Restriction map for
pFN21AE0949
(Linear) (Six-base) MAPSORT of: KIEE0949.seq Check: 9211 from: 1 to: 1293
KIEE0949.orf
With 182 enzymes: SgfI *
July 3, 2012 16:41 ..
AccI GT'mk_AC
Cuts at: 0 626 1293
Size: 626 667
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1063 1293
Size: 559 504 230
Fragments arranged by size:
559 504 230
AflII C'TTAA_G
Cuts at: 0 69 1293
Size: 69 1224
AleI CACnn'nnGTG
Cuts at: 0 581 1293
Size: 581 712
AlwNI CAG_nnn'CTG
Cuts at: 0 861 1293
Size: 861 432
AvaI C'yCGr_G
Cuts at: 0 736 1293
Size: 736 557
BanII G_rGCy'C
Cuts at: 0 139 940 1293
Size: 139 801 353
Fragments arranged by size:
801 353 139
BbvCI CC'TCA_GC
Cuts at: 0 652 920 1293
Size: 652 268 373
Fragments arranged by size:
652 373 268
BciVI GTATCCnnnnn_n'
Cuts at: 0 894 1293
Size: 894 399
BglI GCCn_nnn'nGGC
Cuts at: 0 1162 1293
Size: 1162 131
BglII A'GATC_T
Cuts at: 0 468 1293
Size: 468 825
BlpI GC'TnA_GC
Cuts at: 0 730 1293
Size: 730 563
Bme1580I G_kGCm'C
Cuts at: 0 890 1293
Size: 890 403
BmgBI CAC'GTC
Cuts at: 0 1153 1293
Size: 1153 140
BmrI ACTGGGnnnn_n'
Cuts at: 0 1097 1293
Size: 1097 196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 742 1291 1293
Size: 730 12 549 2
Fragments arranged by size:
730 549 12 2
Bpu10I CC'TnA_GC
Cuts at: 0 652 920 1293
Size: 652 268 373
Fragments arranged by size:
652 373 268
BsaI GGTCTCn'nnnn_
Cuts at: 0 48 1293
Size: 48 1245
BsaAI yAC'GTr
Cuts at: 0 288 1293
Size: 288 1005
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 272 302 1003 1033 1293
Size: 272 30 701 30 260
Fragments arranged by size:
701 272 260 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 937 1293
Size: 937 356
BsiHKAI G_wGCw'C
Cuts at: 0 139 1293
Size: 139 1154
Bsp1286I G_dGCh'C
Cuts at: 0 139 890 940 1293
Size: 139 751 50 353
Fragments arranged by size:
751 353 139 50
BspHI T'CATG_A
Cuts at: 0 173 1293
Size: 173 1120
BsrGI T'GTAC_A
Cuts at: 0 478 573 1293
Size: 478 95 720
Fragments arranged by size:
720 478 95
BstAPI GCAn_nnn'nTGC
Cuts at: 0 534 1293
Size: 534 759
BstXI CCAn_nnnn'nTGG
Cuts at: 0 330 1293
Size: 330 963
BstYI r'GATC_y
Cuts at: 0 468 1293
Size: 468 825
BtgI C'CryG_G
Cuts at: 0 666 724 1293
Size: 666 58 569
Fragments arranged by size:
666 569 58
BtsI GCAGTG_nn'
Cuts at: 0 1078 1293
Size: 1078 215
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 393 428 1293
Size: 393 35 865
Fragments arranged by size:
865 393 35
DraIII CAC_nnn'GTG
Cuts at: 0 1065 1293
Size: 1065 228
EaeI y'GGCC_r
Cuts at: 0 815 822 953 1293
Size: 815 7 131 340
Fragments arranged by size:
815 340 131 7
EarI CTCTTCn'nnn_
Cuts at: 0 376 1259 1293
Size: 376 883 34
Fragments arranged by size:
883 376 34
EcoICRI GAG'CTC
Cuts at: 0 137 1293
Size: 137 1156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 730 742 1063 1291 1293
Size: 559 171 12 321 228 2
Fragments arranged by size:
559 321 228 171 12 2
EcoO109I rG'GnC_Cy
Cuts at: 0 240 543 741 1293
Size: 240 303 198 552
Fragments arranged by size:
552 303 240 198
FspI TGC'GCA
Cuts at: 0 643 1293
Size: 643 650
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 121 153 240 272 760 792 1003 1035
Size: 121 32 87 32 488 32 211 32
Cuts at: 1035 1293
Size: 258
Fragments arranged by size:
488 258 211 121 87 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 314 1293
Size: 314 979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 1028 1293
Size: 878 150 265
Fragments arranged by size:
878 265 150
MscI TGG'CCA
Cuts at: 0 817 955 1293
Size: 817 138 338
Fragments arranged by size:
817 338 138
MslI CAynn'nnrTG
Cuts at: 0 581 856 1293
Size: 581 275 437
Fragments arranged by size:
581 437 275
MspA1I CmG'CkG
Cuts at: 0 675 919 1293
Size: 675 244 374
Fragments arranged by size:
675 374 244
NcoI C'CATG_G
Cuts at: 0 666 1293
Size: 666 627
NspI r_CATG'y
Cuts at: 0 361 1293
Size: 361 932
PflMI CCAn_nnn'nTGG
Cuts at: 0 564 1065 1293
Size: 564 501 228
Fragments arranged by size:
564 501 228
PpuMI rG'GwC_Cy
Cuts at: 0 240 741 1293
Size: 240 501 552
Fragments arranged by size:
552 501 240
PstI C_TGCA'G
Cuts at: 0 530 1293
Size: 530 763
SacI G_AGCT'C
Cuts at: 0 139 1293
Size: 139 1154
SapI GCTCTTCn'nnn_
Cuts at: 0 1259 1293
Size: 1259 34
SbfI CC_TGCA'GG
Cuts at: 0 530 1293
Size: 530 763
SfcI C'TryA_G
Cuts at: 0 526 786 1293
Size: 526 260 507
Fragments arranged by size:
526 507 260
SmlI C'TyrA_G
Cuts at: 0 69 1293
Size: 69 1224
StyI C'CwwG_G
Cuts at: 0 666 1293
Size: 666 627
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 868 1293
Size: 868 425
TatI w'GTAC_w
Cuts at: 0 478 573 704 1293
Size: 478 95 131 589
Fragments arranged by size:
589 478 131 95
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 272 304 1293
Size: 272 32 989
Fragments arranged by size:
989 272 32
XmnI GAAnn'nnTTC
Cuts at: 0 1178 1293
Size: 1178 115
Enzymes that do cut:
AccI AcuI AflII AleI AlwNI AvaI BanII BbvCI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BsaI BsaAI BsaXI BseRI BsiHKAI Bsp1286I BspHI
BsrGI BstAPI BstXI BstYI BtgI BtsI CspCI DraIII
EaeI EarI EcoICRI Eco57MI EcoO109I FspI Hin4I HindIII
MmeI MscI MslI MspA1I NcoI NspI PflMI PpuMI
PstI SacI SapI SbfI SfcI SmlI StyI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BclI
BfrBI BmtI BplI BpuEI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI DraI DrdI EagI EciI EcoNI EcoRI
EcoRV FalI FseI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI