Restriction map for
pFN21AE0956
(Linear) (Six-base) MAPSORT of: KIEE0956.seq Check: 643 from: 1 to: 1458
KIEE0956.orf
With 182 enzymes: SgfI *
January 29, 2014 11:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 551 1458
Size: 551 907
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1342 1458
Size: 1342 116
AflIII A'CryG_T
Cuts at: 0 494 1458
Size: 494 964
AhdI GACnn_n'nnGTC
Cuts at: 0 834 1458
Size: 834 624
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 481 513 1458
Size: 481 32 945
Fragments arranged by size:
945 481 32
ApaI G_GGCC'C
Cuts at: 0 135 1458
Size: 135 1323
AvaI C'yCGr_G
Cuts at: 0 45 128 134 141 1009 1220 1341 1458
Size: 45 83 6 7 868 211 121 117
Fragments arranged by size:
868 211 121 117 83 45 7 6
BanI G'GyrC_C
Cuts at: 0 10 754 1458
Size: 10 744 704
Fragments arranged by size:
744 704 10
BanII G_rGCy'C
Cuts at: 0 52 135 208 1458
Size: 52 83 73 1250
Fragments arranged by size:
1250 83 73 52
BbeI G_GCGC'C
Cuts at: 0 14 1458
Size: 14 1444
BbsI GAAGACnn'nnnn_
Cuts at: 0 405 444 1458
Size: 405 39 1014
Fragments arranged by size:
1014 405 39
BglI GCCn_nnn'nGGC
Cuts at: 0 80 1458
Size: 80 1378
BglII A'GATC_T
Cuts at: 0 779 1458
Size: 779 679
Bme1580I G_kGCm'C
Cuts at: 0 135 1115 1458
Size: 135 980 343
Fragments arranged by size:
980 343 135
BmgBI CAC'GTC
Cuts at: 0 261 450 1038 1458
Size: 261 189 588 420
Fragments arranged by size:
588 420 261 189
BmrI ACTGGGnnnn_n'
Cuts at: 0 529 1239 1458
Size: 529 710 219
Fragments arranged by size:
710 529 219
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1367 1458
Size: 1367 91
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 879 1008 1015 1174 1189 1458
Size: 445 434 129 7 159 15 269
Fragments arranged by size:
445 434 269 159 129 15 7
Bpu10I CC'TnA_GC
Cuts at: 0 1081 1458
Size: 1081 377
BsaAI yAC'GTr
Cuts at: 0 959 1458
Size: 959 499
BsaHI Gr'CG_yC
Cuts at: 0 11 40 1458
Size: 11 29 1418
Fragments arranged by size:
1418 29 11
BsaWI w'CCGG_w
Cuts at: 0 774 1458
Size: 774 684
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 337 367 481 511 877 907 1006 1036
Size: 337 30 114 30 366 30 99 30
Cuts at: 1036 1458
Size: 422
Fragments arranged by size:
422 366 337 114 99 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 502 985 1171 1341 1458
Size: 18 484 483 186 170 117
Fragments arranged by size:
484 483 186 170 117 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 577 940 1060 1114 1168 1458
Size: 349 228 363 120 54 54 290
Fragments arranged by size:
363 349 290 228 120 54 54
BsiEI CG_ry'CG
Cuts at: 0 66 1448 1458
Size: 66 1382 10
Fragments arranged by size:
1382 66 10
BsiHKAI G_wGCw'C
Cuts at: 0 449 1016 1458
Size: 449 567 442
Fragments arranged by size:
567 449 442
Bsp1286I G_dGCh'C
Cuts at: 0 52 135 208 449 1016 1115 1458
Size: 52 83 73 241 567 99 343
Fragments arranged by size:
567 343 241 99 83 73 52
BspEI T'CCGG_A
Cuts at: 0 774 1458
Size: 774 684
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 521 539 551 626 1458
Size: 521 18 12 75 832
Fragments arranged by size:
832 521 75 18 12
BsrBI CCG'CTC
Cuts at: 0 155 735 1454 1458
Size: 155 580 719 4
Fragments arranged by size:
719 580 155 4
BsrDI GCAATG_nn'
Cuts at: 0 707 1458
Size: 707 751
BsrFI r'CCGG_y
Cuts at: 0 79 237 1016 1060 1458
Size: 79 158 779 44 398
Fragments arranged by size:
779 398 158 79 44
BsrGI T'GTAC_A
Cuts at: 0 584 1458
Size: 584 874
BssHII G'CGCG_C
Cuts at: 0 68 70 1458
Size: 68 2 1388
Fragments arranged by size:
1388 68 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 320 555 1458
Size: 320 235 903
Fragments arranged by size:
903 320 235
BstXI CCAn_nnnn'nTGG
Cuts at: 0 972 1458
Size: 972 486
BstYI r'GATC_y
Cuts at: 0 281 428 779 1458
Size: 281 147 351 679
Fragments arranged by size:
679 351 281 147
BtgI C'CryG_G
Cuts at: 0 35 905 1458
Size: 35 870 553
Fragments arranged by size:
870 553 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 161 1458
Size: 161 1297
BtsI GCAGTG_nn'
Cuts at: 0 388 1131 1458
Size: 388 743 327
Fragments arranged by size:
743 388 327
EaeI y'GGCC_r
Cuts at: 0 63 120 221 624 1058 1205 1445 1458
Size: 63 57 101 403 434 147 240 13
Fragments arranged by size:
434 403 240 147 101 63 57 13
EagI C'GGCC_G
Cuts at: 0 63 1445 1458
Size: 63 1382 13
Fragments arranged by size:
1382 63 13
EarI CTCTTCn'nnn_
Cuts at: 0 190 1084 1318 1458
Size: 190 894 234 140
Fragments arranged by size:
894 234 190 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1401 1417 1458
Size: 1401 16 41
Fragments arranged by size:
1401 41 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 879 1008 1015 1174 1189 1342 1458
Size: 445 434 129 7 159 15 153 116
Fragments arranged by size:
445 434 159 153 129 116 15 7
EcoO109I rG'GnC_Cy
Cuts at: 0 528 878 1178 1335 1458
Size: 528 350 300 157 123
Fragments arranged by size:
528 350 300 157 123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 324 1458
Size: 324 1134
HaeII r_GCGC'y
Cuts at: 0 14 35 1057 1203 1458
Size: 14 21 1022 146 255
Fragments arranged by size:
1022 255 146 21 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 139 171 820 852 877 909
Size: 79 32 28 32 649 32 25 32
Cuts at: 909 1458
Size: 549
Fragments arranged by size:
649 549 79 32 32 32 32 28
25
HincII GTy'rAC
Cuts at: 0 798 1458
Size: 798 660
KasI G'GCGC_C
Cuts at: 0 10 1458
Size: 10 1448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 1458
Size: 110 1348
MscI TGG'CCA
Cuts at: 0 122 1207 1458
Size: 122 1085 251
Fragments arranged by size:
1085 251 122
MslI CAynn'nnrTG
Cuts at: 0 499 505 644 1458
Size: 499 6 139 814
Fragments arranged by size:
814 499 139 6
MspA1I CmG'CkG
Cuts at: 0 37 115 924 1458
Size: 37 78 809 534
Fragments arranged by size:
809 534 78 37
NaeI GCC'GGC
Cuts at: 0 81 1062 1458
Size: 81 981 396
Fragments arranged by size:
981 396 81
NarI GG'CG_CC
Cuts at: 0 11 1458
Size: 11 1447
NgoMIV G'CCGG_C
Cuts at: 0 79 1060 1458
Size: 79 981 398
Fragments arranged by size:
981 398 79
NspI r_CATG'y
Cuts at: 0 498 688 1458
Size: 498 190 770
Fragments arranged by size:
770 498 190
PciI A'CATG_T
Cuts at: 0 494 1458
Size: 494 964
PfoI T'CCnGG_A
Cuts at: 0 416 479 668 1031 1166 1458
Size: 416 63 189 363 135 292
Fragments arranged by size:
416 363 292 189 135 63
PmlI CAC'GTG
Cuts at: 0 959 1458
Size: 959 499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 481 513 1458
Size: 481 32 945
Fragments arranged by size:
945 481 32
PpuMI rG'GwC_Cy
Cuts at: 0 528 1335 1458
Size: 528 807 123
Fragments arranged by size:
807 528 123
PspOMI G'GGCC_C
Cuts at: 0 131 1458
Size: 131 1327
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 454 486 1458
Size: 454 32 972
Fragments arranged by size:
972 454 32
PstI C_TGCA'G
Cuts at: 0 437 548 1458
Size: 437 111 910
Fragments arranged by size:
910 437 111
PvuII CAG'CTG
Cuts at: 0 924 1458
Size: 924 534
SacII CC_GC'GG
Cuts at: 0 38 1458
Size: 38 1420
SbfI CC_TGCA'GG
Cuts at: 0 437 1458
Size: 437 1021
SfcI C'TryA_G
Cuts at: 0 433 544 1458
Size: 433 111 914
Fragments arranged by size:
914 433 111
SfoI GGC'GCC
Cuts at: 0 12 1458
Size: 12 1446
SmaI CCC'GGG
Cuts at: 0 47 130 136 1222 1458
Size: 47 83 6 1086 236
Fragments arranged by size:
1086 236 83 47 6
StyI C'CwwG_G
Cuts at: 0 965 1458
Size: 965 493
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 712 1458
Size: 712 746
TatI w'GTAC_w
Cuts at: 0 365 584 1458
Size: 365 219 874
Fragments arranged by size:
874 365 219
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1006 1038 1458
Size: 1006 32 420
Fragments arranged by size:
1006 420 32
Tth111I GACn'n_nGTC
Cuts at: 0 400 1458
Size: 400 1058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 765 903 972 1149 1458
Size: 765 138 69 177 309
Fragments arranged by size:
765 309 177 138 69
XmaI C'CCGG_G
Cuts at: 0 45 128 134 1220 1458
Size: 45 83 6 1086 238
Fragments arranged by size:
1086 238 83 45 6
Enzymes that do cut:
AarI AcuI AflIII AhdI AloI ApaI AvaI BanI
BanII BbeI BbsI BglI BglII Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstXI BstYI BtgI BtgZI
BtsI EaeI EagI EarI EciI Eco57MI EcoO109I FalI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PmlI PpiI
PpuMI PspOMI PsrI PstI PvuII SacII SbfI SfcI
SfoI SmaI StyI TaqII TatI TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BlpI
BmtI BpuEI BsaI BsaBI BseYI BsiWI BsmI BsmBI
BspHI BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmeI PshAI PsiI PspXI PvuI RsrII SacI SalI
SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI
XhoI XmnI ZraI