
Size: 229 102 30 48 3 81 9 3
Cuts at: 505 612
Size: 107
Fragments arranged by size:
229 107 102 81 48 30 9 3
3
BseYI C'CCAG_C
Cuts at: 0 237 612
Size: 237 375
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 612
Size: 604 8
BsiEI CG_ry'CG
Cuts at: 0 14 95 143 612
Size: 14 81 48 469
Fragments arranged by size:
469 81 48 14
BsiHKAI G_wGCw'C
Cuts at: 0 248 612
Size: 248 364
BsmBI CGTCTCn'nnnn_
Cuts at: 0 547 612
Size: 547 65
Bsp1286I G_dGCh'C
Cuts at: 0 103 159 230 248 275 612
Size: 103 56 71 18 27 337
Fragments arranged by size:
337 103 71 56 27 18
BspHI T'CATG_A
Cuts at: 0 536 612
Size: 536 76
BsrFI r'CCGG_y
Cuts at: 0 9 189 612
Size: 9 180 423
Fragments arranged by size:
423 180 9
BssHII G'CGCG_C
Cuts at: 0 116 612
Size: 116 496
BstXI CCAn_nnnn'nTGG
Cuts at: 0 51 612
Size: 51 561
BstYI r'GATC_y
Cuts at: 0 288 612
Size: 288 324
Bsu36I CC'TnA_GG
Cuts at: 0 392 612
Size: 392 220
BtgI C'CryG_G
Cuts at: 0 203 230 293 612
Size: 203 27 63 319
Fragments arranged by size:
319 203 63 27
EaeI y'GGCC_r
Cuts at: 0 11 23 41 92 140 612
Size: 11 12 18 51 48 472
Fragments arranged by size:
472 51 48 18 12 11
EagI C'GGCC_G
Cuts at: 0 11 92 140 612
Size: 11 81 48 472
Fragments arranged by size:
472 81 48 11
EarI CTCTTCn'nnn_
Cuts at: 0 338 612
Size: 338 274
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 328 612
Size: 328 284
EcoICRI GAG'CTC
Cuts at: 0 246 612
Size: 246 366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 505 612
Size: 242 263 107
Fragments arranged by size:
263 242 107
EcoO109I rG'GnC_Cy
Cuts at: 0 155 156 388 612
Size: 155 1 232 224
Fragments arranged by size:
232 224 155 1
HaeII r_GCGC'y
Cuts at: 0 10 166 304 612
Size: 10 156 138 308
Fragments arranged by size:
308 156 138 10
KasI G'GCGC_C
Cuts at: 0 6 162 300 612
Size: 6 156 138 312
Fragments arranged by size:
312 156 138 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 612
Size: 596 16
MscI TGG'CCA
Cuts at: 0 43 612
Size: 43 569
MspA1I CmG'CkG
Cuts at: 0 205 232 295 307 516 612
Size: 205 27 63 12 209 96
Fragments arranged by size:
209 205 96 63 27 12
NaeI GCC'GGC
Cuts at: 0 11 191 612
Size: 11 180 421
Fragments arranged by size:
421 180 11
NarI GG'CG_CC
Cuts at: 0 7 163 301 612
Size: 7 156 138 311
Fragments arranged by size:
311 156 138 7
NgoMIV G'CCGG_C
Cuts at: 0 9 189 612
Size: 9 180 423
Fragments arranged by size:
423 180 9
NotI GC'GGCC_GC
Cuts at: 0 92 612
Size: 92 520
PspOMI G'GGCC_C
Cuts at: 0 155 612
Size: 155 457
PvuII CAG'CTG
Cuts at: 0 516 612
Size: 516 96
SacI G_AGCT'C
Cuts at: 0 248 612
Size: 248 364
SacII CC_GC'GG
Cuts at: 0 206 233 296 612
Size: 206 27 63 316
Fragments arranged by size:
316 206 63 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 154 612
Size: 154 458
SfoI GGC'GCC
Cuts at: 0 8 164 302 612
Size: 8 156 138 310
Fragments arranged by size:
310 156 138 8
SmaI CCC'GGG
Cuts at: 0 435 612
Size: 435 177
TatI w'GTAC_w
Cuts at: 0 455 612
Size: 455 157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 480 612
Size: 480 132
XmaI C'CCGG_G
Cuts at: 0 433 612
Size: 433 179
Enzymes that do cut:
ApaI AvaI BamHI BanI BanII BbeI BbvCI BglI
Bme1580I BmgBI BpmI Bpu10I BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BsrFI BssHII BstXI
BstYI Bsu36I BtgI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I HaeII KasI MmeI MscI MspA1I NaeI
NarI NgoMIV NotI PspOMI PvuII SacI SacII SfiI
SfoI SmaI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbsI BcgI
BciVI BclI BfrBI BglII BlpI BmrI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI
BspEI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MslI
NcoI NdeI NheI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TstI Tth111I XbaI XhoI XmnI ZraI