Restriction map for
pFN21AE0992
(Linear) (Six-base) MAPSORT of: KIEE0992.seq Check: 3267 from: 1 to: 1893
KIEE0992.orf
With 182 enzymes: SgfI *
March 22, 2012 15:50 ..
AccI GT'mk_AC
Cuts at: 0 399 1893
Size: 399 1494
Acc65I G'GTAC_C
Cuts at: 0 1025 1893
Size: 1025 868
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1085 1608 1622 1893
Size: 1085 523 14 271
Fragments arranged by size:
1085 523 271 14
ApaLI G'TGCA_C
Cuts at: 0 1293 1893
Size: 1293 600
ApoI r'AATT_y
Cuts at: 0 3 367 1353 1893
Size: 3 364 986 540
Fragments arranged by size:
986 540 364 3
AvaI C'yCGr_G
Cuts at: 0 1736 1893
Size: 1736 157
BanI G'GyrC_C
Cuts at: 0 1025 1893
Size: 1025 868
BanII G_rGCy'C
Cuts at: 0 1173 1893
Size: 1173 720
BbsI GAAGACnn'nnnn_
Cuts at: 0 64 1893
Size: 64 1829
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1257 1291 1893
Size: 1257 34 602
Fragments arranged by size:
1257 602 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 242 1893
Size: 242 1651
BclI T'GATC_A
Cuts at: 0 1548 1893
Size: 1548 345
BglII A'GATC_T
Cuts at: 0 1464 1839 1893
Size: 1464 375 54
Fragments arranged by size:
1464 375 54
BlpI GC'TnA_GC
Cuts at: 0 1399 1893
Size: 1399 494
Bme1580I G_kGCm'C
Cuts at: 0 1297 1893
Size: 1297 596
BmrI ACTGGGnnnn_n'
Cuts at: 0 1024 1167 1893
Size: 1024 143 726
Fragments arranged by size:
1024 726 143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 1459 1893
Size: 458 1001 434
Fragments arranged by size:
1001 458 434
BsaAI yAC'GTr
Cuts at: 0 699 1893
Size: 699 1194
BsaHI Gr'CG_yC
Cuts at: 0 1768 1893
Size: 1768 125
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 832 862 906 936 1893
Size: 832 30 44 30 957
Fragments arranged by size:
957 832 44 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 769 884 1234 1893
Size: 37 732 115 350 659
Fragments arranged by size:
732 659 350 115 37
BseYI C'CCAG_C
Cuts at: 0 1298 1893
Size: 1298 595
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1297 1893
Size: 1297 596
BsiHKAI G_wGCw'C
Cuts at: 0 1297 1893
Size: 1297 596
BsmI GAATG_Cn'
Cuts at: 0 1056 1694 1893
Size: 1056 638 199
Fragments arranged by size:
1056 638 199
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1382 1893
Size: 1382 511
Bsp1286I G_dGCh'C
Cuts at: 0 1173 1297 1893
Size: 1173 124 596
Fragments arranged by size:
1173 596 124
BspHI T'CATG_A
Cuts at: 0 249 1893
Size: 249 1644
BsrDI GCAATG_nn'
Cuts at: 0 1217 1893
Size: 1217 676
BsrGI T'GTAC_A
Cuts at: 0 839 1893
Size: 839 1054
BstBI TT'CG_AA
Cuts at: 0 1835 1893
Size: 1835 58
BstEII G'GTnAC_C
Cuts at: 0 1722 1893
Size: 1722 171
BstYI r'GATC_y
Cuts at: 0 1464 1839 1893
Size: 1464 375 54
Fragments arranged by size:
1464 375 54
BtgI C'CryG_G
Cuts at: 0 1727 1893
Size: 1727 166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 47 1893
Size: 47 1846
BtsI GCAGTG_nn'
Cuts at: 0 792 1061 1893
Size: 792 269 832
Fragments arranged by size:
832 792 269
EaeI y'GGCC_r
Cuts at: 0 1529 1893
Size: 1529 364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 1085 1459 1608 1622 1893
Size: 458 627 374 149 14 271
Fragments arranged by size:
627 458 374 271 149 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1116 1893
Size: 1116 777
EcoO109I rG'GnC_Cy
Cuts at: 0 297 1893
Size: 297 1596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 103 1847 1893
Size: 103 1744 46
Fragments arranged by size:
1744 103 46
FspI TGC'GCA
Cuts at: 0 1849 1893
Size: 1849 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 342 374 575 607 1359 1391 1392 1419
Size: 342 32 201 32 752 32 1 27
Cuts at: 1419 1424 1451 1630 1662 1893
Size: 5 27 179 32 231
Fragments arranged by size:
752 342 231 201 179 32 32 32
32 27 27 5 1
HincII GTy'rAC
Cuts at: 0 829 1893
Size: 829 1064
KpnI G_GTAC'C
Cuts at: 0 1029 1893
Size: 1029 864
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1386 1796 1893
Size: 1386 410 97
Fragments arranged by size:
1386 410 97
MscI TGG'CCA
Cuts at: 0 1531 1893
Size: 1531 362
MslI CAynn'nnrTG
Cuts at: 0 423 1893
Size: 423 1470
NcoI C'CATG_G
Cuts at: 0 1727 1893
Size: 1727 166
NspI r_CATG'y
Cuts at: 0 955 1407 1893
Size: 955 452 486
Fragments arranged by size:
955 486 452
PpuMI rG'GwC_Cy
Cuts at: 0 297 1893
Size: 297 1596
PspXI vC'TCGA_Gb
Cuts at: 0 1736 1893
Size: 1736 157
PstI C_TGCA'G
Cuts at: 0 1068 1893
Size: 1068 825
SfcI C'TryA_G
Cuts at: 0 1064 1893
Size: 1064 829
SmlI C'TyrA_G
Cuts at: 0 1736 1893
Size: 1736 157
SnaBI TAC'GTA
Cuts at: 0 699 1893
Size: 699 1194
SpeI A'CTAG_T
Cuts at: 0 1863 1893
Size: 1863 30
SspI AAT'ATT
Cuts at: 0 345 1893
Size: 345 1548
StuI AGG'CCT
Cuts at: 0 501 1827 1893
Size: 501 1326 66
Fragments arranged by size:
1326 501 66
StyI C'CwwG_G
Cuts at: 0 1727 1893
Size: 1727 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 294 1071 1312 1893
Size: 294 777 241 581
Fragments arranged by size:
777 581 294 241
TatI w'GTAC_w
Cuts at: 0 93 839 1175 1553 1893
Size: 93 746 336 378 340
Fragments arranged by size:
746 378 340 336 93
Tth111I GACn'n_nGTC
Cuts at: 0 1373 1893
Size: 1373 520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1539 1716 1893
Size: 1539 177 177
Fragments arranged by size:
1539 177 177
XhoI C'TCGA_G
Cuts at: 0 1736 1893
Size: 1736 157
XmnI GAAnn'nnTTC
Cuts at: 0 1827 1893
Size: 1827 66
Enzymes that do cut:
AccI Acc65I AcuI ApaLI ApoI AvaI BanI BanII
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BpmI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BsrDI BsrGI BstBI BstEII
BstYI BtgI BtgZI BtsI EaeI Eco57MI EcoNI EcoO109I
FalI FspI Hin4I HincII KpnI MmeI MscI MslI
NcoI NspI PpuMI PspXI PstI SfcI SmlI SnaBI
SpeI SspI StuI StyI TaqII TatI Tth111I XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BfrBI BglI BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI BsiEI
BsiWI BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI
BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoRI EcoRV FseI FspAI
HaeII HindIII HpaI KasI MfeI MluI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SphI SrfI SwaI TstI XbaI
XmaI ZraI