Restriction map for
pFN21AE0962
(Linear) (Six-base) MAPSORT of: KIEE0962.seq Check: 9672 from: 1 to: 1509
KIEE0962.orf
With 182 enzymes: SgfI *
March 22, 2012 15:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 944 1509
Size: 944 565
Acc65I G'GTAC_C
Cuts at: 0 1415 1509
Size: 1415 94
AflIII A'CryG_T
Cuts at: 0 103 293 1509
Size: 103 190 1216
Fragments arranged by size:
1216 190 103
AleI CACnn'nnGTG
Cuts at: 0 157 1509
Size: 157 1352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 175 1509
Size: 175 1334
AlwNI CAG_nnn'CTG
Cuts at: 0 542 879 1509
Size: 542 337 630
Fragments arranged by size:
630 542 337
ApaI G_GGCC'C
Cuts at: 0 88 373 1509
Size: 88 285 1136
Fragments arranged by size:
1136 285 88
ApaLI G'TGCA_C
Cuts at: 0 150 1509
Size: 150 1359
AvaI C'yCGr_G
Cuts at: 0 80 696 804 1101 1132 1509
Size: 80 616 108 297 31 377
Fragments arranged by size:
616 377 297 108 80 31
AvrII C'CTAG_G
Cuts at: 0 768 1509
Size: 768 741
BanI G'GyrC_C
Cuts at: 0 182 236 1111 1128 1284 1415 1509
Size: 182 54 875 17 156 131 94
Fragments arranged by size:
875 182 156 131 94 54 17
BanII G_rGCy'C
Cuts at: 0 13 88 115 227 373 569 887 1509
Size: 13 75 27 112 146 196 318 622
Fragments arranged by size:
622 318 196 146 112 75 27 13
BbeI G_GCGC'C
Cuts at: 0 240 1509
Size: 240 1269
BbsI GAAGACnn'nnnn_
Cuts at: 0 1465 1509
Size: 1465 44
BglI GCCn_nnn'nGGC
Cuts at: 0 388 397 638 1509
Size: 388 9 241 871
Fragments arranged by size:
871 388 241 9
BglII A'GATC_T
Cuts at: 0 686 731 947 1509
Size: 686 45 216 562
Fragments arranged by size:
686 562 216 45
Bme1580I G_kGCm'C
Cuts at: 0 88 154 373 1114 1289 1509
Size: 88 66 219 741 175 220
Fragments arranged by size:
741 220 219 175 88 66
BmtI G_CTAG'C
Cuts at: 0 907 1509
Size: 907 602
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 864 1509
Size: 864 645
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 901 1509
Size: 328 573 608
Fragments arranged by size:
608 573 328
Bpu10I CC'TnA_GC
Cuts at: 0 561 1509
Size: 561 948
BsaI GGTCTCn'nnnn_
Cuts at: 0 56 1509
Size: 56 1453
BsaAI yAC'GTr
Cuts at: 0 104 942 1509
Size: 104 838 567
Fragments arranged by size:
838 567 104
BsaHI Gr'CG_yC
Cuts at: 0 237 1509
Size: 237 1272
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 53 83 306 336 778 808 1399 1429
Size: 53 30 223 30 442 30 591 30
Cuts at: 1429 1509
Size: 80
Fragments arranged by size:
591 442 223 80 53 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 684 1509
Size: 187 497 825
Fragments arranged by size:
825 497 187
BseYI C'CCAG_C
Cuts at: 0 468 1297 1509
Size: 468 829 212
Fragments arranged by size:
829 468 212
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 442 528 601 922 1509
Size: 84 358 86 73 321 587
Fragments arranged by size:
587 358 321 86 84 73
BsiHKAI G_wGCw'C
Cuts at: 0 154 227 811 845 1509
Size: 154 73 584 34 664
Fragments arranged by size:
664 584 154 73 34
BsmBI CGTCTCn'nnnn_
Cuts at: 0 83 1509
Size: 83 1426
Bsp1286I G_dGCh'C
Cuts at: 0 13 88 115 154 227 373 569 811
Size: 13 75 27 39 73 146 196 242
Cuts at: 811 845 887 1114 1289 1509
Size: 34 42 227 175 220
Fragments arranged by size:
242 227 220 196 175 146 75 73
42 39 34 27 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 106 142 591 944 1031 1509
Size: 106 36 449 353 87 478
Fragments arranged by size:
478 449 353 106 87 36
BsrDI GCAATG_nn'
Cuts at: 0 117 974 1008 1509
Size: 117 857 34 501
Fragments arranged by size:
857 501 117 34
BsrFI r'CCGG_y
Cuts at: 0 1358 1509
Size: 1358 151
BssSI C'ACGA_G
Cuts at: 0 838 1509
Size: 838 671
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1163 1509
Size: 1163 346
BstEII G'GTnAC_C
Cuts at: 0 51 93 1509
Size: 51 42 1416
Fragments arranged by size:
1416 51 42
BstYI r'GATC_y
Cuts at: 0 686 731 947 1151 1509
Size: 686 45 216 204 358
Fragments arranged by size:
686 358 216 204 45
BtgI C'CryG_G
Cuts at: 0 914 1509
Size: 914 595
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 762 1020 1509
Size: 762 258 489
Fragments arranged by size:
762 489 258
BtsI GCAGTG_nn'
Cuts at: 0 1033 1509
Size: 1033 476
DraIII CAC_nnn'GTG
Cuts at: 0 294 1509
Size: 294 1215
EaeI y'GGCC_r
Cuts at: 0 348 677 1509
Size: 348 329 832
Fragments arranged by size:
832 348 329
EcoICRI GAG'CTC
Cuts at: 0 225 1509
Size: 225 1284
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 901 1509
Size: 328 573 608
Fragments arranged by size:
608 573 328
EcoO109I rG'GnC_Cy
Cuts at: 0 84 127 143 370 525 1509
Size: 84 43 16 227 155 984
Fragments arranged by size:
984 227 155 84 43 16
HaeII r_GCGC'y
Cuts at: 0 240 1509
Size: 240 1269
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 694 726 778 810 1509
Size: 694 32 52 32 699
Fragments arranged by size:
699 694 52 32 32
HincII GTy'rAC
Cuts at: 0 255 1509
Size: 255 1254
KasI G'GCGC_C
Cuts at: 0 236 1509
Size: 236 1273
KpnI G_GTAC'C
Cuts at: 0 1419 1509
Size: 1419 90
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 1509
Size: 453 1056
MscI TGG'CCA
Cuts at: 0 350 1509
Size: 350 1159
MslI CAynn'nnrTG
Cuts at: 0 157 1509
Size: 157 1352
MspA1I CmG'CkG
Cuts at: 0 90 354 816 916 1509
Size: 90 264 462 100 593
Fragments arranged by size:
593 462 264 100 90
NarI GG'CG_CC
Cuts at: 0 237 1509
Size: 237 1272
NheI G'CTAG_C
Cuts at: 0 903 1509
Size: 903 606
NspI r_CATG'y
Cuts at: 0 427 519 923 1509
Size: 427 92 404 586
Fragments arranged by size:
586 427 404 92
PasI CC'CwG_GG
Cuts at: 0 57 402 1509
Size: 57 345 1107
Fragments arranged by size:
1107 345 57
PfoI T'CCnGG_A
Cuts at: 0 530 719 1509
Size: 530 189 790
Fragments arranged by size:
790 530 189
PmlI CAC'GTG
Cuts at: 0 104 942 1509
Size: 104 838 567
Fragments arranged by size:
838 567 104
PpuMI rG'GwC_Cy
Cuts at: 0 525 1509
Size: 525 984
PspOMI G'GGCC_C
Cuts at: 0 84 369 1509
Size: 84 285 1140
Fragments arranged by size:
1140 285 84
PspXI vC'TCGA_Gb
Cuts at: 0 696 804 1509
Size: 696 108 705
Fragments arranged by size:
705 696 108
PstI C_TGCA'G
Cuts at: 0 232 869 1028 1371 1509
Size: 232 637 159 343 138
Fragments arranged by size:
637 343 232 159 138
PvuII CAG'CTG
Cuts at: 0 354 816 1509
Size: 354 462 693
Fragments arranged by size:
693 462 354
SacI G_AGCT'C
Cuts at: 0 227 1509
Size: 227 1282
SacII CC_GC'GG
Cuts at: 0 917 1509
Size: 917 592
SfcI C'TryA_G
Cuts at: 0 228 865 1024 1260 1367 1509
Size: 228 637 159 236 107 142
Fragments arranged by size:
637 236 228 159 142 107
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 388 397 1509
Size: 388 9 1112
Fragments arranged by size:
1112 388 9
SfoI GGC'GCC
Cuts at: 0 238 1509
Size: 238 1271
SmlI C'TyrA_G
Cuts at: 0 696 804 1509
Size: 696 108 705
Fragments arranged by size:
705 696 108
SphI G_CATG'C
Cuts at: 0 923 1509
Size: 923 586
StuI AGG'CCT
Cuts at: 0 26 1388 1509
Size: 26 1362 121
Fragments arranged by size:
1362 121 26
StyI C'CwwG_G
Cuts at: 0 5 47 608 768 1509
Size: 5 42 561 160 741
Fragments arranged by size:
741 561 160 42 5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 331 394 1509
Size: 331 63 1115
Fragments arranged by size:
1115 331 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 53 85 280 306 312 338 1509
Size: 53 32 195 26 6 26 1171
Fragments arranged by size:
1171 195 53 32 26 26 6
Tth111I GACn'n_nGTC
Cuts at: 0 1375 1509
Size: 1375 134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1122 1509
Size: 1122 387
XhoI C'TCGA_G
Cuts at: 0 696 804 1509
Size: 696 108 705
Fragments arranged by size:
705 696 108
Enzymes that do cut:
AarI Acc65I AflIII AleI AlfI AlwNI ApaI ApaLI
AvaI AvrII BanI BanII BbeI BbsI BglI BglII
Bme1580I BmtI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BsrDI BsrFI BssSI BstAPI BstEII BstYI BtgI BtgZI
BtsI DraIII EaeI EcoICRI Eco57MI EcoO109I HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NarI
NheI NspI PasI PfoI PmlI PpuMI PspOMI PspXI
PstI PvuII SacI SacII SfcI SfiI SfoI SmlI
SphI StuI StyI TaqII TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AgeI AhdI
AloI ApoI AscI AseI AsiSI BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BlpI BmgBI BmrI BpuEI
BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI
BsrGI BssHII BstBI BstXI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EagI EarI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI MfeI MluI
NaeI NcoI NdeI NgoMIV NotI NruI NsiI PacI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PvuI
RsrII SalI SanDI SapI SbfI ScaI SexAI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI
XbaI XmaI XmnI ZraI