Restriction map for
pFN21AE0998
(Linear) (Six-base) MAPSORT of: KIEE0998.seq Check: 3157 from: 1 to: 3498
KIEE0998.orf
With 182 enzymes: SgfI *
July 3, 2012 16:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1653 3422 3498
Size: 1653 1769 76
Fragments arranged by size:
1769 1653 76
AatII G_ACGT'C
Cuts at: 0 1133 3498
Size: 1133 2365
AccI GT'mk_AC
Cuts at: 0 362 1610 3498
Size: 362 1248 1888
Fragments arranged by size:
1888 1248 362
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 485 976 1303 1394 3108 3498
Size: 106 379 491 327 91 1714 390
Fragments arranged by size:
1714 491 390 379 327 106 91
AfeI AGC'GCT
Cuts at: 0 1762 3498
Size: 1762 1736
AflII C'TTAA_G
Cuts at: 0 2653 3498
Size: 2653 845
AleI CACnn'nnGTG
Cuts at: 0 310 893 3498
Size: 310 583 2605
Fragments arranged by size:
2605 583 310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2258 3498
Size: 2258 1240
AlwNI CAG_nnn'CTG
Cuts at: 0 912 2337 3205 3498
Size: 912 1425 868 293
Fragments arranged by size:
1425 912 868 293
ApaI G_GGCC'C
Cuts at: 0 228 2285 3308 3498
Size: 228 2057 1023 190
Fragments arranged by size:
2057 1023 228 190
ApaLI G'TGCA_C
Cuts at: 0 30 3498
Size: 30 3468
ApoI r'AATT_y
Cuts at: 0 2847 3498
Size: 2847 651
AvaI C'yCGr_G
Cuts at: 0 182 221 352 920 1054 1228 1273 1413
Size: 182 39 131 568 134 174 45 140
Cuts at: 1413 2097 2316 2376 2523 3498
Size: 684 219 60 147 975
Fragments arranged by size:
975 684 568 219 182 174 147 140
134 131 60 45 39
AvrII C'CTAG_G
Cuts at: 0 2967 3498
Size: 2967 531
BanI G'GyrC_C
Cuts at: 0 22 63 300 430 811 1112 1838 2131
Size: 22 41 237 130 381 301 726 293
Cuts at: 2131 2446 2464 2568 2752 3498
Size: 315 18 104 184 746
Fragments arranged by size:
746 726 381 315 301 293 237 184
130 104 41 22 18
BanII G_rGCy'C
Cuts at: 0 228 416 637 2285 2813 2944 2957 3014
Size: 228 188 221 1648 528 131 13 57
Cuts at: 3014 3308 3498
Size: 294 190
Fragments arranged by size:
1648 528 294 228 221 190 188 131
57 13
BbeI G_GCGC'C
Cuts at: 0 67 1116 1842 2135 2756 3498
Size: 67 1049 726 293 621 742
Fragments arranged by size:
1049 742 726 621 293 67
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 404 964 1784 1822 3498
Size: 248 156 560 820 38 1676
Fragments arranged by size:
1676 820 560 248 156 38
BbvCI CC'TCA_GC
Cuts at: 0 17 688 2490 3498
Size: 17 671 1802 1008
Fragments arranged by size:
1802 1008 671 17
BciVI GTATCCnnnnn_n'
Cuts at: 0 675 3498
Size: 675 2823
BglI GCCn_nnn'nGGC
Cuts at: 0 2275 2342 2548 2638 2983 3214 3498
Size: 2275 67 206 90 345 231 284
Fragments arranged by size:
2275 345 284 231 206 90 67
BglII A'GATC_T
Cuts at: 0 2312 3498
Size: 2312 1186
BlpI GC'TnA_GC
Cuts at: 0 112 3498
Size: 112 3386
Bme1580I G_kGCm'C
Cuts at: 0 25 34 228 435 814 1211 1218 2285
Size: 25 9 194 207 379 397 7 1067
Cuts at: 2285 2449 3308 3332 3498
Size: 164 859 24 166
Fragments arranged by size:
1067 859 397 379 207 194 166 164
25 24 9 7
BmgBI CAC'GTC
Cuts at: 0 1521 1694 1701 3498
Size: 1521 173 7 1797
Fragments arranged by size:
1797 1521 173 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 1205 2279 3498
Size: 1205 1074 1219
Fragments arranged by size:
1219 1205 1074
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 432 531 771 2613 3498
Size: 432 99 240 1842 885
Fragments arranged by size:
1842 885 432 240 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 457 874 1042 2190 2552 3319 3466
Size: 361 96 417 168 1148 362 767 147
Cuts at: 3466 3498
Size: 32
Fragments arranged by size:
1148 767 417 362 361 168 147 96
32
Bpu10I CC'TnA_GC
Cuts at: 0 17 688 2191 2212 2490 3498
Size: 17 671 1503 21 278 1008
Fragments arranged by size:
1503 1008 671 278 21 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 3498
Size: 721 2777
BsaI GGTCTCn'nnnn_
Cuts at: 0 850 3223 3498
Size: 850 2373 275
Fragments arranged by size:
2373 850 275
BsaHI Gr'CG_yC
Cuts at: 0 64 146 1113 1130 1839 2132 2753 3498
Size: 64 82 967 17 709 293 621 745
Fragments arranged by size:
967 745 709 621 293 82 64 17
BsaWI w'CCGG_w
Cuts at: 0 3167 3261 3498
Size: 3167 94 237
Fragments arranged by size:
3167 237 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 136 166 520 550 619 649 1948 1978
Size: 136 30 354 30 69 30 1299 30
Cuts at: 1978 2077 2107 2283 2313 2758 2788 3222 3252
Size: 99 30 176 30 445 30 434 30
Cuts at: 3252 3498
Size: 246
Fragments arranged by size:
1299 445 434 354 246 176 136 99
69 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 307 424 454 504 648 733 799 1969
Size: 307 117 30 50 144 85 66 1170
Cuts at: 1969 2085 2167 3194 3498
Size: 116 82 1027 304
Fragments arranged by size:
1170 1027 307 304 144 117 116 85
82 66 50 30
BseYI C'CCAG_C
Cuts at: 0 484 769 835 1177 1219 1736 2106 2127
Size: 484 285 66 342 42 517 370 21
Cuts at: 2127 2278 2452 2700 2812 3313 3396 3498
Size: 151 174 248 112 501 83 102
Fragments arranged by size:
517 501 484 370 342 285 248 174
151 112 102 83 66 42 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 405 610 1024 1833 3498
Size: 391 14 205 414 809 1665
Fragments arranged by size:
1665 809 414 391 205 14
BsiEI CG_ry'CG
Cuts at: 0 3224 3498
Size: 3224 274
BsiHKAI G_wGCw'C
Cuts at: 0 34 994 1466 1645 1693 2150 2431 2749
Size: 34 960 472 179 48 457 281 318
Cuts at: 2749 3498
Size: 749
Fragments arranged by size:
960 749 472 457 318 281 179 48
34
BsmI GAATG_Cn'
Cuts at: 0 3115 3498
Size: 3115 383
BsmBI CGTCTCn'nnnn_
Cuts at: 0 243 1332 3498
Size: 243 1089 2166
Fragments arranged by size:
2166 1089 243
Bsp1286I G_dGCh'C
Cuts at: 0 25 34 228 416 435 637 814 994
Size: 25 9 194 188 19 202 177 180
Cuts at: 994 1211 1218 1466 1645 1693 2150 2285 2431
Size: 217 7 248 179 48 457 135 146
Cuts at: 2431 2449 2749 2813 2944 2957 3014 3308 3332
Size: 18 300 64 131 13 57 294 24
Cuts at: 3332 3498
Size: 166
Fragments arranged by size:
457 300 294 248 217 202 194 188
180 179 177 166 146 135 131 64
57 48 25 24 19 18 13 9
7
BspEI T'CCGG_A
Cuts at: 0 3167 3498
Size: 3167 331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1046 1653 2682 3023 3035 3422 3498
Size: 1046 607 1029 341 12 387 76
Fragments arranged by size:
1046 1029 607 387 341 76 12
BsrBI CCG'CTC
Cuts at: 0 789 2093 3310 3498
Size: 789 1304 1217 188
Fragments arranged by size:
1304 1217 789 188
BsrFI r'CCGG_y
Cuts at: 0 148 297 762 961 2017 2081 2749 3498
Size: 148 149 465 199 1056 64 668 749
Fragments arranged by size:
1056 749 668 465 199 149 148 64
BsrGI T'GTAC_A
Cuts at: 0 1682 1979 3498
Size: 1682 297 1519
Fragments arranged by size:
1682 1519 297
BssHII G'CGCG_C
Cuts at: 0 2102 3116 3498
Size: 2102 1014 382
Fragments arranged by size:
2102 1014 382
BssSI C'ACGA_G
Cuts at: 0 1506 1686 1711 2149 3498
Size: 1506 180 25 438 1349
Fragments arranged by size:
1506 1349 438 180 25
BstAPI GCAn_nnn'nTGC
Cuts at: 0 29 3498
Size: 29 3469
BstEII G'GTnAC_C
Cuts at: 0 2694 3498
Size: 2694 804
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1429 1633 2460 3498
Size: 1429 204 827 1038
Fragments arranged by size:
1429 1038 827 204
BstYI r'GATC_y
Cuts at: 0 3 2312 2594 3392 3498
Size: 3 2309 282 798 106
Fragments arranged by size:
2309 798 282 106 3
BtgI C'CryG_G
Cuts at: 0 59 1316 1617 2421 2546 3242 3278 3284
Size: 59 1257 301 804 125 696 36 6
Cuts at: 3284 3498
Size: 214
Fragments arranged by size:
1257 804 696 301 214 125 59 36
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 158 404 1071 3498
Size: 158 246 667 2427
Fragments arranged by size:
2427 667 246 158
BtsI GCAGTG_nn'
Cuts at: 0 204 263 900 1470 1653 1830 2004 2261
Size: 204 59 637 570 183 177 174 257
Cuts at: 2261 3093 3498
Size: 832 405
Fragments arranged by size:
832 637 570 405 257 204 183 177
174 59
DraIII CAC_nnn'GTG
Cuts at: 0 29 3099 3498
Size: 29 3070 399
Fragments arranged by size:
3070 399 29
DrdI GACnn_nn'nnGTC
Cuts at: 0 1606 3498
Size: 1606 1892
EaeI y'GGCC_r
Cuts at: 0 599 760 1431 2418 2630 2829 3060 3221
Size: 599 161 671 987 212 199 231 161
Cuts at: 3221 3281 3498
Size: 60 217
Fragments arranged by size:
987 671 599 231 217 212 199 161
161 60
EagI C'GGCC_G
Cuts at: 0 3221 3498
Size: 3221 277
EarI CTCTTCn'nnn_
Cuts at: 0 1051 1633 3127 3498
Size: 1051 582 1494 371
Fragments arranged by size:
1494 1051 582 371
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 336 1157 1516 2175 2941 3498
Size: 336 821 359 659 766 557
Fragments arranged by size:
821 766 659 557 359 336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 361 457 485 874 976 1042 1303
Size: 106 255 96 28 389 102 66 261
Cuts at: 1303 1394 2190 2552 3108 3319 3466 3498
Size: 91 796 362 556 211 147 32
Fragments arranged by size:
796 556 389 362 261 255 211 147
106 102 96 91 66 32 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2971 3079 3498
Size: 2971 108 419
Fragments arranged by size:
2971 419 108
EcoO109I rG'GnC_Cy
Cuts at: 0 225 336 1153 2334 2610 2761 3304 3498
Size: 225 111 817 1181 276 151 543 194
Fragments arranged by size:
1181 817 543 276 225 194 151 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 630 954 1308 1947 3105 3498
Size: 630 324 354 639 1158 393
Fragments arranged by size:
1158 639 630 393 354 324
FseI GG_CCGG'CC
Cuts at: 0 766 3498
Size: 766 2732
FspI TGC'GCA
Cuts at: 0 3031 3498
Size: 3031 467
HaeII r_GCGC'y
Cuts at: 0 67 1116 1226 1764 1842 2082 2135 2219
Size: 67 1049 110 538 78 240 53 84
Cuts at: 2219 2756 2989 3498
Size: 537 233 509
Fragments arranged by size:
1049 538 537 509 240 233 110 84
78 67 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 2043 2075 3222 3254 3498
Size: 136 32 1875 32 1147 32 244
Fragments arranged by size:
1875 1147 244 136 32 32 32
HincII GTy'rAC
Cuts at: 0 1289 2178 3498
Size: 1289 889 1320
Fragments arranged by size:
1320 1289 889
KasI G'GCGC_C
Cuts at: 0 63 1112 1838 2131 2752 3498
Size: 63 1049 726 293 621 746
Fragments arranged by size:
1049 746 726 621 293 63
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2396 3498
Size: 2396 1102
MscI TGG'CCA
Cuts at: 0 601 1433 2632 2831 3498
Size: 601 832 1199 199 667
Fragments arranged by size:
1199 832 667 601 199
MslI CAynn'nnrTG
Cuts at: 0 310 893 1631 1663 3498
Size: 310 583 738 32 1835
Fragments arranged by size:
1835 738 583 310 32
MspA1I CmG'CkG
Cuts at: 0 16 479 1593 2647 2947 3363 3498
Size: 16 463 1114 1054 300 416 135
Fragments arranged by size:
1114 1054 463 416 300 135 16
NaeI GCC'GGC
Cuts at: 0 150 299 764 2019 2083 3498
Size: 150 149 465 1255 64 1415
Fragments arranged by size:
1415 1255 465 150 149 64
NarI GG'CG_CC
Cuts at: 0 64 1113 1839 2132 2753 3498
Size: 64 1049 726 293 621 745
Fragments arranged by size:
1049 745 726 621 293 64
NcoI C'CATG_G
Cuts at: 0 59 3242 3498
Size: 59 3183 256
Fragments arranged by size:
3183 256 59
NgoMIV G'CCGG_C
Cuts at: 0 148 297 762 2017 2081 3498
Size: 148 149 465 1255 64 1417
Fragments arranged by size:
1417 1255 465 149 148 64
NspI r_CATG'y
Cuts at: 0 564 1542 1662 3498
Size: 564 978 120 1836
Fragments arranged by size:
1836 978 564 120
PasI CC'CwG_GG
Cuts at: 0 378 2331 3498
Size: 378 1953 1167
Fragments arranged by size:
1953 1167 378
PflMI CCAn_nnn'nTGG
Cuts at: 0 126 3099 3498
Size: 126 2973 399
Fragments arranged by size:
2973 399 126
PfoI T'CCnGG_A
Cuts at: 0 3488 3498
Size: 3488 10
PshAI GACnn'nnGTC
Cuts at: 0 2786 3498
Size: 2786 712
PspOMI G'GGCC_C
Cuts at: 0 224 2281 3304 3498
Size: 224 2057 1023 194
Fragments arranged by size:
2057 1023 224 194
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 268 300 3498
Size: 268 32 3198
Fragments arranged by size:
3198 268 32
PstI C_TGCA'G
Cuts at: 0 48 1568 1606 1837 2646 3498
Size: 48 1520 38 231 809 852
Fragments arranged by size:
1520 852 809 231 48 38
PvuII CAG'CTG
Cuts at: 0 16 2647 3363 3498
Size: 16 2631 716 135
Fragments arranged by size:
2631 716 135 16
SapI GCTCTTCn'nnn_
Cuts at: 0 1633 3127 3498
Size: 1633 1494 371
Fragments arranged by size:
1633 1494 371
SbfI CC_TGCA'GG
Cuts at: 0 48 3498
Size: 48 3450
SexAI A'CCwGG_T
Cuts at: 0 911 3498
Size: 911 2587
SfcI C'TryA_G
Cuts at: 0 44 1564 1602 1833 2642 3498
Size: 44 1520 38 231 809 856
Fragments arranged by size:
1520 856 809 231 44 38
SfoI GGC'GCC
Cuts at: 0 65 1114 1840 2133 2754 3498
Size: 65 1049 726 293 621 744
Fragments arranged by size:
1049 744 726 621 293 65
SgrAI Cr'CCGG_yG
Cuts at: 0 2749 3498
Size: 2749 749
SmaI CCC'GGG
Cuts at: 0 184 223 922 1056 1230 3498
Size: 184 39 699 134 174 2268
Fragments arranged by size:
2268 699 184 174 134 39
SmlI C'TyrA_G
Cuts at: 0 736 2653 3498
Size: 736 1917 845
Fragments arranged by size:
1917 845 736
SphI G_CATG'C
Cuts at: 0 1662 3498
Size: 1662 1836
SrfI GCCC'GGGC
Cuts at: 0 223 922 3498
Size: 223 699 2576
Fragments arranged by size:
2576 699 223
StuI AGG'CCT
Cuts at: 0 511 3498
Size: 511 2987
StyI C'CwwG_G
Cuts at: 0 59 419 467 860 1160 1922 2135 2967
Size: 59 360 48 393 300 762 213 832
Cuts at: 2967 3131 3242 3428 3498
Size: 164 111 186 70
Fragments arranged by size:
832 762 393 360 300 213 186 164
111 70 59 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 261 318 562 796 1660 2452 2523 3498
Size: 261 57 244 234 864 792 71 975
Fragments arranged by size:
975 864 792 261 244 234 71 57
TatI w'GTAC_w
Cuts at: 0 284 722 1682 1979 3498
Size: 284 438 960 297 1519
Fragments arranged by size:
1519 960 438 297 284
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 619 651 2077 2109 2140 2172 2283 2315
Size: 619 32 1426 32 31 32 111 32
Cuts at: 2315 2758 2790 3498
Size: 443 32 708
Fragments arranged by size:
1426 708 619 443 111 32 32 32
32 32 31
XbaI T'CTAG_A
Cuts at: 0 2790 3444 3498
Size: 2790 654 54
Fragments arranged by size:
2790 654 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 196 312 1017 1179 2338 3240 3498
Size: 196 116 705 162 1159 902 258
Fragments arranged by size:
1159 902 705 258 196 162 116
XmaI C'CCGG_G
Cuts at: 0 182 221 920 1054 1228 3498
Size: 182 39 699 134 174 2270
Fragments arranged by size:
2270 699 182 174 134 39
ZraI GAC'GTC
Cuts at: 0 1131 3498
Size: 1131 2367
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII
BbeI BbsI BbvCI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FalI FseI FspI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PshAI PspOMI PsrI PstI
PvuII SapI SbfI SexAI SfcI SfoI SgrAI SmaI
SmlI SphI SrfI StuI StyI TaqII TatI TstI
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflIII AgeI AhdI AloI AscI AseI
AsiSI BaeI BamHI BcgI BclI BfrBI BmtI BsaAI
BsaBI BsiWI BspHI BsrDI BstBI BstZ17I Bsu36I ClaI
CspCI DraI EcoICRI EcoRI EcoRV FspAI HindIII HpaI
KpnI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PpuMI PsiI PspXI
PvuI RsrII SacI SacII SalI SanDI ScaI SfiI
SgfI SnaBI SpeI SspI SwaI Tth111I XhoI XmnI