Restriction map for
pFN21AE0991
(Linear) (Six-base) MAPSORT of: KIEE0991.seq Check: 371 from: 1 to: 1878
KIEE0991.orf
With 182 enzymes: SgfI *
July 3, 2012 16:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1299 1878
Size: 1299 579
AccI GT'mk_AC
Cuts at: 0 212 1878
Size: 212 1666
Acc65I G'GTAC_C
Cuts at: 0 491 1878
Size: 491 1387
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 1412 1652 1710 1878
Size: 274 1138 240 58 168
Fragments arranged by size:
1138 274 240 168 58
AlwNI CAG_nnn'CTG
Cuts at: 0 772 930 1363 1493 1878
Size: 772 158 433 130 385
Fragments arranged by size:
772 433 385 158 130
ApaI G_GGCC'C
Cuts at: 0 31 38 68 1384 1611 1878
Size: 31 7 30 1316 227 267
Fragments arranged by size:
1316 267 227 31 30 7
ApaLI G'TGCA_C
Cuts at: 0 358 1878
Size: 358 1520
AvaI C'yCGr_G
Cuts at: 0 37 69 96 479 755 1127 1324 1625
Size: 37 32 27 383 276 372 197 301
Cuts at: 1625 1676 1878
Size: 51 202
Fragments arranged by size:
383 372 301 276 202 197 51 37
32 27
BamHI G'GATC_C
Cuts at: 0 938 1878
Size: 938 940
BanI G'GyrC_C
Cuts at: 0 51 224 323 491 1826 1878
Size: 51 173 99 168 1335 52
Fragments arranged by size:
1335 173 168 99 52 51
BanII G_rGCy'C
Cuts at: 0 31 38 68 129 658 749 1003 1331
Size: 31 7 30 61 529 91 254 328
Cuts at: 1331 1384 1598 1611 1685 1878
Size: 53 214 13 74 193
Fragments arranged by size:
529 328 254 214 193 91 74 61
53 31 30 13 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1160 1878
Size: 1160 718
BglI GCCn_nnn'nGGC
Cuts at: 0 72 453 1878
Size: 72 381 1425
Fragments arranged by size:
1425 381 72
BlpI GC'TnA_GC
Cuts at: 0 421 1878
Size: 421 1457
Bme1580I G_kGCm'C
Cuts at: 0 31 38 68 85 362 906 1384 1611
Size: 31 7 30 17 277 544 478 227
Cuts at: 1611 1753 1829 1872 1878
Size: 142 76 43 6
Fragments arranged by size:
544 478 277 227 142 76 43 31
30 17 7 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 266 1119 1878
Size: 266 853 759
Fragments arranged by size:
853 759 266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 659 964 1421 1660 1878
Size: 65 594 305 457 239 218
Fragments arranged by size:
594 457 305 239 218 65
Bpu10I CC'TnA_GC
Cuts at: 0 319 956 1424 1878
Size: 319 637 468 454
Fragments arranged by size:
637 468 454 319
BsaI GGTCTCn'nnnn_
Cuts at: 0 1261 1878
Size: 1261 617
BsaWI w'CCGG_w
Cuts at: 0 1263 1878
Size: 1263 615
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 341 371 484 514 1878
Size: 341 30 113 30 1364
Fragments arranged by size:
1364 341 113 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 105 1801 1878
Size: 105 1696 77
Fragments arranged by size:
1696 105 77
BseYI C'CCAG_C
Cuts at: 0 892 923 1192 1451 1554 1583 1588 1610
Size: 892 31 269 259 103 29 5 22
Cuts at: 1610 1849 1878
Size: 239 29
Fragments arranged by size:
892 269 259 239 103 31 29 29
22 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 358 481 1878
Size: 322 36 123 1397
Fragments arranged by size:
1397 322 123 36
BsiHKAI G_wGCw'C
Cuts at: 0 362 749 1331 1878
Size: 362 387 582 547
Fragments arranged by size:
582 547 387 362
Bsp1286I G_dGCh'C
Cuts at: 0 31 38 68 85 129 362 658 749
Size: 31 7 30 17 44 233 296 91
Cuts at: 749 906 1003 1331 1384 1598 1611 1685 1753
Size: 157 97 328 53 214 13 74 68
Cuts at: 1753 1829 1872 1878
Size: 76 43 6
Fragments arranged by size:
328 296 233 214 157 97 91 76
74 68 53 44 43 31 30 17
13 7 6
BspEI T'CCGG_A
Cuts at: 0 1263 1878
Size: 1263 615
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 503 771 1299 1878
Size: 503 268 528 579
Fragments arranged by size:
579 528 503 268
BsrBI CCG'CTC
Cuts at: 0 1307 1644 1878
Size: 1307 337 234
Fragments arranged by size:
1307 337 234
BsrDI GCAATG_nn'
Cuts at: 0 473 1878
Size: 473 1405
BsrFI r'CCGG_y
Cuts at: 0 193 314 447 1064 1878
Size: 193 121 133 617 814
Fragments arranged by size:
814 617 193 133 121
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1620 1878
Size: 1620 258
BstEII G'GTnAC_C
Cuts at: 0 1254 1878
Size: 1254 624
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1583 1878
Size: 1583 295
BstYI r'GATC_y
Cuts at: 0 938 1044 1878
Size: 938 106 834
Fragments arranged by size:
938 834 106
Bsu36I CC'TnA_GG
Cuts at: 0 337 1159 1878
Size: 337 822 719
Fragments arranged by size:
822 719 337
BtgI C'CryG_G
Cuts at: 0 647 1878
Size: 647 1231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 735 1878
Size: 735 1143
BtsI GCAGTG_nn'
Cuts at: 0 19 171 847 873 1351 1878
Size: 19 152 676 26 478 527
Fragments arranged by size:
676 527 478 152 26 19
EaeI y'GGCC_r
Cuts at: 0 2 445 596 644 866 1469 1878
Size: 2 443 151 48 222 603 409
Fragments arranged by size:
603 443 409 222 151 48 2
EarI CTCTTCn'nnn_
Cuts at: 0 1081 1388 1878
Size: 1081 307 490
Fragments arranged by size:
1081 490 307
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 104 1878
Size: 104 1774
EcoICRI GAG'CTC
Cuts at: 0 747 1329 1878
Size: 747 582 549
Fragments arranged by size:
747 582 549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 274 659 964 1412 1421 1652 1660
Size: 65 209 385 305 448 9 231 8
Cuts at: 1660 1710 1878
Size: 50 168
Fragments arranged by size:
448 385 305 231 209 168 65 50
9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1526 1878
Size: 1526 352
EcoO109I rG'GnC_Cy
Cuts at: 0 27 28 64 65 1018 1360 1381 1531
Size: 27 1 36 1 953 342 21 150
Cuts at: 1531 1878
Size: 347
Fragments arranged by size:
953 347 342 150 36 27 21 1
1
HaeII r_GCGC'y
Cuts at: 0 140 1450 1878
Size: 140 1310 428
Fragments arranged by size:
1310 428 140
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 514 546 978 1010 1523 1555 1878
Size: 514 32 432 32 513 32 323
Fragments arranged by size:
514 513 432 323 32 32 32
HincII GTy'rAC
Cuts at: 0 1134 1878
Size: 1134 744
KpnI G_GTAC'C
Cuts at: 0 495 1878
Size: 495 1383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 1878
Size: 573 1305
MscI TGG'CCA
Cuts at: 0 868 1471 1878
Size: 868 603 407
Fragments arranged by size:
868 603 407
MspA1I CmG'CkG
Cuts at: 0 306 372 417 574 951 1245 1301 1375
Size: 306 66 45 157 377 294 56 74
Cuts at: 1375 1455 1558 1768 1860 1878
Size: 80 103 210 92 18
Fragments arranged by size:
377 306 294 210 157 103 92 80
74 66 56 45 18
NaeI GCC'GGC
Cuts at: 0 195 1878
Size: 195 1683
NgoMIV G'CCGG_C
Cuts at: 0 193 1878
Size: 193 1685
PasI CC'CwG_GG
Cuts at: 0 31 1878
Size: 31 1847
PflMI CCAn_nnn'nTGG
Cuts at: 0 930 1363 1878
Size: 930 433 515
Fragments arranged by size:
930 515 433
PfoI T'CCnGG_A
Cuts at: 0 941 1878
Size: 941 937
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 404 436 1878
Size: 404 32 1442
Fragments arranged by size:
1442 404 32
PpuMI rG'GwC_Cy
Cuts at: 0 1018 1360 1531 1878
Size: 1018 342 171 347
Fragments arranged by size:
1018 347 342 171
PshAI GACnn'nnGTC
Cuts at: 0 1129 1273 1878
Size: 1129 144 605
Fragments arranged by size:
1129 605 144
PspOMI G'GGCC_C
Cuts at: 0 27 34 64 1380 1607 1878
Size: 27 7 30 1316 227 271
Fragments arranged by size:
1316 271 227 30 27 7
PspXI vC'TCGA_Gb
Cuts at: 0 1127 1878
Size: 1127 751
PstI C_TGCA'G
Cuts at: 0 768 1859 1878
Size: 768 1091 19
Fragments arranged by size:
1091 768 19
PvuII CAG'CTG
Cuts at: 0 306 372 417 951 1245 1455 1558 1768
Size: 306 66 45 534 294 210 103 210
Cuts at: 1768 1860 1878
Size: 92 18
Fragments arranged by size:
534 306 294 210 210 103 92 66
45 18
SacI G_AGCT'C
Cuts at: 0 749 1331 1878
Size: 749 582 547
Fragments arranged by size:
749 582 547
SanDI GG'GwC_CC
Cuts at: 0 1018 1878
Size: 1018 860
SfcI C'TryA_G
Cuts at: 0 764 1855 1878
Size: 764 1091 23
Fragments arranged by size:
1091 764 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 72 1878
Size: 72 1806
SmaI CCC'GGG
Cuts at: 0 39 71 98 1627 1678 1878
Size: 39 32 27 1529 51 200
Fragments arranged by size:
1529 200 51 39 32 27
SmlI C'TyrA_G
Cuts at: 0 1127 1878
Size: 1127 751
SrfI GCCC'GGGC
Cuts at: 0 39 98 1878
Size: 39 59 1780
Fragments arranged by size:
1780 59 39
StuI AGG'CCT
Cuts at: 0 485 1878
Size: 485 1393
StyI C'CwwG_G
Cuts at: 0 1250 1878
Size: 1250 628
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 571 1206 1857 1863 1878
Size: 571 635 651 6 15
Fragments arranged by size:
651 635 571 15 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 341 373 1878
Size: 341 32 1505
Fragments arranged by size:
1505 341 32
XhoI C'TCGA_G
Cuts at: 0 1127 1878
Size: 1127 751
XmaI C'CCGG_G
Cuts at: 0 37 69 96 1625 1676 1878
Size: 37 32 27 1529 51 202
Fragments arranged by size:
1529 202 51 37 32 27
XmnI GAAnn'nnTTC
Cuts at: 0 605 1878
Size: 605 1273
Enzymes that do cut:
AarI AccI Acc65I AcuI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbsI BglI BlpI Bme1580I BmrI
BpmI Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII KpnI MmeI MscI MspA1I NaeI NgoMIV PasI
PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SanDI SfcI SfiI SmaI SmlI SrfI
StuI StyI TaqII TstI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI
BmtI BplI BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI
BsmI BsmBI BspHI BsrGI BssHII BssSI BstBI BstZ17I
ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI MfeI
MluI MslI NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PsiI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
TatI Tth111I XbaI XcmI ZraI