Restriction map for
pFN21AE0933
(Linear) (Six-base) MAPSORT of: KIEE0933.seq Check: 7774 from: 1 to: 1245
KIEE0933.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AccI GT'mk_AC
Cuts at: 0 686 852 1245
Size: 686 166 393
Fragments arranged by size:
686 393 166
AleI CACnn'nnGTG
Cuts at: 0 170 647 1245
Size: 170 477 598
Fragments arranged by size:
598 477 170
AlwNI CAG_nnn'CTG
Cuts at: 0 60 1245
Size: 60 1185
ApaLI G'TGCA_C
Cuts at: 0 368 1245
Size: 368 877
ApoI r'AATT_y
Cuts at: 0 71 1245
Size: 71 1174
AseI AT'TA_AT
Cuts at: 0 206 1245
Size: 206 1039
BanI G'GyrC_C
Cuts at: 0 670 1245
Size: 670 575
BbsI GAAGACnn'nnnn_
Cuts at: 0 319 890 1245
Size: 319 571 355
Fragments arranged by size:
571 355 319
BciVI GTATCCnnnnn_n'
Cuts at: 0 30 1245
Size: 30 1215
BclI T'GATC_A
Cuts at: 0 162 903 1245
Size: 162 741 342
Fragments arranged by size:
741 342 162
Bme1580I G_kGCm'C
Cuts at: 0 372 1245
Size: 372 873
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1039 1245
Size: 1039 206
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 1114 1245
Size: 671 443 131
Fragments arranged by size:
671 443 131
BsiHKAI G_wGCw'C
Cuts at: 0 372 1044 1245
Size: 372 672 201
Fragments arranged by size:
672 372 201
Bsp1286I G_dGCh'C
Cuts at: 0 372 1044 1245
Size: 372 672 201
Fragments arranged by size:
672 372 201
BsrBI CCG'CTC
Cuts at: 0 18 1245
Size: 18 1227
BsrGI T'GTAC_A
Cuts at: 0 988 1245
Size: 988 257
BstBI TT'CG_AA
Cuts at: 0 26 75 1245
Size: 26 49 1170
Fragments arranged by size:
1170 49 26
BstEII G'GTnAC_C
Cuts at: 0 979 1245
Size: 979 266
BstZ17I GTA'TAC
Cuts at: 0 853 1245
Size: 853 392
BtgI C'CryG_G
Cuts at: 0 624 1245
Size: 624 621
DraI TTT'AAA
Cuts at: 0 408 591 1245
Size: 408 183 654
Fragments arranged by size:
654 408 183
EaeI y'GGCC_r
Cuts at: 0 107 379 538 1245
Size: 107 272 159 707
Fragments arranged by size:
707 272 159 107
EarI CTCTTCn'nnn_
Cuts at: 0 23 224 1245
Size: 23 201 1021
Fragments arranged by size:
1021 201 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 903 1245
Size: 903 342
HaeII r_GCGC'y
Cuts at: 0 666 1205 1245
Size: 666 539 40
Fragments arranged by size:
666 539 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 436 468 771 803 1245
Size: 340 32 64 32 303 32 442
Fragments arranged by size:
442 340 303 64 32 32 32
HincII GTy'rAC
Cuts at: 0 222 1245
Size: 222 1023
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 1245
Size: 14 1231
MscI TGG'CCA
Cuts at: 0 109 381 540 1245
Size: 109 272 159 705
Fragments arranged by size:
705 272 159 109
MslI CAynn'nnrTG
Cuts at: 0 170 647 1171 1245
Size: 170 477 524 74
Fragments arranged by size:
524 477 170 74
MspA1I CmG'CkG
Cuts at: 0 60 1245
Size: 60 1185
NcoI C'CATG_G
Cuts at: 0 624 1245
Size: 624 621
NdeI CA'TA_TG
Cuts at: 0 159 1245
Size: 159 1086
NspI r_CATG'y
Cuts at: 0 281 1245
Size: 281 964
PflMI CCAn_nnn'nTGG
Cuts at: 0 547 1245
Size: 547 698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 501 533 1245
Size: 7 32 462 32 712
Fragments arranged by size:
712 462 32 32 7
PshAI GACnn'nnGTC
Cuts at: 0 692 1245
Size: 692 553
ScaI AGT'ACT
Cuts at: 0 376 1245
Size: 376 869
SfcI C'TryA_G
Cuts at: 0 645 1245
Size: 645 600
StyI C'CwwG_G
Cuts at: 0 624 1245
Size: 624 621
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 998 1245
Size: 998 247
TatI w'GTAC_w
Cuts at: 0 62 374 465 988 1245
Size: 62 312 91 523 257
Fragments arranged by size:
523 312 257 91 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 410 442 1245
Size: 410 32 803
Fragments arranged by size:
803 410 32
XbaI T'CTAG_A
Cuts at: 0 957 1245
Size: 957 288
Enzymes that do cut:
AccI AleI AlwNI ApaLI ApoI AseI BanI BbsI
BciVI BclI Bme1580I BseRI BsgI BsiHKAI Bsp1286I BsrBI
BsrGI BstBI BstEII BstZ17I BtgI DraI EaeI EarI
FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I
NcoI NdeI NspI PflMI PpiI PshAI ScaI SfcI
StyI TaqII TatI TstI XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI AscI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbvCI BcgI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpuMI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XcmI XhoI XmaI XmnI ZraI