Restriction map for
pFN21AE0961
(Linear) (Six-base) MAPSORT of: KIEE0961.seq Check: 9117 from: 1 to: 1503
KIEE0961.orf
With 182 enzymes: SgfI *
December 27, 2011 16:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 160 1042 1503
Size: 160 882 461
Fragments arranged by size:
882 461 160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 1503
Size: 490 1013
AfeI AGC'GCT
Cuts at: 0 1275 1503
Size: 1275 228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1366 1503
Size: 1366 137
AlwNI CAG_nnn'CTG
Cuts at: 0 1033 1096 1192 1240 1264 1288 1503
Size: 1033 63 96 48 24 24 215
Fragments arranged by size:
1033 215 96 63 48 24 24
ApaLI G'TGCA_C
Cuts at: 0 424 1503
Size: 424 1079
AvaI C'yCGr_G
Cuts at: 0 550 1042 1503
Size: 550 492 461
Fragments arranged by size:
550 492 461
BamHI G'GATC_C
Cuts at: 0 329 1503
Size: 329 1174
BanI G'GyrC_C
Cuts at: 0 981 1420 1503
Size: 981 439 83
Fragments arranged by size:
981 439 83
BanII G_rGCy'C
Cuts at: 0 539 1503
Size: 539 964
BbeI G_GCGC'C
Cuts at: 0 1424 1503
Size: 1424 79
BbvCI CC'TCA_GC
Cuts at: 0 1020 1089 1503
Size: 1020 69 414
Fragments arranged by size:
1020 414 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 995 1029 1503
Size: 995 34 474
Fragments arranged by size:
995 474 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1015 1354 1363 1503
Size: 1015 339 9 140
Fragments arranged by size:
1015 339 140 9
BlpI GC'TnA_GC
Cuts at: 0 509 1503
Size: 509 994
Bme1580I G_kGCm'C
Cuts at: 0 428 906 984 1331 1476 1503
Size: 428 478 78 347 145 27
Fragments arranged by size:
478 428 347 145 78 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 1005 1503
Size: 1005 498
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 759 858 1295 1503
Size: 759 99 437 208
Fragments arranged by size:
759 437 208 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 409 463 1217 1265 1503
Size: 316 93 54 754 48 238
Fragments arranged by size:
754 316 238 93 54 48
Bpu10I CC'TnA_GC
Cuts at: 0 1020 1089 1379 1503
Size: 1020 69 290 124
Fragments arranged by size:
1020 290 124 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 400 421 1503
Size: 205 195 21 1082
Fragments arranged by size:
1082 205 195 21
BsaBI GATnn'nnATC
Cuts at: 0 214 487 701 1503
Size: 214 273 214 802
Fragments arranged by size:
802 273 214 214
BsaHI Gr'CG_yC
Cuts at: 0 594 1421 1503
Size: 594 827 82
Fragments arranged by size:
827 594 82
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 679 1503
Size: 112 567 824
Fragments arranged by size:
824 567 112
BseYI C'CCAG_C
Cuts at: 0 608 1161 1185 1209 1257 1394 1503
Size: 608 553 24 24 48 137 109
Fragments arranged by size:
608 553 137 109 48 24 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1503
Size: 130 1373
BsiEI CG_ry'CG
Cuts at: 0 180 1503
Size: 180 1323
BsiHKAI G_wGCw'C
Cuts at: 0 428 1324 1503
Size: 428 896 179
Fragments arranged by size:
896 428 179
Bsp1286I G_dGCh'C
Cuts at: 0 428 539 906 984 1324 1331 1476 1503
Size: 428 111 367 78 340 7 145 27
Fragments arranged by size:
428 367 340 145 111 78 27 7
BspHI T'CATG_A
Cuts at: 0 965 1503
Size: 965 538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 160 1042 1503
Size: 160 882 461
Fragments arranged by size:
882 461 160
BsrDI GCAATG_nn'
Cuts at: 0 870 1503
Size: 870 633
BsrFI r'CCGG_y
Cuts at: 0 464 578 1383 1503
Size: 464 114 805 120
Fragments arranged by size:
805 464 120 114
BsrGI T'GTAC_A
Cuts at: 0 629 1503
Size: 629 874
BssSI C'ACGA_G
Cuts at: 0 1112 1503
Size: 1112 391
BstXI CCAn_nnnn'nTGG
Cuts at: 0 783 1503
Size: 783 720
BstYI r'GATC_y
Cuts at: 0 329 716 1503
Size: 329 387 787
Fragments arranged by size:
787 387 329
Bsu36I CC'TnA_GG
Cuts at: 0 1097 1503
Size: 1097 406
BtgI C'CryG_G
Cuts at: 0 305 395 1503
Size: 305 90 1108
Fragments arranged by size:
1108 305 90
BtsI GCAGTG_nn'
Cuts at: 0 824 1031 1206 1350 1503
Size: 824 207 175 144 153
Fragments arranged by size:
824 207 175 153 144
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 353 388 1503
Size: 353 35 1115
Fragments arranged by size:
1115 353 35
EaeI y'GGCC_r
Cuts at: 0 177 302 1355 1503
Size: 177 125 1053 148
Fragments arranged by size:
1053 177 148 125
EagI C'GGCC_G
Cuts at: 0 177 1503
Size: 177 1326
EarI CTCTTCn'nnn_
Cuts at: 0 712 827 1310 1451 1503
Size: 712 115 483 141 52
Fragments arranged by size:
712 483 141 115 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 409 463 490 1217 1265 1503
Size: 316 93 54 27 727 48 238
Fragments arranged by size:
727 316 238 93 54 48 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 337 529 1503
Size: 337 192 974
Fragments arranged by size:
974 337 192
EcoO109I rG'GnC_Cy
Cuts at: 0 545 1503
Size: 545 958
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 300 723 1503
Size: 300 423 780
Fragments arranged by size:
780 423 300
HaeII r_GCGC'y
Cuts at: 0 1277 1424 1503
Size: 1277 147 79
Fragments arranged by size:
1277 147 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 71 103 1503
Size: 71 32 1400
Fragments arranged by size:
1400 71 32
KasI G'GCGC_C
Cuts at: 0 1420 1503
Size: 1420 83
MscI TGG'CCA
Cuts at: 0 304 1357 1503
Size: 304 1053 146
Fragments arranged by size:
1053 304 146
MslI CAynn'nnrTG
Cuts at: 0 964 1503
Size: 964 539
MspA1I CmG'CkG
Cuts at: 0 252 585 819 1066 1072 1369 1503
Size: 252 333 234 247 6 297 134
Fragments arranged by size:
333 297 252 247 234 134 6
NaeI GCC'GGC
Cuts at: 0 580 1385 1503
Size: 580 805 118
Fragments arranged by size:
805 580 118
NarI GG'CG_CC
Cuts at: 0 1421 1503
Size: 1421 82
NgoMIV G'CCGG_C
Cuts at: 0 578 1383 1503
Size: 578 805 120
Fragments arranged by size:
805 578 120
PfoI T'CCnGG_A
Cuts at: 0 293 332 386 1503
Size: 293 39 54 1117
Fragments arranged by size:
1117 293 54 39
PpuMI rG'GwC_Cy
Cuts at: 0 545 1503
Size: 545 958
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 439 471 1503
Size: 439 32 1032
Fragments arranged by size:
1032 439 32
PstI C_TGCA'G
Cuts at: 0 831 1008 1053 1071 1503
Size: 831 177 45 18 432
Fragments arranged by size:
831 432 177 45 18
PvuII CAG'CTG
Cuts at: 0 252 1066 1072 1369 1503
Size: 252 814 6 297 134
Fragments arranged by size:
814 297 252 134 6
SanDI GG'GwC_CC
Cuts at: 0 545 1503
Size: 545 958
ScaI AGT'ACT
Cuts at: 0 283 1503
Size: 283 1220
SexAI A'CCwGG_T
Cuts at: 0 449 782 1503
Size: 449 333 721
Fragments arranged by size:
721 449 333
SfcI C'TryA_G
Cuts at: 0 247 827 1004 1049 1067 1503
Size: 247 580 177 45 18 436
Fragments arranged by size:
580 436 247 177 45 18
SfoI GGC'GCC
Cuts at: 0 1422 1503
Size: 1422 81
SmaI CCC'GGG
Cuts at: 0 1044 1503
Size: 1044 459
SmlI C'TyrA_G
Cuts at: 0 220 415 436 1503
Size: 220 195 21 1067
Fragments arranged by size:
1067 220 195 21
StyI C'CwwG_G
Cuts at: 0 122 1361 1503
Size: 122 1239 142
Fragments arranged by size:
1239 142 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1351 1503
Size: 1351 152
TatI w'GTAC_w
Cuts at: 0 281 629 1503
Size: 281 348 874
Fragments arranged by size:
874 348 281
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 584 616 1503
Size: 584 32 887
Fragments arranged by size:
887 584 32
Tth111I GACn'n_nGTC
Cuts at: 0 784 1503
Size: 784 719
XmaI C'CCGG_G
Cuts at: 0 1042 1503
Size: 1042 461
XmnI GAAnn'nnTTC
Cuts at: 0 706 1503
Size: 706 797
Enzymes that do cut:
AarI AcuI AfeI AlfI AlwNI ApaLI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgI BtsI
CspCI EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI
NgoMIV PfoI PpuMI PsrI PstI PvuII SanDI ScaI
SexAI SfcI SfoI SmaI SmlI StyI TaqII TatI
TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BbsI BciVI BclI BfrBI BglII BmgBI BmtI
BsaI BsaAI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BsrBI BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI
DraI DraIII DrdI EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SapI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XcmI XhoI ZraI