Restriction map for
pFN21AE0978
(Linear) (Six-base) MAPSORT of: KIEE0978.seq Check: 6339 from: 1 to: 1647
KIEE0978.orf
With 182 enzymes: SgfI *
January 29, 2014 11:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 359 692 983 1152 1647
Size: 359 333 291 169 495
Fragments arranged by size:
495 359 333 291 169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 1448 1647
Size: 926 522 199
Fragments arranged by size:
926 522 199
AfeI AGC'GCT
Cuts at: 0 1196 1647
Size: 1196 451
AflIII A'CryG_T
Cuts at: 0 80 261 1647
Size: 80 181 1386
Fragments arranged by size:
1386 181 80
AlwNI CAG_nnn'CTG
Cuts at: 0 360 1015 1647
Size: 360 655 632
Fragments arranged by size:
655 632 360
ApaI G_GGCC'C
Cuts at: 0 1022 1647
Size: 1022 625
AvaI C'yCGr_G
Cuts at: 0 1025 1243 1498 1647
Size: 1025 218 255 149
Fragments arranged by size:
1025 255 218 149
AvrII C'CTAG_G
Cuts at: 0 841 1647
Size: 841 806
BanI G'GyrC_C
Cuts at: 0 542 1562 1647
Size: 542 1020 85
Fragments arranged by size:
1020 542 85
BanII G_rGCy'C
Cuts at: 0 433 719 1022 1034 1316 1647
Size: 433 286 303 12 282 331
Fragments arranged by size:
433 331 303 286 282 12
BbeI G_GCGC'C
Cuts at: 0 1566 1647
Size: 1566 81
BbvCI CC'TCA_GC
Cuts at: 0 448 1647
Size: 448 1199
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 447 481 1647
Size: 447 34 1166
Fragments arranged by size:
1166 447 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1503 1560 1647
Size: 1503 57 87
Fragments arranged by size:
1503 87 57
BglII A'GATC_T
Cuts at: 0 335 1647
Size: 335 1312
BlpI GC'TnA_GC
Cuts at: 0 364 427 718 752 1647
Size: 364 63 291 34 895
Fragments arranged by size:
895 364 291 63 34
Bme1580I G_kGCm'C
Cuts at: 0 1022 1647
Size: 1022 625
BmgBI CAC'GTC
Cuts at: 0 243 286 1647
Size: 243 43 1361
Fragments arranged by size:
1361 243 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 1311 1647
Size: 1311 336
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 72 688 1647
Size: 72 616 959
Fragments arranged by size:
959 616 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 109 202 670 853 1164 1219 1474
Size: 61 48 93 468 183 311 55 255
Cuts at: 1474 1647
Size: 173
Fragments arranged by size:
468 311 255 183 173 93 61 55
48
Bpu10I CC'TnA_GC
Cuts at: 0 47 448 610 1647
Size: 47 401 162 1037
Fragments arranged by size:
1037 401 162 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1582 1647
Size: 1582 65
BsaI GGTCTCn'nnnn_
Cuts at: 0 1418 1647
Size: 1418 229
BsaAI yAC'GTr
Cuts at: 0 81 1647
Size: 81 1566
BsaHI Gr'CG_yC
Cuts at: 0 1563 1647
Size: 1563 84
BsaWI w'CCGG_w
Cuts at: 0 508 1647
Size: 508 1139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 673 703 1303 1333 1647
Size: 673 30 600 30 314
Fragments arranged by size:
673 600 314 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 933 1171 1647
Size: 346 587 238 476
Fragments arranged by size:
587 476 346 238
BseYI C'CCAG_C
Cuts at: 0 28 382 783 868 1015 1484 1515 1647
Size: 28 354 401 85 147 469 31 132
Fragments arranged by size:
469 401 354 147 132 85 31 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 628 961 1192 1647
Size: 505 123 333 231 455
Fragments arranged by size:
505 455 333 231 123
BsiEI CG_ry'CG
Cuts at: 0 1122 1647
Size: 1122 525
BsiHKAI G_wGCw'C
Cuts at: 0 433 1647
Size: 433 1214
Bsp1286I G_dGCh'C
Cuts at: 0 433 719 1022 1034 1316 1647
Size: 433 286 303 12 282 331
Fragments arranged by size:
433 331 303 286 282 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 326 359 692 983 1152 1258 1259 1647
Size: 326 33 333 291 169 106 1 388
Fragments arranged by size:
388 333 326 291 169 106 33 1
BsrBI CCG'CTC
Cuts at: 0 564 1647
Size: 564 1083
BsrFI r'CCGG_y
Cuts at: 0 1054 1117 1647
Size: 1054 63 530
Fragments arranged by size:
1054 530 63
BssSI C'ACGA_G
Cuts at: 0 1210 1647
Size: 1210 437
BstAPI GCAn_nnn'nTGC
Cuts at: 0 360 1221 1647
Size: 360 861 426
Fragments arranged by size:
861 426 360
BstXI CCAn_nnnn'nTGG
Cuts at: 0 811 1647
Size: 811 836
BstYI r'GATC_y
Cuts at: 0 167 335 1647
Size: 167 168 1312
Fragments arranged by size:
1312 168 167
Bsu36I CC'TnA_GG
Cuts at: 0 403 798 1046 1647
Size: 403 395 248 601
Fragments arranged by size:
601 403 395 248
BtgI C'CryG_G
Cuts at: 0 252 437 1334 1647
Size: 252 185 897 313
Fragments arranged by size:
897 313 252 185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 203 1647
Size: 203 1444
BtsI GCAGTG_nn'
Cuts at: 0 375 1177 1647
Size: 375 802 470
Fragments arranged by size:
802 470 375
DraIII CAC_nnn'GTG
Cuts at: 0 552 1647
Size: 552 1095
EaeI y'GGCC_r
Cuts at: 0 158 813 969 999 1077 1119 1293 1647
Size: 158 655 156 30 78 42 174 354
Fragments arranged by size:
655 354 174 158 156 78 42 30
EagI C'GGCC_G
Cuts at: 0 1119 1647
Size: 1119 528
EarI CTCTTCn'nnn_
Cuts at: 0 523 1647
Size: 523 1124
EcoICRI GAG'CTC
Cuts at: 0 431 1647
Size: 431 1216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 109 202 670 853 926 1164 1219
Size: 61 48 93 468 183 73 238 55
Cuts at: 1219 1448 1474 1647
Size: 229 26 173
Fragments arranged by size:
468 238 229 183 173 93 73 61
55 48 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 955 1261 1647
Size: 955 306 386
Fragments arranged by size:
955 386 306
EcoO109I rG'GnC_Cy
Cuts at: 0 498 515 876 960 1019 1233 1647
Size: 498 17 361 84 59 214 414
Fragments arranged by size:
498 414 361 214 84 59 17
FseI GG_CCGG'CC
Cuts at: 0 1058 1121 1647
Size: 1058 63 526
Fragments arranged by size:
1058 526 63
HaeII r_GCGC'y
Cuts at: 0 1198 1207 1355 1566 1647
Size: 1198 9 148 211 81
Fragments arranged by size:
1198 211 148 81 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 735 767 865 897 1647
Size: 735 32 98 32 750
Fragments arranged by size:
750 735 98 32 32
HincII GTy'rAC
Cuts at: 0 267 1647
Size: 267 1380
KasI G'GCGC_C
Cuts at: 0 1562 1647
Size: 1562 85
MscI TGG'CCA
Cuts at: 0 815 971 1001 1295 1647
Size: 815 156 30 294 352
Fragments arranged by size:
815 352 294 156 30
MspA1I CmG'CkG
Cuts at: 0 357 447 489 1494 1519 1647
Size: 357 90 42 1005 25 128
Fragments arranged by size:
1005 357 128 90 42 25
NaeI GCC'GGC
Cuts at: 0 1056 1119 1647
Size: 1056 63 528
Fragments arranged by size:
1056 528 63
NarI GG'CG_CC
Cuts at: 0 1563 1647
Size: 1563 84
NgoMIV G'CCGG_C
Cuts at: 0 1054 1117 1647
Size: 1054 63 530
Fragments arranged by size:
1054 530 63
NspI r_CATG'y
Cuts at: 0 658 1647
Size: 658 989
PflMI CCAn_nnn'nTGG
Cuts at: 0 1628 1647
Size: 1628 19
PfoI T'CCnGG_A
Cuts at: 0 494 1256 1647
Size: 494 762 391
Fragments arranged by size:
762 494 391
PmlI CAC'GTG
Cuts at: 0 81 1647
Size: 81 1566
PpuMI rG'GwC_Cy
Cuts at: 0 498 515 1233 1647
Size: 498 17 718 414
Fragments arranged by size:
718 498 414 17
PspOMI G'GGCC_C
Cuts at: 0 1018 1647
Size: 1018 629
PstI C_TGCA'G
Cuts at: 0 356 460 1547 1647
Size: 356 104 1087 100
Fragments arranged by size:
1087 356 104 100
PvuII CAG'CTG
Cuts at: 0 447 489 1494 1647
Size: 447 42 1005 153
Fragments arranged by size:
1005 447 153 42
SacI G_AGCT'C
Cuts at: 0 433 1647
Size: 433 1214
SapI GCTCTTCn'nnn_
Cuts at: 0 523 1647
Size: 523 1124
SfcI C'TryA_G
Cuts at: 0 352 456 1347 1543 1647
Size: 352 104 891 196 104
Fragments arranged by size:
891 352 196 104 104
SfoI GGC'GCC
Cuts at: 0 1564 1647
Size: 1564 83
SmaI CCC'GGG
Cuts at: 0 1245 1647
Size: 1245 402
SmlI C'TyrA_G
Cuts at: 0 1597 1647
Size: 1597 50
StyI C'CwwG_G
Cuts at: 0 841 1647
Size: 841 806
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1354 1498 1647
Size: 1354 144 149
Fragments arranged by size:
1354 149 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 572 604 673 705 1026 1058 1303 1335
Size: 572 32 69 32 321 32 245 32
Cuts at: 1335 1647
Size: 312
Fragments arranged by size:
572 321 312 245 69 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 36 229 546 1647
Size: 36 193 317 1101
Fragments arranged by size:
1101 317 193 36
XmaI C'CCGG_G
Cuts at: 0 1243 1647
Size: 1243 404
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApaI AvaI AvrII
BanI BanII BbeI BbvCI BcgI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I
HincII KasI MscI MspA1I NaeI NarI NgoMIV NspI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI
SapI SfcI SfoI SmaI SmlI StyI TaqII TstI
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI BaeI
BamHI BbsI BciVI BclI BfrBI BmtI BsaBI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstBI
BstEII BstZ17I ClaI CspCI DraI DrdI EciI EcoRI
EcoRV FalI FspI FspAI HindIII HpaI KpnI MfeI
MluI MmeI MslI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI
XmnI ZraI