(Linear) (Six-base) MAPSORT of: KIEE0905.seq  Check: 9212  from: 1  to: 531



KIEE0905.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:33  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     483     531 
   Size:        483      48
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     110     142     531 
   Size:        110      32     389

  Fragments arranged by size: 

                 389     110      32
 
AvaI C'yCGr_G

Cuts at:      0     388     531 
   Size:        388     143
 
BanI G'GyrC_C

Cuts at:      0      57     531 
   Size:         57     474
 
BanII G_rGCy'C

Cuts at:      0     515     531 
   Size:        515      16
 
BlpI GC'TnA_GC

Cuts at:      0      46     531 
   Size:         46     485
 
Bme1580I G_kGCm'C

Cuts at:      0      62     531 
   Size:         62     469
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     467     531 
   Size:        467      64
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     470     529     531 
   Size:        470      59       2

  Fragments arranged by size: 

                 470      59       2
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     531 
   Size:        340     191
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      91     531 
   Size:         91     440
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     110     140     531 
   Size:        110      30     391

  Fragments arranged by size: 

                 391     110      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     531 
   Size:        118     413
 
BsiHKAI G_wGCw'C

Cuts at:      0     515     531 
   Size:        515      16
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     328     531 
   Size:        328     203
 
Bsp1286I G_dGCh'C

Cuts at:      0      62     515     531 
   Size:         62     453      16

  Fragments arranged by size: 

                 453      62      16
 
BsrBI CCG'CTC

Cuts at:      0     354     531 
   Size:        354     177
 
BtsI GCAGTG_nn'

Cuts at:      0      28     531 
   Size:         28     503
 
EcoICRI GAG'CTC

Cuts at:      0     513     531 
   Size:        513      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     470     529     531 
   Size:        470      59       2

  Fragments arranged by size: 

                 470      59       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     313     531 
   Size:        313     218
 
HaeII r_GCGC'y

Cuts at:      0      75     531 
   Size:         75     456
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     469     501     531 
   Size:        469      32      30

  Fragments arranged by size: 

                 469      32      30
 
MspA1I CmG'CkG

Cuts at:      0       6     208     531 
   Size:          6     202     323

  Fragments arranged by size: 

                 323     202       6
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     110     142     531 
   Size:        110      32     389

  Fragments arranged by size: 

                 389     110      32
 
PvuII CAG'CTG

Cuts at:      0     208     531 
   Size:        208     323
 
SacI G_AGCT'C

Cuts at:      0     515     531 
   Size:        515      16
 
SmaI CCC'GGG

Cuts at:      0     390     531 
   Size:        390     141
 
SmlI C'TyrA_G

Cuts at:      0     355     531 
   Size:        355     176
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     504     531 
   Size:        504      27
 
XmaI C'CCGG_G

Cuts at:      0     388     531 
   Size:        388     143
 

 Enzymes that do cut:

     AhdI     AloI     AvaI     BanI    BanII     BlpI Bme1580I     BmrI
     BpmI    BpuEI     BsaI    BsaXI    BseRI  BsiHKAI    BsmBI Bsp1286I
    BsrBI     BtsI  EcoICRI  Eco57MI    EcoNI    HaeII    Hin4I   MspA1I
     PpiI    PvuII     SacI     SmaI     SmlI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI
     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmnI     ZraI