Restriction map for
pFN21AE0974
(Linear) (Six-base) MAPSORT of: KIEE0974.seq Check: 4192 from: 1 to: 1581
KIEE0974.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 25 77 1581
Size: 25 52 1504
Fragments arranged by size:
1504 52 25
AccI GT'mk_AC
Cuts at: 0 195 348 1581
Size: 195 153 1233
Fragments arranged by size:
1233 195 153
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 430 749 919 1581
Size: 263 167 319 170 662
Fragments arranged by size:
662 319 263 170 167
AflIII A'CryG_T
Cuts at: 0 22 1398 1581
Size: 22 1376 183
Fragments arranged by size:
1376 183 22
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1050 1082 1581
Size: 1050 32 499
Fragments arranged by size:
1050 499 32
AlwNI CAG_nnn'CTG
Cuts at: 0 344 360 756 1581
Size: 344 16 396 825
Fragments arranged by size:
825 396 344 16
ApaI G_GGCC'C
Cuts at: 0 1563 1581
Size: 1563 18
ApaLI G'TGCA_C
Cuts at: 0 1075 1581
Size: 1075 506
AvaI C'yCGr_G
Cuts at: 0 155 1502 1581
Size: 155 1347 79
Fragments arranged by size:
1347 155 79
BanI G'GyrC_C
Cuts at: 0 448 526 904 1511 1581
Size: 448 78 378 607 70
Fragments arranged by size:
607 448 378 78 70
BanII G_rGCy'C
Cuts at: 0 961 1135 1563 1581
Size: 961 174 428 18
Fragments arranged by size:
961 428 174 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 305 1581
Size: 305 1276
BclI T'GATC_A
Cuts at: 0 743 1581
Size: 743 838
BfrBI ATG'CAT
Cuts at: 0 846 1581
Size: 846 735
BglI GCCn_nnn'nGGC
Cuts at: 0 997 1581
Size: 997 584
Bme1580I G_kGCm'C
Cuts at: 0 1079 1563 1581
Size: 1079 484 18
Fragments arranged by size:
1079 484 18
BmgBI CAC'GTC
Cuts at: 0 1401 1581
Size: 1401 180
BmrI ACTGGGnnnn_n'
Cuts at: 0 1446 1581
Size: 1446 135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 704 915 1581
Size: 382 322 211 666
Fragments arranged by size:
666 382 322 211
Bpu10I CC'TnA_GC
Cuts at: 0 1554 1581
Size: 1554 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 784 1581
Size: 576 208 797
Fragments arranged by size:
797 576 208
BsaI GGTCTCn'nnnn_
Cuts at: 0 1492 1581
Size: 1492 89
BsaAI yAC'GTr
Cuts at: 0 1407 1581
Size: 1407 174
BsaBI GATnn'nnATC
Cuts at: 0 650 1581
Size: 650 931
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 708 738 1581
Size: 708 30 843
Fragments arranged by size:
843 708 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 1581
Size: 67 1514
BseYI C'CCAG_C
Cuts at: 0 768 1581
Size: 768 813
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 1581
Size: 3 1578
BsiHKAI G_wGCw'C
Cuts at: 0 961 1079 1581
Size: 961 118 502
Fragments arranged by size:
961 502 118
Bsp1286I G_dGCh'C
Cuts at: 0 961 1079 1135 1563 1581
Size: 961 118 56 428 18
Fragments arranged by size:
961 428 118 56 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 25 77 431 1581
Size: 25 52 354 1150
Fragments arranged by size:
1150 354 52 25
BsrFI r'CCGG_y
Cuts at: 0 280 991 1581
Size: 280 711 590
Fragments arranged by size:
711 590 280
BssSI C'ACGA_G
Cuts at: 0 262 1309 1581
Size: 262 1047 272
Fragments arranged by size:
1047 272 262
BstEII G'GTnAC_C
Cuts at: 0 1303 1581
Size: 1303 278
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1411 1581
Size: 1411 170
BstYI r'GATC_y
Cuts at: 0 564 1581
Size: 564 1017
BstZ17I GTA'TAC
Cuts at: 0 349 1581
Size: 349 1232
Bsu36I CC'TnA_GG
Cuts at: 0 548 1292 1581
Size: 548 744 289
Fragments arranged by size:
744 548 289
BtgI C'CryG_G
Cuts at: 0 808 827 1581
Size: 808 19 754
Fragments arranged by size:
808 754 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1416 1581
Size: 1416 165
BtsI GCAGTG_nn'
Cuts at: 0 1548 1581
Size: 1548 33
EaeI y'GGCC_r
Cuts at: 0 2 308 998 1581
Size: 2 306 690 583
Fragments arranged by size:
690 583 306 2
EarI CTCTTCn'nnn_
Cuts at: 0 52 1490 1581
Size: 52 1438 91
Fragments arranged by size:
1438 91 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 951 1432 1581
Size: 951 481 149
Fragments arranged by size:
951 481 149
EcoICRI GAG'CTC
Cuts at: 0 959 1581
Size: 959 622
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 382 430 704 749 915 919 1581
Size: 263 119 48 274 45 166 4 662
Fragments arranged by size:
662 274 263 166 119 48 45 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1312 1581
Size: 1312 269
EcoO109I rG'GnC_Cy
Cuts at: 0 405 1170 1296 1559 1560 1581
Size: 405 765 126 263 1 21
Fragments arranged by size:
765 405 263 126 21 1
EcoRV GAT'ATC
Cuts at: 0 588 1581
Size: 588 993
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1356 1581
Size: 1356 225
FspI TGC'GCA
Cuts at: 0 874 1581
Size: 874 707
HaeII r_GCGC'y
Cuts at: 0 989 1581
Size: 989 592
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 549 581 685 717 834 866 1251 1283
Size: 549 32 104 32 117 32 385 32
Cuts at: 1283 1581
Size: 298
Fragments arranged by size:
549 385 298 117 104 32 32 32
32
HincII GTy'rAC
Cuts at: 0 196 372 820 1581
Size: 196 176 448 761
Fragments arranged by size:
761 448 196 176
HpaI GTT'AAC
Cuts at: 0 372 1581
Size: 372 1209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1016 1581
Size: 1016 565
MscI TGG'CCA
Cuts at: 0 4 310 1581
Size: 4 306 1271
Fragments arranged by size:
1271 306 4
MslI CAynn'nnrTG
Cuts at: 0 386 708 919 1581
Size: 386 322 211 662
Fragments arranged by size:
662 386 322 211
MspA1I CmG'CkG
Cuts at: 0 1215 1575 1581
Size: 1215 360 6
Fragments arranged by size:
1215 360 6
NaeI GCC'GGC
Cuts at: 0 993 1581
Size: 993 588
NgoMIV G'CCGG_C
Cuts at: 0 991 1581
Size: 991 590
NsiI A_TGCA'T
Cuts at: 0 848 1581
Size: 848 733
PfoI T'CCnGG_A
Cuts at: 0 931 1581
Size: 931 650
PmlI CAC'GTG
Cuts at: 0 1407 1581
Size: 1407 174
PpuMI rG'GwC_Cy
Cuts at: 0 405 1170 1581
Size: 405 765 411
Fragments arranged by size:
765 411 405
PshAI GACnn'nnGTC
Cuts at: 0 1504 1581
Size: 1504 77
PspOMI G'GGCC_C
Cuts at: 0 1559 1581
Size: 1559 22
PstI C_TGCA'G
Cuts at: 0 1580 1581
Size: 1580 1
PvuII CAG'CTG
Cuts at: 0 1215 1581
Size: 1215 366
SacI G_AGCT'C
Cuts at: 0 961 1581
Size: 961 620
SalI G'TCGA_C
Cuts at: 0 194 1581
Size: 194 1387
SapI GCTCTTCn'nnn_
Cuts at: 0 52 1581
Size: 52 1529
ScaI AGT'ACT
Cuts at: 0 319 1581
Size: 319 1262
SfcI C'TryA_G
Cuts at: 0 321 1115 1576 1581
Size: 321 794 461 5
Fragments arranged by size:
794 461 321 5
SmaI CCC'GGG
Cuts at: 0 157 1504 1581
Size: 157 1347 77
Fragments arranged by size:
1347 157 77
SmlI C'TyrA_G
Cuts at: 0 555 799 1581
Size: 555 244 782
Fragments arranged by size:
782 555 244
StuI AGG'CCT
Cuts at: 0 1249 1274 1581
Size: 1249 25 307
Fragments arranged by size:
1249 307 25
StyI C'CwwG_G
Cuts at: 0 1299 1581
Size: 1299 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1465 1581
Size: 1465 116
TatI w'GTAC_w
Cuts at: 0 317 489 1454 1581
Size: 317 172 965 127
Fragments arranged by size:
965 317 172 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 566 598 708 740 1411 1443 1513 1545
Size: 566 32 110 32 671 32 70 32
Cuts at: 1545 1581
Size: 36
Fragments arranged by size:
671 566 110 70 36 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 394 1581
Size: 394 1187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 707 1581
Size: 707 874
XmaI C'CCGG_G
Cuts at: 0 155 1502 1581
Size: 155 1347 79
Fragments arranged by size:
1347 155 79
XmnI GAAnn'nnTTC
Cuts at: 0 251 1581
Size: 251 1330
Enzymes that do cut:
AarI AccI AcuI AflIII AloI AlwNI ApaI ApaLI
AvaI BanI BanII BbsI BclI BfrBI BglI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI
BssSI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI FspI HaeII Hin4I HincII HpaI MmeI
MscI MslI MspA1I NaeI NgoMIV NsiI PfoI PmlI
PpuMI PshAI PspOMI PstI PvuII SacI SalI SapI
ScaI SfcI SmaI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BglII BlpI BmtI BplI
BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BstAPI BstBI ClaI CspCI
DraI DraIII DrdI EagI EcoRI FseI FspAI HindIII
KasI KpnI MfeI MluI NarI NcoI NdeI NheI
NotI NruI NspI PacI PasI PciI PflMI PmeI
PpiI PsiI PspXI PsrI PvuI RsrII SacII SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XhoI ZraI