Restriction map for
pFN21AE0972
(Linear) (Six-base) MAPSORT of: KIEE0972.seq Check: 666 from: 1 to: 1560
KIEE0972.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AatII G_ACGT'C
Cuts at: 0 890 1560
Size: 890 670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 628 1083 1294 1560
Size: 514 114 455 211 266
Fragments arranged by size:
514 455 266 211 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 378 1560
Size: 378 1182
AlwNI CAG_nnn'CTG
Cuts at: 0 570 1560
Size: 570 990
ApaI G_GGCC'C
Cuts at: 0 824 1560
Size: 824 736
AvaI C'yCGr_G
Cuts at: 0 35 302 525 901 1069 1294 1560
Size: 35 267 223 376 168 225 266
Fragments arranged by size:
376 267 266 225 223 168 35
BamHI G'GATC_C
Cuts at: 0 30 775 1560
Size: 30 745 785
Fragments arranged by size:
785 745 30
BanI G'GyrC_C
Cuts at: 0 409 1311 1560
Size: 409 902 249
Fragments arranged by size:
902 409 249
BanII G_rGCy'C
Cuts at: 0 824 1220 1259 1361 1508 1560
Size: 824 396 39 102 147 52
Fragments arranged by size:
824 396 147 102 52 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 455 1560
Size: 455 1105
BbvCI CC'TCA_GC
Cuts at: 0 665 1474 1560
Size: 665 809 86
Fragments arranged by size:
809 665 86
BglI GCCn_nnn'nGGC
Cuts at: 0 1072 1560
Size: 1072 488
BlpI GC'TnA_GC
Cuts at: 0 1028 1560
Size: 1028 532
Bme1580I G_kGCm'C
Cuts at: 0 824 839 977 1168 1560
Size: 824 15 138 191 392
Fragments arranged by size:
824 392 191 138 15
BmgBI CAC'GTC
Cuts at: 0 514 1560
Size: 514 1046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 598 1526 1560
Size: 271 327 928 34
Fragments arranged by size:
928 327 271 34
Bpu10I CC'TnA_GC
Cuts at: 0 665 1474 1560
Size: 665 809 86
Fragments arranged by size:
809 665 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1243 1560
Size: 1243 317
BsaAI yAC'GTr
Cuts at: 0 339 624 782 978 1560
Size: 339 285 158 196 582
Fragments arranged by size:
582 339 285 196 158
BsaBI GATnn'nnATC
Cuts at: 0 601 1560
Size: 601 959
BsaHI Gr'CG_yC
Cuts at: 0 887 998 1133 1560
Size: 887 111 135 427
Fragments arranged by size:
887 427 135 111
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 571 601 1560
Size: 571 30 959
Fragments arranged by size:
959 571 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 874 1267 1369 1387 1390 1420 1423
Size: 100 774 393 102 18 3 30 3
Cuts at: 1423 1560
Size: 137
Fragments arranged by size:
774 393 137 102 100 30 18 3
3
BseYI C'CCAG_C
Cuts at: 0 186 262 660 1053 1438 1560
Size: 186 76 398 393 385 122
Fragments arranged by size:
398 393 385 186 122 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1560
Size: 388 1172
BsiHKAI G_wGCw'C
Cuts at: 0 1259 1382 1560
Size: 1259 123 178
Fragments arranged by size:
1259 178 123
BsmI GAATG_Cn'
Cuts at: 0 1057 1560
Size: 1057 503
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1291 1560
Size: 1291 269
Bsp1286I G_dGCh'C
Cuts at: 0 824 839 977 1168 1220 1259 1361 1382
Size: 824 15 138 191 52 39 102 21
Cuts at: 1382 1508 1560
Size: 126 52
Fragments arranged by size:
824 191 138 126 102 52 52 39
21 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 887 962 1560
Size: 887 75 598
Fragments arranged by size:
887 598 75
BsrBI CCG'CTC
Cuts at: 0 231 582 1560
Size: 231 351 978
Fragments arranged by size:
978 351 231
BsrDI GCAATG_nn'
Cuts at: 0 470 1560
Size: 470 1090
BssHII G'CGCG_C
Cuts at: 0 790 1560
Size: 790 770
BstAPI GCAn_nnn'nTGC
Cuts at: 0 570 1560
Size: 570 990
BstEII G'GTnAC_C
Cuts at: 0 1338 1560
Size: 1338 222
BstYI r'GATC_y
Cuts at: 0 30 775 1560
Size: 30 745 785
Fragments arranged by size:
785 745 30
Bsu36I CC'TnA_GG
Cuts at: 0 1232 1560
Size: 1232 328
BtgI C'CryG_G
Cuts at: 0 136 275 1220 1448 1560
Size: 136 139 945 228 112
Fragments arranged by size:
945 228 139 136 112
BtsI GCAGTG_nn'
Cuts at: 0 912 1380 1560
Size: 912 468 180
Fragments arranged by size:
912 468 180
ClaI AT'CG_AT
Cuts at: 0 68 1560
Size: 68 1492
DraIII CAC_nnn'GTG
Cuts at: 0 339 1560
Size: 339 1221
DrdI GACnn_nn'nnGTC
Cuts at: 0 997 1560
Size: 997 563
EaeI y'GGCC_r
Cuts at: 0 232 341 1089 1560
Size: 232 109 748 471
Fragments arranged by size:
748 471 232 109
EarI CTCTTCn'nnn_
Cuts at: 0 1517 1560
Size: 1517 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 327 1560
Size: 327 1233
EcoICRI GAG'CTC
Cuts at: 0 1257 1560
Size: 1257 303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 514 598 628 1083 1294 1526 1560
Size: 271 243 84 30 455 211 232 34
Fragments arranged by size:
455 271 243 232 211 84 34 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 312 814 1560
Size: 312 502 746
Fragments arranged by size:
746 502 312
EcoO109I rG'GnC_Cy
Cuts at: 0 280 533 648 820 1263 1560
Size: 280 253 115 172 443 297
Fragments arranged by size:
443 297 280 253 172 115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1503 1560
Size: 1503 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 760 792 1560
Size: 760 32 768
Fragments arranged by size:
768 760 32
HindIII A'AGCT_T
Cuts at: 0 214 1560
Size: 214 1346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 1560
Size: 974 586
MscI TGG'CCA
Cuts at: 0 343 1560
Size: 343 1217
MslI CAynn'nnrTG
Cuts at: 0 70 626 832 1560
Size: 70 556 206 728
Fragments arranged by size:
728 556 206 70
MspA1I CmG'CkG
Cuts at: 0 16 372 567 664 1032 1442 1560
Size: 16 356 195 97 368 410 118
Fragments arranged by size:
410 368 356 195 118 97 16
PasI CC'CwG_GG
Cuts at: 0 546 1560
Size: 546 1014
PflMI CCAn_nnn'nTGG
Cuts at: 0 339 1560
Size: 339 1221
PfoI T'CCnGG_A
Cuts at: 0 650 1542 1560
Size: 650 892 18
Fragments arranged by size:
892 650 18
PmlI CAC'GTG
Cuts at: 0 339 624 782 978 1560
Size: 339 285 158 196 582
Fragments arranged by size:
582 339 285 196 158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1467 1499 1560
Size: 1467 32 61
Fragments arranged by size:
1467 61 32
PpuMI rG'GwC_Cy
Cuts at: 0 533 648 1263 1560
Size: 533 115 615 297
Fragments arranged by size:
615 533 297 115
PspOMI G'GGCC_C
Cuts at: 0 820 1560
Size: 820 740
PspXI vC'TCGA_Gb
Cuts at: 0 901 1560
Size: 901 659
PstI C_TGCA'G
Cuts at: 0 392 692 1387 1560
Size: 392 300 695 173
Fragments arranged by size:
695 392 300 173
PvuII CAG'CTG
Cuts at: 0 16 372 567 664 1032 1442 1560
Size: 16 356 195 97 368 410 118
Fragments arranged by size:
410 368 356 195 118 97 16
SacI G_AGCT'C
Cuts at: 0 1259 1560
Size: 1259 301
SanDI GG'GwC_CC
Cuts at: 0 533 1560
Size: 533 1027
SapI GCTCTTCn'nnn_
Cuts at: 0 1517 1560
Size: 1517 43
ScaI AGT'ACT
Cuts at: 0 171 907 1560
Size: 171 736 653
Fragments arranged by size:
736 653 171
SfcI C'TryA_G
Cuts at: 0 223 388 688 1383 1560
Size: 223 165 300 695 177
Fragments arranged by size:
695 300 223 177 165
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1072 1560
Size: 1072 488
SmaI CCC'GGG
Cuts at: 0 304 1560
Size: 304 1256
SmlI C'TyrA_G
Cuts at: 0 901 1258 1560
Size: 901 357 302
Fragments arranged by size:
901 357 302
StuI AGG'CCT
Cuts at: 0 1111 1560
Size: 1111 449
StyI C'CwwG_G
Cuts at: 0 1112 1333 1560
Size: 1112 221 227
Fragments arranged by size:
1112 227 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1326 1560
Size: 1326 234
TatI w'GTAC_w
Cuts at: 0 169 380 905 1142 1560
Size: 169 211 525 237 418
Fragments arranged by size:
525 418 237 211 169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 957 1099 1455 1560
Size: 957 142 356 105
Fragments arranged by size:
957 356 142 105
XhoI C'TCGA_G
Cuts at: 0 901 1560
Size: 901 659
XmaI C'CCGG_G
Cuts at: 0 302 1560
Size: 302 1258
ZraI GAC'GTC
Cuts at: 0 888 1560
Size: 888 672
Enzymes that do cut:
AatII AcuI AlfI AlwNI ApaI AvaI BamHI BanI
BanII BbsI BbvCI BglI BlpI Bme1580I BmgBI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BssHII BstAPI BstEII BstYI Bsu36I BtgI BtsI ClaI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI Hin4I HindIII MmeI MscI MslI MspA1I
PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI
PstI PvuII SacI SanDI SapI ScaI SfcI SfiI
SmaI SmlI StuI StyI TaqII TatI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglII
BmrI BmtI BplI BsaI BsaWI BsiEI BsiWI BspEI
BspHI BsrFI BsrGI BssSI BstBI BstXI BstZ17I BtgZI
CspCI DraI EagI EcoRI EcoRV FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PshAI PsiI PsrI PvuI
RsrII SacII SalI SbfI SexAI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XmnI