Restriction map for
pFN21AE0999
(Linear) (Six-base) MAPSORT of: KIEE0999.seq Check: 2100 from: 1 to: 1569
KIEE0999.orf
With 182 enzymes: SgfI *
July 3, 2012 16:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 539 1569
Size: 539 1030
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 350 1189 1569
Size: 335 15 839 380
Fragments arranged by size:
839 380 335 15
AflIII A'CryG_T
Cuts at: 0 468 1569
Size: 468 1101
AlwNI CAG_nnn'CTG
Cuts at: 0 583 1569
Size: 583 986
ApaLI G'TGCA_C
Cuts at: 0 859 1569
Size: 859 710
ApoI r'AATT_y
Cuts at: 0 844 1569
Size: 844 725
AvaI C'yCGr_G
Cuts at: 0 102 116 1569
Size: 102 14 1453
Fragments arranged by size:
1453 102 14
BamHI G'GATC_C
Cuts at: 0 1534 1569
Size: 1534 35
BanI G'GyrC_C
Cuts at: 0 1484 1569
Size: 1484 85
BbsI GAAGACnn'nnnn_
Cuts at: 0 216 531 1569
Size: 216 315 1038
Fragments arranged by size:
1038 315 216
BbvCI CC'TCA_GC
Cuts at: 0 1418 1569
Size: 1418 151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 525 559 1569
Size: 525 34 1010
Fragments arranged by size:
1010 525 34
BclI T'GATC_A
Cuts at: 0 918 1569
Size: 918 651
BglII A'GATC_T
Cuts at: 0 768 1569
Size: 768 801
Bme1580I G_kGCm'C
Cuts at: 0 577 863 1569
Size: 577 286 706
Fragments arranged by size:
706 577 286
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 117 1569
Size: 117 1452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 1017 1301 1516 1569
Size: 700 317 284 215 53
Fragments arranged by size:
700 317 284 215 53
Bpu10I CC'TnA_GC
Cuts at: 0 1418 1569
Size: 1418 151
BsaI GGTCTCn'nnnn_
Cuts at: 0 1427 1569
Size: 1427 142
BsaAI yAC'GTr
Cuts at: 0 469 1569
Size: 469 1100
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 611 1569
Size: 611 958
BseYI C'CCAG_C
Cuts at: 0 420 1129 1569
Size: 420 709 440
Fragments arranged by size:
709 440 420
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1179 1419 1569
Size: 34 1145 240 150
Fragments arranged by size:
1145 240 150 34
BsiHKAI G_wGCw'C
Cuts at: 0 103 309 863 1569
Size: 103 206 554 706
Fragments arranged by size:
706 554 206 103
Bsp1286I G_dGCh'C
Cuts at: 0 103 309 577 863 1569
Size: 103 206 268 286 706
Fragments arranged by size:
706 286 268 206 103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 537 539 1569
Size: 537 2 1030
Fragments arranged by size:
1030 537 2
BsrDI GCAATG_nn'
Cuts at: 0 1346 1489 1569
Size: 1346 143 80
Fragments arranged by size:
1346 143 80
BsrFI r'CCGG_y
Cuts at: 0 76 1569
Size: 76 1493
BsrGI T'GTAC_A
Cuts at: 0 1286 1569
Size: 1286 283
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1491 1569
Size: 1491 78
BstYI r'GATC_y
Cuts at: 0 768 1197 1534 1569
Size: 768 429 337 35
Fragments arranged by size:
768 429 337 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1189 1569
Size: 1189 380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 675 710 1569
Size: 675 35 859
Fragments arranged by size:
859 675 35
DraI TTT'AAA
Cuts at: 0 443 875 1569
Size: 443 432 694
Fragments arranged by size:
694 443 432
EaeI y'GGCC_r
Cuts at: 0 78 241 1506 1569
Size: 78 163 1265 63
Fragments arranged by size:
1265 163 78 63
EarI CTCTTCn'nnn_
Cuts at: 0 326 1569
Size: 326 1243
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 152 1569
Size: 152 1417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 350 700 1017 1189 1301 1516 1569
Size: 335 15 350 317 172 112 215 53
Fragments arranged by size:
350 335 317 215 172 112 53 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1018 1569
Size: 1018 551
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 481 513 861 893 1569
Size: 481 32 348 32 676
Fragments arranged by size:
676 481 348 32 32
HincII GTy'rAC
Cuts at: 0 657 1569
Size: 657 912
HindIII A'AGCT_T
Cuts at: 0 761 1569
Size: 761 808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 1569
Size: 296 1273
MscI TGG'CCA
Cuts at: 0 243 1569
Size: 243 1326
MslI CAynn'nnrTG
Cuts at: 0 467 947 1393 1442 1569
Size: 467 480 446 49 127
Fragments arranged by size:
480 467 446 127 49
NaeI GCC'GGC
Cuts at: 0 78 1569
Size: 78 1491
NdeI CA'TA_TG
Cuts at: 0 915 1569
Size: 915 654
NgoMIV G'CCGG_C
Cuts at: 0 76 1569
Size: 76 1493
NspI r_CATG'y
Cuts at: 0 466 1569
Size: 466 1103
PacI TTA_AT'TAA
Cuts at: 0 458 1569
Size: 458 1111
PflMI CCAn_nnn'nTGG
Cuts at: 0 1049 1569
Size: 1049 520
PmlI CAC'GTG
Cuts at: 0 469 1569
Size: 469 1100
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 572 604 1569
Size: 572 32 965
Fragments arranged by size:
965 572 32
SexAI A'CCwGG_T
Cuts at: 0 1331 1569
Size: 1331 238
SfcI C'TryA_G
Cuts at: 0 908 1569
Size: 908 661
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1081 1502 1569
Size: 1081 421 67
Fragments arranged by size:
1081 421 67
TatI w'GTAC_w
Cuts at: 0 1286 1569
Size: 1286 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 270 302 1569
Size: 270 32 1267
Fragments arranged by size:
1267 270 32
Tth111I GACn'n_nGTC
Cuts at: 0 1333 1569
Size: 1333 236
XbaI T'CTAG_A
Cuts at: 0 973 1569
Size: 973 596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1353 1478 1569
Size: 1353 125 91
Fragments arranged by size:
1353 125 91
XmnI GAAnn'nnTTC
Cuts at: 0 896 998 1569
Size: 896 102 571
Fragments arranged by size:
896 571 102
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaLI ApoI AvaI BamHI
BanI BbsI BbvCI BcgI BclI BglII Bme1580I BplI
BpmI Bpu10I BsaI BsaAI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstYI BtgZI
CspCI DraI EaeI EarI EciI Eco57MI FalI Hin4I
HincII HindIII MmeI MscI MslI NaeI NdeI NgoMIV
NspI PacI PflMI PmlI PsrI SexAI SfcI TaqII
TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BanII BbeI BciVI BfrBI BglI BlpI BmgBI
BmrI BmtI BpuEI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtsI ClaI
DraIII DrdI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MspA1I NarI NcoI NheI NotI NruI NsiI
PasI PciI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI XhoI XmaI ZraI