Restriction map for
pFN21AE0987
(Linear) (Six-base) MAPSORT of: KIEE0987.seq Check: 7509 from: 1 to: 1848
KIEE0987.orf
With 182 enzymes: SgfI *
July 3, 2012 16:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 345 1482 1848
Size: 345 1137 366
Fragments arranged by size:
1137 366 345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 221 438 1485 1848
Size: 187 34 217 1047 363
Fragments arranged by size:
1047 363 217 187 34
AflIII A'CryG_T
Cuts at: 0 590 1848
Size: 590 1258
AhdI GACnn_n'nnGTC
Cuts at: 0 594 1848
Size: 594 1254
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 865 1848
Size: 865 983
AlwNI CAG_nnn'CTG
Cuts at: 0 696 1053 1550 1565 1678 1700 1848
Size: 696 357 497 15 113 22 148
Fragments arranged by size:
696 497 357 148 113 22 15
ApaI G_GGCC'C
Cuts at: 0 549 1411 1637 1756 1848
Size: 549 862 226 119 92
Fragments arranged by size:
862 549 226 119 92
AvaI C'yCGr_G
Cuts at: 0 1404 1585 1805 1848
Size: 1404 181 220 43
Fragments arranged by size:
1404 220 181 43
BamHI G'GATC_C
Cuts at: 0 478 1848
Size: 478 1370
BanI G'GyrC_C
Cuts at: 0 1254 1841 1848
Size: 1254 587 7
Fragments arranged by size:
1254 587 7
BanII G_rGCy'C
Cuts at: 0 549 1411 1418 1637 1685 1756 1764 1772
Size: 549 862 7 219 48 71 8 8
Cuts at: 1772 1848
Size: 76
Fragments arranged by size:
862 549 219 76 71 48 8 8
7
BbvCI CC'TCA_GC
Cuts at: 0 1187 1848
Size: 1187 661
BclI T'GATC_A
Cuts at: 0 968 1848
Size: 968 880
BglI GCCn_nnn'nGGC
Cuts at: 0 730 1448 1848
Size: 730 718 400
Fragments arranged by size:
730 718 400
BlpI GC'TnA_GC
Cuts at: 0 617 1519 1598 1848
Size: 617 902 79 250
Fragments arranged by size:
902 617 250 79
Bme1580I G_kGCm'C
Cuts at: 0 549 1065 1400 1411 1637 1756 1844 1848
Size: 549 516 335 11 226 119 88 4
Fragments arranged by size:
549 516 335 226 119 88 11 4
BmgBI CAC'GTC
Cuts at: 0 213 591 1848
Size: 213 378 1257
Fragments arranged by size:
1257 378 213
BmrI ACTGGGnnnn_n'
Cuts at: 0 1007 1848
Size: 1007 841
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1600 1848
Size: 1600 248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1727 1759 1810 1848
Size: 295 1432 32 51 38
Fragments arranged by size:
1432 295 51 38 32
Bpu10I CC'TnA_GC
Cuts at: 0 1187 1412 1679 1763 1848
Size: 1187 225 267 84 85
Fragments arranged by size:
1187 267 225 85 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 232 247 364 394 772 838 934
Size: 109 123 15 117 30 378 66 96
Cuts at: 934 1354 1848
Size: 420 494
Fragments arranged by size:
494 420 378 123 117 109 96 66
30 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 1063 1324 1353 1533 1848
Size: 1063 261 29 180 315
Fragments arranged by size:
1063 315 261 180 29
BsaAI yAC'GTr
Cuts at: 0 510 1848
Size: 510 1338
BsaHI Gr'CG_yC
Cuts at: 0 134 242 1848
Size: 134 108 1606
Fragments arranged by size:
1606 134 108
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1204 1261 1848
Size: 1204 57 587
Fragments arranged by size:
1204 587 57
BseYI C'CCAG_C
Cuts at: 0 561 1309 1565 1693 1848
Size: 561 748 256 128 155
Fragments arranged by size:
748 561 256 155 128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 607 1320 1716 1848
Size: 160 447 713 396 132
Fragments arranged by size:
713 447 396 160 132
BsiHKAI G_wGCw'C
Cuts at: 0 97 362 941 1685 1848
Size: 97 265 579 744 163
Fragments arranged by size:
744 579 265 163 97
BsmBI CGTCTCn'nnnn_
Cuts at: 0 808 1848
Size: 808 1040
Bsp1286I G_dGCh'C
Cuts at: 0 97 362 549 941 1065 1400 1411 1418
Size: 97 265 187 392 124 335 11 7
Cuts at: 1418 1637 1685 1756 1764 1772 1844 1848
Size: 219 48 71 8 8 72 4
Fragments arranged by size:
392 335 265 219 187 124 97 72
71 48 11 8 8 7 4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 345 1342 1482 1848
Size: 345 997 140 366
Fragments arranged by size:
997 366 345 140
BsrBI CCG'CTC
Cuts at: 0 1784 1848
Size: 1784 64
BsrFI r'CCGG_y
Cuts at: 0 1222 1848
Size: 1222 626
BssSI C'ACGA_G
Cuts at: 0 1066 1848
Size: 1066 782
BstAPI GCAn_nnn'nTGC
Cuts at: 0 183 1053 1848
Size: 183 870 795
Fragments arranged by size:
870 795 183
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1795 1848
Size: 1795 53
BstYI r'GATC_y
Cuts at: 0 119 478 554 1848
Size: 119 359 76 1294
Fragments arranged by size:
1294 359 119 76
Bsu36I CC'TnA_GG
Cuts at: 0 1535 1848
Size: 1535 313
BtgI C'CryG_G
Cuts at: 0 1655 1756 1848
Size: 1655 101 92
Fragments arranged by size:
1655 101 92
BtsI GCAGTG_nn'
Cuts at: 0 177 897 1065 1221 1848
Size: 177 720 168 156 627
Fragments arranged by size:
720 627 177 168 156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 695 730 1848
Size: 695 35 1118
Fragments arranged by size:
1118 695 35
DraIII CAC_nnn'GTG
Cuts at: 0 1395 1848
Size: 1395 453
DrdI GACnn_nn'nnGTC
Cuts at: 0 990 1360 1848
Size: 990 370 488
Fragments arranged by size:
990 488 370
EaeI y'GGCC_r
Cuts at: 0 110 809 896 1151 1220 1386 1607 1848
Size: 110 699 87 255 69 166 221 241
Fragments arranged by size:
699 255 241 221 166 110 87 69
EarI CTCTTCn'nnn_
Cuts at: 0 197 1690 1848
Size: 197 1493 158
Fragments arranged by size:
1493 197 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 105 471 949 1848
Size: 105 366 478 899
Fragments arranged by size:
899 478 366 105
EcoICRI GAG'CTC
Cuts at: 0 1683 1848
Size: 1683 165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 221 295 438 1485 1727 1759 1810
Size: 187 34 74 143 1047 242 32 51
Cuts at: 1810 1848
Size: 38
Fragments arranged by size:
1047 242 187 143 74 51 38 34
32
EcoO109I rG'GnC_Cy
Cuts at: 0 59 546 693 1166 1408 1547 1589 1752
Size: 59 487 147 473 242 139 42 163
Cuts at: 1752 1848
Size: 96
Fragments arranged by size:
487 473 242 163 147 139 96 59
42
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 78 1848
Size: 78 1770
FspI TGC'GCA
Cuts at: 0 1057 1099 1848
Size: 1057 42 749
Fragments arranged by size:
1057 749 42
HaeII r_GCGC'y
Cuts at: 0 840 931 1848
Size: 840 91 917
Fragments arranged by size:
917 840 91
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 580 612 1848
Size: 463 32 85 32 1236
Fragments arranged by size:
1236 463 85 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 1376 1514 1520 1848
Size: 842 534 138 6 328
Fragments arranged by size:
842 534 328 138 6
MscI TGG'CCA
Cuts at: 0 112 898 1388 1609 1848
Size: 112 786 490 221 239
Fragments arranged by size:
786 490 239 221 112
MslI CAynn'nnrTG
Cuts at: 0 430 1654 1793 1848
Size: 430 1224 139 55
Fragments arranged by size:
1224 430 139 55
MspA1I CmG'CkG
Cuts at: 0 446 1053 1284 1565 1700 1848
Size: 446 607 231 281 135 148
Fragments arranged by size:
607 446 281 231 148 135
NaeI GCC'GGC
Cuts at: 0 1224 1848
Size: 1224 624
NcoI C'CATG_G
Cuts at: 0 1655 1848
Size: 1655 193
NgoMIV G'CCGG_C
Cuts at: 0 1222 1848
Size: 1222 626
NspI r_CATG'y
Cuts at: 0 186 981 1834 1848
Size: 186 795 853 14
Fragments arranged by size:
853 795 186 14
PasI CC'CwG_GG
Cuts at: 0 600 690 1848
Size: 600 90 1158
Fragments arranged by size:
1158 600 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 83 779 1848
Size: 83 696 1069
Fragments arranged by size:
1069 696 83
PfoI T'CCnGG_A
Cuts at: 0 1704 1848
Size: 1704 144
PpuMI rG'GwC_Cy
Cuts at: 0 59 1166 1848
Size: 59 1107 682
Fragments arranged by size:
1107 682 59
PshAI GACnn'nnGTC
Cuts at: 0 1295 1848
Size: 1295 553
PspOMI G'GGCC_C
Cuts at: 0 545 1407 1633 1752 1848
Size: 545 862 226 119 96
Fragments arranged by size:
862 545 226 119 96
PstI C_TGCA'G
Cuts at: 0 356 1304 1619 1848
Size: 356 948 315 229
Fragments arranged by size:
948 356 315 229
PvuII CAG'CTG
Cuts at: 0 1053 1284 1565 1700 1848
Size: 1053 231 281 135 148
Fragments arranged by size:
1053 281 231 148 135
SacI G_AGCT'C
Cuts at: 0 1685 1848
Size: 1685 163
SanDI GG'GwC_CC
Cuts at: 0 1166 1848
Size: 1166 682
SapI GCTCTTCn'nnn_
Cuts at: 0 1690 1848
Size: 1690 158
SbfI CC_TGCA'GG
Cuts at: 0 356 1848
Size: 356 1492
SexAI A'CCwGG_T
Cuts at: 0 257 665 1257 1848
Size: 257 408 592 591
Fragments arranged by size:
592 591 408 257
SfcI C'TryA_G
Cuts at: 0 352 1300 1431 1615 1848
Size: 352 948 131 184 233
Fragments arranged by size:
948 352 233 184 131
SmaI CCC'GGG
Cuts at: 0 1807 1848
Size: 1807 41
SmlI C'TyrA_G
Cuts at: 0 124 226 247 379 409 787 853 949
Size: 124 102 21 132 30 378 66 96
Cuts at: 949 1369 1848
Size: 420 479
Fragments arranged by size:
479 420 378 132 124 102 96 66
30 21
SphI G_CATG'C
Cuts at: 0 186 981 1848
Size: 186 795 867
Fragments arranged by size:
867 795 186
StuI AGG'CCT
Cuts at: 0 191 1848
Size: 191 1657
StyI C'CwwG_G
Cuts at: 0 77 1655 1848
Size: 77 1578 193
Fragments arranged by size:
1578 193 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1090 1848
Size: 1090 758
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1595 1627 1848
Size: 1595 32 221
Fragments arranged by size:
1595 221 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1146 1281 1848
Size: 1146 135 567
Fragments arranged by size:
1146 567 135
XmaI C'CCGG_G
Cuts at: 0 1805 1848
Size: 1805 43
XmnI GAAnn'nnTTC
Cuts at: 0 310 1848
Size: 310 1538
Enzymes that do cut:
AarI AcuI AflIII AhdI AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbvCI BclI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I FalI FspI HaeII Hin4I MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI
PfoI PpuMI PshAI PspOMI PstI PvuII SacI SanDI
SapI SbfI SexAI SfcI SmaI SmlI SphI StuI
StyI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BcgI BciVI BfrBI BglII BmtI BsaBI
BsaWI BsaXI BsiEI BsiWI BsmI BspEI BspHI BsrDI
BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI DraI
EagI EcoNI EcoRI EcoRV FseI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SacII SalI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI TatI Tth111I XbaI XhoI ZraI