Restriction map for
pFN21AE0984
(Linear) (Six-base) MAPSORT of: KIEE0984.seq Check: 890 from: 1 to: 1776
KIEE0984.orf
With 182 enzymes: SgfI *
March 22, 2012 15:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1660 1776
Size: 1660 116
AleI CACnn'nnGTG
Cuts at: 0 1355 1776
Size: 1355 421
AlwNI CAG_nnn'CTG
Cuts at: 0 457 1776
Size: 457 1319
ApoI r'AATT_y
Cuts at: 0 221 238 880 1536 1561 1746 1776
Size: 221 17 642 656 25 185 30
Fragments arranged by size:
656 642 221 185 30 25 17
AseI AT'TA_AT
Cuts at: 0 934 1776
Size: 934 842
BamHI G'GATC_C
Cuts at: 0 1440 1776
Size: 1440 336
BanII G_rGCy'C
Cuts at: 0 504 694 1776
Size: 504 190 1082
Fragments arranged by size:
1082 504 190
BciVI GTATCCnnnnn_n'
Cuts at: 0 408 1776
Size: 408 1368
BclI T'GATC_A
Cuts at: 0 569 1776
Size: 569 1207
BglII A'GATC_T
Cuts at: 0 1546 1776
Size: 1546 230
BmrI ACTGGGnnnn_n'
Cuts at: 0 86 396 1349 1776
Size: 86 310 953 427
Fragments arranged by size:
953 427 310 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 1776
Size: 1169 607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 1776
Size: 704 1072
BsaI GGTCTCn'nnnn_
Cuts at: 0 105 1776
Size: 105 1671
BsaBI GATnn'nnATC
Cuts at: 0 1700 1776
Size: 1700 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1265 1295 1776
Size: 1265 30 481
Fragments arranged by size:
1265 481 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1115 1776
Size: 1115 661
BseYI C'CCAG_C
Cuts at: 0 703 1231 1776
Size: 703 528 545
Fragments arranged by size:
703 545 528
BsiHKAI G_wGCw'C
Cuts at: 0 504 1776
Size: 504 1272
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1380 1776
Size: 1380 396
Bsp1286I G_dGCh'C
Cuts at: 0 504 694 1776
Size: 504 190 1082
Fragments arranged by size:
1082 504 190
BspHI T'CATG_A
Cuts at: 0 53 846 1776
Size: 53 793 930
Fragments arranged by size:
930 793 53
BsrDI GCAATG_nn'
Cuts at: 0 1266 1731 1776
Size: 1266 465 45
Fragments arranged by size:
1266 465 45
BstYI r'GATC_y
Cuts at: 0 323 1440 1546 1776
Size: 323 1117 106 230
Fragments arranged by size:
1117 323 230 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 154 1776
Size: 154 1622
BtsI GCAGTG_nn'
Cuts at: 0 1006 1776
Size: 1006 770
EaeI y'GGCC_r
Cuts at: 0 89 482 581 1728 1776
Size: 89 393 99 1147 48
Fragments arranged by size:
1147 393 99 89 48
EarI CTCTTCn'nnn_
Cuts at: 0 322 1092 1636 1747 1776
Size: 322 770 544 111 29
Fragments arranged by size:
770 544 322 111 29
EcoICRI GAG'CTC
Cuts at: 0 502 1776
Size: 502 1274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 1660 1776
Size: 1169 491 116
Fragments arranged by size:
1169 491 116
EcoO109I rG'GnC_Cy
Cuts at: 0 1447 1776
Size: 1447 329
EcoRI G'AATT_C
Cuts at: 0 1561 1776
Size: 1561 215
EcoRV GAT'ATC
Cuts at: 0 549 824 1107 1776
Size: 549 275 283 669
Fragments arranged by size:
669 549 283 275
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1159 1776
Size: 1159 617
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 808 840 1091 1123 1776
Size: 808 32 251 32 653
Fragments arranged by size:
808 653 251 32 32
HincII GTy'rAC
Cuts at: 0 129 1150 1367 1776
Size: 129 1021 217 409
Fragments arranged by size:
1021 409 217 129
HindIII A'AGCT_T
Cuts at: 0 760 1776
Size: 760 1016
HpaI GTT'AAC
Cuts at: 0 1367 1776
Size: 1367 409
MfeI C'AATT_G
Cuts at: 0 335 1776
Size: 335 1441
MscI TGG'CCA
Cuts at: 0 91 484 583 1730 1776
Size: 91 393 99 1147 46
Fragments arranged by size:
1147 393 99 91 46
MslI CAynn'nnrTG
Cuts at: 0 577 1355 1776
Size: 577 778 421
Fragments arranged by size:
778 577 421
PflMI CCAn_nnn'nTGG
Cuts at: 0 491 1776
Size: 491 1285
PpuMI rG'GwC_Cy
Cuts at: 0 1447 1776
Size: 1447 329
PstI C_TGCA'G
Cuts at: 0 914 1776
Size: 914 862
SacI G_AGCT'C
Cuts at: 0 504 1776
Size: 504 1272
SexAI A'CCwGG_T
Cuts at: 0 182 1776
Size: 182 1594
SfcI C'TryA_G
Cuts at: 0 910 1776
Size: 910 866
SmlI C'TyrA_G
Cuts at: 0 719 1776
Size: 719 1057
SspI AAT'ATT
Cuts at: 0 390 1776
Size: 390 1386
StyI C'CwwG_G
Cuts at: 0 18 1058 1776
Size: 18 1040 718
Fragments arranged by size:
1040 718 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1308 1776
Size: 1308 468
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 527 559 576 608 1265 1297 1776
Size: 527 32 17 32 657 32 479
Fragments arranged by size:
657 527 479 32 32 32 17
Tth111I GACn'n_nGTC
Cuts at: 0 1145 1776
Size: 1145 631
XmnI GAAnn'nnTTC
Cuts at: 0 1169 1561 1776
Size: 1169 392 215
Fragments arranged by size:
1169 392 215
Enzymes that do cut:
AcuI AleI AlwNI ApoI AseI BamHI BanII BciVI
BclI BglII BmrI BpmI BpuEI BsaI BsaBI BsaXI
BseRI BseYI BsiHKAI BsmBI Bsp1286I BspHI BsrDI BstYI
BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI Hin4I HincII HindIII HpaI MfeI MscI
MslI PflMI PpuMI PstI SacI SexAI SfcI SmlI
SspI StyI TaqII TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BanI BbeI BbsI BbvCI BcgI
BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI Bpu10I
BsaAI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI FseI FspI FspAI
HaeII KasI KpnI MluI MmeI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TatI XbaI XcmI XhoI XmaI ZraI